LOCUS       Exported                3464 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector mKate_integration, complete sequence.
ACCESSION   KU904417
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Yu J, Pertusi D, Adeniran A, Tyo KEJ.
  TITLE     CellSort: A support vector machine algorithm for optimizing and 
            simulating FACS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Tyo KEJ., Adeniran A, Stainbrook SC, Bostick JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2016) Chemical and Biological Engineering, 
            Northwestern University, 2145 Sheridan Rd, Evanston, IL 60208, USA
REFERENCE   3  (bases 1 to 3464)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Cloning vector mKate_integration"
                     /mol_type="other DNA"
                     /label=derived from Saccharomyces cerevisiae sequence
                     /note="derived from Saccharomyces cerevisiae sequence"
                     /db_xref="taxon:1814216"
     gene            46..822
                     /gene="TRP1"
                     /label=TRP1
     regulatory      46..147
                     /regulatory_class="promoter"
                     /gene="TRP1"
     promoter        46..147
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             148..822
                     /codon_start=1
                     /gene="TRP1"
                     /product="TRP1"
                     /label=TRP1
                     /protein_id="AMY56670.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      661..1498
                     /note="Saccharomyces cerevisiae autonomously replicating 
                     sequence ARS1/ARS416"
     regulatory      1499..1686
                     /regulatory_class="terminator"
                     /gene="ADH1"
     gene            1499..1686
                     /gene="ADH1"
                     /label=ADH1
     terminator      1499..1686
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     regulatory      1819..2454
                     /regulatory_class="promoter"
                     /gene="TDH3"
     gene            1819..2454
                     /gene="TDH3"
                     /label=TDH3
     CDS             2467..3177
                     /codon_start=1
                     /product="TagRFP"
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /protein_id="AMY56669.1"
                     /translation="MSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     regulatory      3198..3385
                     /regulatory_class="terminator"
                     /gene="ADH1"
     gene            3198..3385
                     /gene="ADH1"
                     /label=ADH1
     terminator      3198..3385
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
ORIGIN
        1 tacgtacaat cttgatccgg agcttttctt tttttgccga ttaagaattc ggtcgaaaaa
       61 agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg
      121 attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt
      181 ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc
      241 gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt
      301 attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa
      361 tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat
      421 tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc
      481 ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt
      541 gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca
      601 ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa
      661 agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga
      721 ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac
      781 tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat
      841 ttatttaagt attgtttgtg cacttgcctg caggcctttt gaaaagcaag cataaaagat
      901 ctaaacataa aatctgtaaa ataacaagat gtaaagataa tgctaaatca tttggctttt
      961 tgattgattg tacaggaaaa tatacatcgc agggggttga cttttaccat ttcaccgcaa
     1021 tggaatcaaa cttgttgaag agaatgttca caggcgcata cgctacaatg acccgattct
     1081 tgctagcctt ttctcggtct tgcaaacaac cgccggcagc ttagtatata aatacacatg
     1141 tacatacctc tctccgtatc ctcgtaatca ttttcttgta tttatcgtct tttcgctgta
     1201 aaaactttat cacacttatc tcaaatacac ttattaaccg cttttactat tatcttctac
     1261 gctgacagta atatcaaaca gtgacacata ttaaacacag tggtttcttt gcataaacac
     1321 catcagcctc aagtcgtcaa gtaaagattt cgtgttcatg cagatagata acaatctata
     1381 tgttgataat tagcgttgcc tcatcaatgc gagatccgtt taaccggacc ctagtgcact
     1441 taccccacgt tcggtccact gtgtgccgaa catgctcctt cactatttta acatgtgggc
     1501 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
     1561 caaattttaa agtgactctt aggttttaaa acgaaaattc ttgttcttga gtaactcttt
     1621 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc
     1681 taccggcatg ccgagcaaat gcctgcaaat cgctccccat ttcacccaat tgtagatatg
     1741 ctaactccag caatgagttg atgaatctcg gtgtgtattt tatgtcctca gaggacaaca
     1801 cctgttgtaa tcgagctcag tttatcatta tcaatactcg ccatttcaaa gaatacgtaa
     1861 ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc ttttaattct
     1921 gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat aacatcgtag
     1981 gtgtctgggt gaacagttta ttcctggcat ccactaaata taatggagcc cgctttttaa
     2041 gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat
     2101 cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa acaggcaaaa
     2161 aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg acacaaggca
     2221 attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc
     2281 tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctact
     2341 tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta
     2401 aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac accaggatcc
     2461 aacaaaatgt ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg
     2521 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
     2581 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     2641 gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc
     2701 atccccgact tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca
     2761 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     2821 atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag
     2881 aaaacactcg gctgggaggc ctccaccgag atgctgtacc ccgctgacgg cggcctggaa
     2941 ggcagaagcg acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag
     3001 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg
     3061 gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     3121 gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact taattgaggc
     3181 gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
     3241 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
     3301 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg
     3361 ctcttattga ccacacctct accggcagat ccgctaggga taacagggta atataattct
     3421 tgaaagaatg aaatcgccat gccaagccat cacacggtct ttta
//
