LOCUS       Exported               11739 bp ds-DNA     circular SYN 20-DEC-2021
DEFINITION  lenti vector encoding the MS2-P65-HSF1 activator helper complex with
            a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This
            version has a higher virus titer..
ACCESSION   .
VERSION     .
KEYWORDS    lentiMPH v2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham
            MD, Sanjana NE, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout and transcriptional activation 
            screening.
  JOURNAL   Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. 
            Epub 2017 Mar 23.
  PUBMED    28333914
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2017.016"; journalName: "Nat Protoc"; date: 
            "2017-04"; volume: "12"; issue: "4"; pages: "828-863"
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        90..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     332..351
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        467..514
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             533..907
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1037..1158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1074..1093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1128..1147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1207..1223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1207..1223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1220..1242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1231..1247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1255..1285)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1300..1321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1438..1455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1609..2197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1689..1708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2368..3228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2991..3010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3229..3333)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3408..3427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3599..3978
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3980..4178
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4133..4153
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4196..4376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4423..4548
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5041..5274
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5459..5503
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5801..5918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5971..6559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6077..6088
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6486..6506
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6599..7618)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     CDS             complement(7622..7675)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(7691..8062)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             complement(8087..8629)
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             complement(8645..8665)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8720..9106)
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     regulatory      9106..9115
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(9122..10300)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9131..10069
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(9154..9174)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(10383..10399)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10384..10400)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10905..11085
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11111..11128)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11117..11341
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11387..11739,1..76)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11474..11493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11684..11705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
       61 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
      481 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
      541 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
      601 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
      661 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
      721 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
      781 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
      841 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
      901 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
      961 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1021 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1141 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     1201 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2041 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3361 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     3421 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     3481 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     3541 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     3601 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     3661 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     3721 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     3781 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3841 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3901 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3961 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4021 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4081 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4141 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     4201 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4261 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4321 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     4381 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     4441 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     4501 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     4561 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     4621 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     4681 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     4741 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     4801 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     4861 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     4921 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     4981 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     5041 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     5101 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     5161 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     5221 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     5281 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     5341 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     5401 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     5461 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     5521 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     5581 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     5641 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     5701 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     5761 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     5821 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5881 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     5941 gagatccagt ttggttaatt agctagcgat gcggggaggc ggcccaaagg gagatccgac
     6001 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
     6061 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     6121 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     6181 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     6241 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     6301 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     6361 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     6421 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     6481 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     6541 tgtaatccag aggttgatta ccgataagct tgatatcgaa ttctcattac tgcagatcct
     6601 ttgccctagg acgagtgctg gggcgtctgt ttccactatc ggcgagcact tccacacagc
     6661 catcggtcca gacggcagcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
     6721 cggaccggac gattgcgtcg cagcgaccct gagcccaagc tgcatcatcg aaattgccgt
     6781 caaccaagct ctgatagagt tggtcaagac caatgcggag catataagcc cggagccgag
     6841 gagatcctgc aagctcggga tgcctccgct cgaagtagcg ggtctgctgc tccatacaag
     6901 ccaaccaggg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatgg
     6961 cctcgctcca gtcaatgacg gctgttatgc ggccattgtc ggtcaggaca ttgttggagc
     7021 cgaaatcggc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
     7081 catcgagagc ctgggcgacg gaggcactga cggtgtcgtc catcacagtt tgccagtgat
     7141 acacatgggg atcagcaata gcgcagatga aatcgcgcca tgtagtgtat tgaccgattc
     7201 cttgaggtcc gaatgggccg aacccgctgg tctggctaag atcggcggca gcgatggcat
     7261 ccatggcctc ggcgacgggc tggagaacag cgggcagttc ggtttcaggc aggtcttgca
     7321 aggtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aactccccaa
     7381 tgtcaagcac ttcgggaata gggagagcgg cggatgcaaa gtgccgataa acatagcgat
     7441 ctttgtagaa accatcggcg cagctattca cccgcaggac atatccacgc cctcccacat
     7501 cgaagctgaa agcccgagat tcttcgccct cggagagctg catcaggtcg gagacgctgt
     7561 cgaacttttc gatcagaaac ttctcgacag aggtagcggt gagttcaggc tttttcatgg
     7621 ttgggccagg attctcctcg acgtcaccgc atgttagcag acttcctctg ccctctccac
     7681 tgcctgtaca ggagacagtg gggtccttgg ctttgggagg ctccgagcct gtcagcaggg
     7741 agatggtggg gtcctcggcg aagccgtccc cttcggagaa gtaggagccc tctcccagct
     7801 caaacagcac cggcaggtcg ttgctcccgg tgtccacgga gccggggtcc agcaggaaca
     7861 gcggctgcgc tgtgtagtgc accagctgct tccctgaatc cgggctgctg ttctctgcct
     7921 cgggaggcct ggggggctcc tggggagaca ggagctcttg gatactggcc aggctgctgt
     7981 caaggtcagg caggctcatg tcgggcacgg tcaccgaggg gctgaacagg tccagcaggg
     8041 cactggtgtc cacgctgaag ccgcttccac ctcctccctg cccactagag gaaatctgtg
     8101 acagcagggc actaaagtcc atatcagcga tgcttgagaa gtcttcatct ccggacagcc
     8161 cattaggcag gccgctggtt cccaggggag ttggagcggg gtcggggggc cgctggctgc
     8221 cggtcaccag ccgggtaatg gcttcggggt actccatcag cattggttcg gctgtactat
     8281 gagacatgga cacgccctga ttcagcagct gctgaaactc agagttgtcc acggaggcca
     8341 gatctgtgaa cactccggga tcggtgctgt tccccagcag agctcccagg tcctcatcag
     8401 cgtcgaactg caggtgcagc agagcttcac tcagagtccc ctcgccggcc tgtgtagact
     8461 tgggcactgg agcgctcagt gactggggtg gtcctggggt cagcacaggg gctggagcag
     8521 gtggctgggc cagaggcacc atagcactag agggcaccat agtctgggcc agcactggag
     8581 cggagctagg ggccagagcc agggcctggt tgctgatctg ccctgaaggg gatccagcgg
     8641 ccgccacctt cctctttttc ttaggtccgc tacctcctcc tccgcttcct cctcctccgc
     8701 ttccacctcc tccagcgctg tagatacctg agttagcggc gatggcggaa gggataggat
     8761 taccgtcttt gaggagcccc tgcattgcct tcacgatgag ttcacagtca gaattggtag
     8821 cgaaaattgg gatagtgagc tccatgttca ggtaggacct ccaagcggcg acaggcagtt
     8881 cgactccgcc cactgtctgg gtagccactt tggggacctc caccttgatg gtatactttc
     8941 tcttctgggc actagactgc ctgacgctgc atgtcacctt gtaggcctgg ctccgtgagt
     9001 tggagctgat ccactctgcc accccattag cgaaattaga aggagccact gtcacatccc
     9061 ctgtcccacc attgtccacg agcacgaact gagtaaagtt tgaagccatg gtggccgtac
     9121 gtcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga
     9181 accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag tgccaagctg
     9241 gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa aacccctccc
     9301 cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg
     9361 cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc cttttccttt
     9421 gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt ccttcatttt gagctccctg
     9481 cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac cgggccagcc
     9541 ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga gcaggccggc
     9601 cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg
     9661 aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa accgaaatac
     9721 cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag
     9781 caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga caggcgcgaa
     9841 ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca actcaagcac
     9901 gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa
     9961 gctcgggatc aagaatcacg tactgcagcc aggtggaagt aattcaaggc acgcaagggc
    10021 cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc tgtgttctgg
    10081 cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc
    10141 ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc
    10201 gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg
    10261 tgggcgatgt gcgctctgcc cactgacggg caccggagcc aattcccact cctttcaaga
    10321 cctagaaggt ccattagctg caaagattcc tctctgttta aaactttatc catctttgca
    10381 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
    10441 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
    10501 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    10561 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
    10621 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
    10681 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
    10741 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
    10801 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
    10861 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
    10921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    11041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
    11101 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
    11161 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
    11221 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
    11281 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
    11341 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
    11401 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    11461 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    11521 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    11581 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    11641 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    11701 actggaacaa cactcaaccc tatctcggtc tattctttt
//
