LOCUS       V014551                 6017 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014551
VERSION     V014551
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             750..776
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             777..788
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1167..1184
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1185..1892
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     intron          1972..2544
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2548..2670
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    2741..2796
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     promoter        complement(3151..3169)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3176..3192)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3334..3789
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3815..3919
                     /label="AmpR promoter"
     misc_feature    4104..4483
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      4952..5540
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5828..5849
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5864..5894
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5902..5918
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5926..5942
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5963..5981
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tctagagtcg acctgcagcc caagcttggg ctgaacatcg agcgccaggg ctccgtaaag
       61 ctactagagc acaggcggtg ccccaacgtc ctggggcctc tccactaata acggctactt
      121 ccaattgatt ggacgcgcca tcttgcctgc cttatgcata ttcagcggtg aactgaatat
      181 tcatgaacga ggcccgtccc gtccctccct cccttccccc ccccccggaa cccgctccgg
      241 aggacccgaa gggccccgcc ttcattaccg atgcgtagga caaaccattt tcccgatgtg
      301 tgtgggggga tactaatgag agactttagc tgaaaatgag cctgaactcc gaagctgagt
      361 aaaaatggcc taactttatc ctccgttctg taagtcctcg gtttgagtgc acgggaaacc
      421 cgaaaggagg acgacaggac caggaacatt ctcctcctcc tgtcgcgtca gaaagaacac
      481 ccaaccaggg agccggagcc ctagcgtcaa caactccgcc gcgcgcgctc cgtgtaggcc
      541 ggtgcgggcg gccccgtagc gcaagggagg gcgggaaagg aaggggcggg acacaagggc
      601 gaatctataa agggcgtcac tcagccagtt ctctcctcag aagcgccgag agcgcgaccg
      661 ggacggttgg agaagaaggt ggctcccgga agggggagag acaaactgcc gtaacctctg
      721 ccgttcagga tcactagtcc accatgggct acccctacga cgtgcccgac tacgccatcg
      781 aaggccgcca tatgctagcc gttaaaatgg cttctaactt tactcagttc gttctcgtcg
      841 acaatggcgg aactggcgac gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg
      901 aatggatcag ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga
      961 gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt
     1021 cgtacttaaa tatggaacta accattccaa ttttcgccac gaattccgac tgcgagctta
     1081 ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag
     1141 caaactccgg catctacgcg gatccacacc atcaccatca ccatatggtg agcaagggcg
     1201 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1261 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1321 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1381 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1441 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1501 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     1561 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1621 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     1681 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     1741 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     1801 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     1861 actccaccgg cggcatggac gagctgtaca agccaaaaaa gaagagaaag gtataaatcg
     1921 attctgcagt agctcgccgg cagctgacta gctagagctg agaacttcag ggtgagtttg
     1981 gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc
     2041 aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca tggaccctca
     2101 tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc tcttattttc
     2161 ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg aatttttaaa
     2221 ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt ttttcaaggc
     2281 aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt tataattaaa
     2341 tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat tcttattggt
     2401 agaaacaact acaccctggt catcatcctg cctttctctt tatggttaca atgatataca
     2461 ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt
     2521 catgccttct tctctttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcat
     2581 cattttggca aagaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     2641 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     2701 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     2761 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     2821 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     2881 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     2941 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     3001 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     3061 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     3121 tccctcgagc ggccgcggta cccaattcgc cctatagtga gtcgtattac aattcactgg
     3181 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3241 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3301 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     3361 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3421 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3481 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3541 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3601 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3661 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3721 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     3781 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3901 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3961 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4021 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4081 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4141 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4201 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4261 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4321 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaaacatgg
     4381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5761 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5821 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5881 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5941 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggagctcc
     6001 accgcggtgg cggccgc
//
