LOCUS       V014538                 6005 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014538
VERSION     V014538
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             750..776
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             777..788
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1173..1880
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     intron          1960..2532
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2536..2658
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2729..2784
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     promoter        complement(3139..3157)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3164..3180)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3322..3777
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3803..3907
                     /label="AmpR promoter"
     misc_feature    4092..4471
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      4940..5528
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5816..5837
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5852..5882
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5890..5906
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5914..5930
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5951..5969
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tctagagtcg acctgcagcc caagcttggg ctgaacatcg agcgccaggg ctccgtaaag
       61 ctactagagc acaggcggtg ccccaacgtc ctggggcctc tccactaata acggctactt
      121 ccaattgatt ggacgcgcca tcttgcctgc cttatgcata ttcagcggtg aactgaatat
      181 tcatgaacga ggcccgtccc gtccctccct cccttccccc ccccccggaa cccgctccgg
      241 aggacccgaa gggccccgcc ttcattaccg atgcgtagga caaaccattt tcccgatgtg
      301 tgtgggggga tactaatgag agactttagc tgaaaatgag cctgaactcc gaagctgagt
      361 aaaaatggcc taactttatc ctccgttctg taagtcctcg gtttgagtgc acgggaaacc
      421 cgaaaggagg acgacaggac caggaacatt ctcctcctcc tgtcgcgtca gaaagaacac
      481 ccaaccaggg agccggagcc ctagcgtcaa caactccgcc gcgcgcgctc cgtgtaggcc
      541 ggtgcgggcg gccccgtagc gcaagggagg gcgggaaagg aaggggcggg acacaagggc
      601 gaatctataa agggcgtcac tcagccagtt ctctcctcag aagcgccgag agcgcgaccg
      661 ggacggttgg agaagaaggt ggctcccgga agggggagag acaaactgcc gtaacctctg
      721 ccgttcagga tcactagtcc accatgggct acccctacga cgtgcccgac tacgccatcg
      781 aaggccgcca tatgctagcc gttaaaatgg cttctaactt tactcagttc gttctcgtcg
      841 acaatggcgg aactggcgac gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg
      901 aatggatcag ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga
      961 gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt
     1021 cgtacttaaa tatggaacta accattccaa ttttcgccac gaattccgac tgcgagctta
     1081 ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag
     1141 caaactccgg catctacgcg gatccagcca ccatggtgag caagggcgag gaggataaca
     1201 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1261 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1321 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1381 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     1441 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     1501 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     1561 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     1621 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     1681 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     1741 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     1801 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     1861 gcatggacga gctgtacaag ccaaaaaaga agagaaaggt ataaatcgat tctgcagtag
     1921 ctcgccggca gctgactagc tagagctgag aacttcaggg tgagtttggg gacccttgat
     1981 tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg
     2041 gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt
     2101 ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct
     2161 gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt
     2221 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata
     2281 ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga
     2341 atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac
     2401 accctggtca tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg
     2461 aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc
     2521 tctttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa
     2581 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     2641 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
     2701 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     2761 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     2821 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     2881 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     2941 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     3001 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     3061 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgagcgg
     3121 ccgcggtacc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     3181 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     3241 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3301 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3361 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3421 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3481 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3541 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3601 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3661 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3721 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     3781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4081 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4141 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4201 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4261 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4321 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aaacatgggg gatcatgtaa
     4381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5701 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5761 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5821 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     5881 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     5941 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     6001 gccgc
//
