LOCUS       V001621                13500 bp    DNA     circular SYN 21-DEC-2021
DEFINITION  Exported.
ACCESSION   V001621
VERSION     V001621
KEYWORDS    lentiSAMv2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13500)
  AUTHORS   Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham
            MD, Sanjana NE, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout and transcriptional activation
            screening.
  JOURNAL   Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016.
            Epub 2017 Mar 23.
   PUBMED   28333914
REFERENCE   2  (bases 1 to 13500)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13500)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1038/nprot.2017.016"; journalName: "Nat Protoc"; date:
            "2017-04"; volume: "12"; issue: "4"; pages: "828-863"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..459
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        507..554
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             573..944
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    1077..1210
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1247..1263)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1271..1287)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1295..1325)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1340..1361)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(1478..1495)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1649..2237)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2411..3268)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3269..3373)
                     /label="AmpR promoter"
     primer_bind     complement(3448..3467)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        3639..4018
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4019..4221
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             4236..4416
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4463..4588
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    5081..5314
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             5499..5543
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             5692..5733
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    5841..5958
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        6008..6248
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        6294..6312
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        6364..6382
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     promoter        6469..6680
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             6697..6744
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6745..10845
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             10900..10920
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             10945..11094
                     /label="VP64"
                     /note="tetrameric repeat of the minimal activation domain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
     CDS             11116..11169
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             11170..11565
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    11593..12181
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(12184..12200)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(12185..12201)
                     /label="pBluescriptKS"
                     /note="For pBluescript vector"
     LTR             12706..12886
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    12918..13142
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(13275..13294)
                     /label="F1ori-R"
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
       61 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
      121 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
      181 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      241 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      301 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      361 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      421 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
      481 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
      541 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
      601 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
      661 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
      721 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
      781 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
      841 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
      901 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
      961 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     1021 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     1081 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1141 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1201 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     1261 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1321 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1381 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1441 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1561 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2161 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2221 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2281 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2341 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2401 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2461 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2521 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2581 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2641 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2701 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2761 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2821 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2881 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2941 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3001 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3061 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3121 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3181 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3241 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3301 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3361 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     3421 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3481 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     3541 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     3601 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     3661 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3721 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3781 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     3841 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3901 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     3961 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4021 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     4081 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     4141 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     4201 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     4261 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4321 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4381 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     4441 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     4501 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     4561 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     4621 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     4681 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     4741 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     4801 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     4861 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     4921 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     4981 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     5041 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     5101 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     5161 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     5221 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     5281 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     5341 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     5401 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     5461 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     5521 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     5581 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     5641 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     5701 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     5761 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     5821 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     5881 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     5941 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     6001 agctagcgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     6061 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     6121 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     6181 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     6241 gaaaggacga aacaccggag acgggatacc gtctctgttt tagagctagg ccaacatgag
     6301 gatcacccat gtctgcaggg cctagcaagt taaaataagg ctagtccgtt atcaacttgg
     6361 ccaacatgag gatcacccat gtctgcaggg ccaagtggca ccgagtcggt gctttttttg
     6421 gatcctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     6481 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
     6541 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     6601 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     6661 ggtttgccgc cagaacacag gcgtacggcc accatgaaaa ggccggcggc cacgaaaaag
     6721 gccggccagg caaaaaagaa aaaggacaag aagtacagca tcggcctggc catcggcacc
     6781 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
     6841 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
     6901 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
     6961 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
     7021 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
     7081 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
     7141 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
     7201 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
     7261 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
     7321 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
     7381 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
     7441 aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
     7501 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
     7561 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
     7621 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
     7681 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
     7741 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
     7801 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
     7861 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
     7921 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
     7981 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
     8041 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
     8101 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
     8161 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
     8221 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
     8281 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
     8341 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
     8401 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
     8461 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
     8521 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
     8581 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
     8641 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
     8701 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
     8761 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
     8821 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
     8881 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
     8941 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
     9001 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
     9061 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
     9121 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
     9181 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
     9241 tccgactacg atgtggacca catcgtgcct cagagctttc tgaaggacga ctccatcgac
     9301 aacaaggtgc tgaccagaag cgacaaggcc cggggcaaga gcgacaacgt gccctccgaa
     9361 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
     9421 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
     9481 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
     9541 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
     9601 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
     9661 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
     9721 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
     9781 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
     9841 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
     9901 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
     9961 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
    10021 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
    10081 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
    10141 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
    10201 tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc
    10261 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
    10321 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
    10381 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
    10441 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
    10501 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
    10561 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
    10621 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
    10681 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
    10741 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
    10801 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacagcgc tggaggaggt
    10861 ggaagcggag gaggaggaag cggaggagga ggtagcggac ctaagaaaaa gaggaaggtg
    10921 gcggccgctg gatccggacg ggctgacgca ttggacgatt ttgatctgga tatgctggga
    10981 agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct tgatgacttt
    11041 gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat gctgattaac
    11101 tgtacaggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
    11161 cctggcccaa tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
    11221 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
    11281 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
    11341 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
    11401 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
    11461 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
    11521 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaaga attcgatatc
    11581 aagcttatcg gtaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
    11641 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
    11701 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
    11761 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
    11821 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
    11881 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
    11941 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
    12001 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
    12061 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
    12121 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    12181 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
    12241 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
    12301 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
    12361 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
    12421 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
    12481 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    12541 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
    12601 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
    12661 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
    12721 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    12781 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    12841 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
    12901 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
    12961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
    13021 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
    13081 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
    13141 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
    13201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    13261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    13321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    13381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    13441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
//
