LOCUS       62056_1575        7516 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3107..3814
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    4304..4425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4432..4887)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4914..5018
                     /label=AmpR promoter
     promoter        5020..5377
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5412..6203
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6438..6485
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6814..7402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catgtggcta ggctggtcaa gctggtttta gctggtcatc tcccagactg taattaaaaa
      421 agtaatttaa atataacttt atgatgcaaa aataattatt tacttttaca ataaacattt
      481 gattaattta gcatataaat acagttaaaa tcagaattat taaaccccat ttaatttttt
      541 ttcgttattt atttttccca tatgatgttt aacagagcaa ggaaattttc acattatatc
      601 tgataatatt ttttcttctg aagaaagtct tttttttaat ttcagctaga ataaaagcag
      661 tttctatttt ttttaaacta tttagctcaa aattattagc ttatttaggc tacatcttta
      721 gtctacagaa caaaccattg ttatacaata acttgcctta ttagccaata ctaccatatt
      781 ctgcctagtt accctaatta acctagtgaa gcctttaaac atcactttaa actgtataaa
      841 actgtcttgg aaaatatcta gtcaaataca attttctgtc atcatggcaa atataaaata
      901 aagcagttat tagagatgcg ttatcaaaat atgtttaaaa atgtgttgaa aaaatactct
      961 ctctgttaaa cagaaattgg gggaacaata aacaaggggg gctaataatt caggagggct
     1021 attaattctg acttcattat atatatatat tagtacaccc aaattaatgt ttgacaggaa
     1081 aaatcaagag aaacataaat catatagcat ttaattgaaa tgtcgttgtg tgtaatttta
     1141 ttgttgttga aatagctcgt attttctttc tattcctaaa gatggtgtgt gaaccaacac
     1201 ttattttaat ggatatatat gttaaataaa cacccgtttt gtttaaatgc accaaaattt
     1261 accttttgtt atgctaattt tctaagggcg tgaagagaca gagacctatg ctaatttata
     1321 aaaaaatatc attacatgca atttatttta actgacttca cgtatacatg gacattactt
     1381 ccactatcat aaaaaaagga aaaaaagaaa aagaaagaaa gaaatgacaa acctcaagtt
     1441 ccctgcaaca aggttaaatg aaatttagaa tcttcaagta actttcggca acagttaaaa
     1501 tggcagatta atgatgaaac cattcgactt ctgaagcgca tctaaagatc tcgcacacac
     1561 tacggaaaat gtaaattaag atgaaccgat gagatatgaa tggggcattt ttaggttatg
     1621 cttgttaaat attcatgagc tgaaacttta tcttgagatg gatagggaca tgactttatc
     1681 ataaaaggtt tcatacgcta gattagtgaa tatcatgaca cagttggcac aacaagactg
     1741 ttcagaattt tgccaagact taaaaaaaaa ggtacgcgct gttaatctgc gtaatgaatg
     1801 agaaaatgcc agagaggtaa tggatgcccg ccacatagca gatgaataat tgagcgtctg
     1861 ggggaacagg agaggtggat gggatcggat ggtgcatctg tgtcactgca ctgcctggct
     1921 tctctttcta cagccaccat cacggtcacc atggaggtat aacaggtgtt tacttccaac
     1981 taccagaaac atgtttgcaa tgtcatttac caaaccactt tggtggggtg ttcatttaaa
     2041 gttttttttt tatatgacat gctgtttaaa gcttatattt tatatttcat tcgattgact
     2101 tgcatcagcc aaaggctaag aacttgacaa atatatttga tcgatctata ttaaattaat
     2161 ataaaagcat aattgtgttc atttttaatg ccctatagct gtgaccaggg caggtgagag
     2221 cagctctgtc acattcaaca gccaatgaag attaggggtc agccaaggtg aaagttcgtg
     2281 gagtagactg tgagcttcaa ctttcattgc aagttcaaat ggatttaaat acaaatacaa
     2341 atcaaaatgt ggtagcctgc tatatatttg tgttatttta ataatgaaca caatattcta
     2401 cacatgatag tttatttaca taaaaacaaa gaattgtcat aaaaaaaaca tatatagtat
     2461 agccatgctt gtgcattaaa gtggacatgt ataattctgg agatacataa gggtattttt
     2521 aattgttata agcagcctac tgtaaataat tattgtactg taattcataa agtaaaaccg
     2581 aaaaattatt tacagaatta cacttctgtc tacagtttac catccgattt cctattttgg
     2641 catttgaaaa caaagacatt taataaattt tgcattgcta ttatcagatt aaaaactgat
     2701 ctgcatttta atatgttaaa ataaaaatat aaattattac attttttaat taattaatta
     2761 atatcaattt atttatttaa tcaacgtcaa taaaatcatt agttttatta ttatgctaaa
     2821 tatgtcaata tttattttta tttttttaaa tcacttcgtt gaaatactgg gttaaataac
     2881 ctacgaaatc ccacgcgtga gtgtcagacc agtttgccac ttgtatcccg agtagatgga
     2941 ggcgggaggg cagtgtgagt ttggggtggg gggagaaccc gtgaggcgct gccaatggaa
     3001 actgaacaca cacgaggagc gtcgagccag agcgccgcgt ttcctccatg tcaggtccac
     3061 tcacctgcaa ggtgcgcaac gaaggctagc gctaccggtc gccaccatgg tgagcaaggg
     3121 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     3181 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3241 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3301 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3361 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3421 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3481 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     3541 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3601 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     3661 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     3721 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3781 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatcca cggcaagcac
     3841 ggaaaacttt aaaaatgtta aagaaaaata tcagcaaatg cgaggtgatg ctttaaaaac
     3901 agaaatcctg gctgatttca aggataaact ggctgaagct accgatgaac agagccttaa
     3961 gcaaattgtt gctgaattga aaagtaaaga tgaatataga attttggcta aggggcaagg
     4021 gttaacaacc cagcttttag ggttaaagac aagttcagtg tcttcatttg agaaaatggt
     4081 tgaggaaaca agagaaagta ttaagtctca agaaagacaa accattaaga taaaataaga
     4141 attcccgggt cgactcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
     4201 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4261 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4321 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4381 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     4441 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     4501 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     4561 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     4621 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     4681 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     4741 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     4801 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     4861 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4981 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     5041 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5101 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5161 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5221 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5281 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5341 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     5401 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5461 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5521 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5581 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5641 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5701 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5761 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5821 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5881 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     5941 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     6001 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6061 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6121 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6181 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     6241 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6301 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6361 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     6421 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     6481 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6541 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     6601 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     6661 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6721 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6781 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6841 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6901 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6961 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7021 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7081 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7141 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7201 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7261 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7321 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7381 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7441 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7501 tattaccgcc atgcat
//
