LOCUS 40924_2154 6465 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector mTol2-B-P-GFF-CR3, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6465) AUTHORS Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, Chien CB, Dorsky R. TITLE Large Scale Zebrafish Gal4 Enhancer Trap Screen for Neural Specific Expression Patterns JOURNAL Unpublished REFERENCE 2 (bases 1 to 6465) AUTHORS Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, Chien CB, Dorsky R. TITLE Direct Submission JOURNAL Submitted (19-MAR-2013) Neurobiology and Anatomy, University of Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA REFERENCE 3 (bases 1 to 6465) TITLE Direct Submission REFERENCE 4 (bases 1 to 6465) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (19-MAR-2013) Neurobiology and Anatomy, University of Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..6465 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 181..636 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 714..818 /label=AmpR promoter CDS 819..1676 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1850..2438 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2726..2747 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2762..2792 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2800..2816 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2824..2840 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2858..2876 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" protein_bind 3144..3164 /label=attB4 /note="core recombination site for the Gateway(R) BP reaction" protein_bind 3248..3272 /label=attB1 /note="recombination site for the Gateway(R) BP reaction" CDS 3281..3721 /codon_start=1 /label=GAL4 DNA binding domain /note="DNA binding domain of the GAL4 transcriptional activator" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" protein_bind complement(3822..3846) /label=attB2 /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4045..4065) /label=attB3 /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(4073..4089) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" polyA_signal complement(4105..4239) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 4429..4563 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(4604..5314) /codon_start=1 /label=TagRFP /note="monomeric derivative of red fluorescent protein from Entacmaea quadricolor (Merzlyak et al., 2007)" /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV ARYCDLPSKLGHKLN" promoter complement(5315..6213) /label=cmlc2 /note="Cardiac myosin light chain 2 promoter" promoter complement(join(6464..6465,1..17)) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac 661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa 721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac 2881 tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgagcaga ggtgtaaaaa 2941 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta 3001 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa 3061 attgtacttg agtattaagg aagtagggat aacagggtaa tctcgagcag gaaacagcta 3121 tgaccatgat tacgccaagc tatcaacttt gtatagaaaa gttgggccgg cccttcggcg 3181 cgccactcta gagggtatat aatggatctc gcgtctcagc ctcactttga gctcctccac 3241 accgcgccca agtttgtaca aaaaagcagg ctcggccacc atgaagctac tgtcttctat 3301 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 3361 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 3421 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 3481 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 3541 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 3601 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 3661 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 3721 gccggaattc ccggccgacg ccctggacga cttcgacctg gacatgctgc ctgctgatgc 3781 tctcgatgat ttcgatctcg atatgctccc gggtaactaa gacccagctt tcttgtacaa 3841 agtgggggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat 3901 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 3961 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca 4021 gggggaggtg tgggaggttt tttccaactt tattatacat agttgataat tcactggccg 4081 tcgttttacg gtaccatcga tgatgatcca gacatgataa gatacattga tgagtttgga 4141 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt 4201 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat 4261 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac 4321 aaatgtggta tggctgatta tgatcctcta gatcagatct gcgaattaaa aaacctccca 4381 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt 4441 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 4501 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 4561 atctacgtaa tacgactcac tatagttcta gaggcgcgcc tcaattaagt ttgtgcccca 4621 gtttgctagg gaggtcgcag tatctggcca cagccacctc gtgctgctcg acgtaggtct 4681 ctttgtcggc ctccttgatt ctttccagtc tgtggtccac atagtagacg ccgggcatct 4741 tgaggttctt agcgggtttc ttggatctgt atgtggtctt gaagttgcag atcaggtggc 4801 ccccgcccac gagcttcagg gccatgtcgc ttctgccttc caggccgccg tcagcggggt 4861 acagcatctc ggtgttggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg 4921 atgggaagtt cacccctctg atcttgacgt tgtagatgag gcagccgtcc tggaggctgg 4981 tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt ggtgactctc tcccatgtga 5041 agccctcagg gaaggactgc ttaaagaagt cggggatgcc ctgggtgtgg ttgatgaagg 5101 ttctgctgcc gtacatgaag ctggtagcca ggatgtcgaa ggcgaagggg agagggccgc 5161 cctcgaccac cttgattctc atggtctggg tgccctcgta gggcttgcct tcgccctcgg 5221 atgtgcactt gaagtggtgg ttgttcacgg tgccctccat gtacagcttc atgtgcatgt 5281 tctccttaat cagctcttcg cccttagaca ccatggtcac tgtctgcttt gctgttggtc 5341 tgggctcctg ggtcactggc ttactaatgg agtctttatg tatgaggact cttatcaatt 5401 gttcttctat aaaggtctgc agtgtttctg ttcgtcccct acatggacac ccagagcctc 5461 ctaaatacag gagccctgat aactgcacaa gtgctcagat tccagcaggg tggaaaatga 5521 gataaagtgt gcagatgggg agggggacgt gaatgagaga tttgagggat gaaaaggatg 5581 gatgaacgca ttgaaaatag cccctttcac aaaataatac cagtaaattg tcatataatt 5641 aatgaccaga cctttacctg taaatgatgt gatgttcata tacagaagaa tgccaacaat 5701 ttacagatga ttttacaaat tcttaatttt ccttttctaa atggatttgt tcacacatta 5761 tctcttcaaa gcaattgaat tttctggaaa agattggctg tgtgtgtaaa aggtgctact 5821 gaggaattta atgtgacatg gaatgaagca aacagcagcc taagacagga tggcaggaaa 5881 aatgtcacat gtttaaaata agagtgaaac caacgacctg gacttataga gactgtgtgt 5941 ccttaggcaa agctcttgta caaacagtgc ttgtgactgt gaaattcggg gtttgcctgg 6001 attgtgttaa atgtgtgctt ggatgtatca taaaaaagaa tcggttctct gtatttgaca 6061 ttttctgtaa tttatctaaa gttacaactg gctatgcctg atttttatca ctattgaatc 6121 aatcatttta aagaatgtta agtctgctga tttggagagc tcatttacat ttattttgaa 6181 tgtctcttat ttaacacaac tgatttaagc tttagatcta ttaccctgtt atccctaggg 6241 aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa 6301 ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat 6361 ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca 6421 agtaaaatta ctcaagtact ttacacctct gggcccaatt cgccc //