LOCUS lentiCas9-Blast. 12859 bp DNA circular SYN 01-JAN-1980 DEFINITION Zhang lab lentiviral vector with a blasticidin resistance marker, also known as pLentiCas9-Blast, for expressing Cas9. Use together with lentiGuide-Puro. ACCESSION . VERSION . KEYWORDS lentiCas9-Blast. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12859) AUTHORS Sanjana NE, Shalem O, Zhang F. TITLE Improved vectors and genome-wide libraries for CRISPR screening. JOURNAL Nat. Methods 2014;11:783-4. PUBMED 25075903 REFERENCE 2 (bases 1 to 12859) AUTHORS Zhang Lab / Addgene #52962 TITLE Direct Submission REFERENCE 3 (bases 1 to 12859) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Methods"; date: "2014"; volume: "11"; pages: "783-4" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..12859 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 618..820 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 2291..2332 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2650..2861 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" CDS 2886..6989 /label=Cas9 /note="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" CDS 6990..7037 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=bipartite nuclear localization signal from nucl /note="nucleoplasmin NLS" /translation="KRPAATKKAGQAKKKK" CDS 7038..7061 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" CDS 7071..7127 /label=P2A /note="2A peptide from porcine teschovirus-1 polyprotein" CDS 7128..7523 /label=BSD /note="blasticidin S deaminase" misc_feature 7551..8139 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 8664..8844 /label=3' LTR (truncated) /note="3' LTR (truncated)" /note="truncated long terminal repeat (LTR) from HIV-1" polyA_signal 8876..9100 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 9146..9574 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 9588..9917 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 9965..10012 /note="EM7 promoter" /note="synthetic bacterial promoter " CDS 10031..10402 /label=BleoR /note="antibiotic-binding protein" polyA_signal 10535..10668 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(10753..10783) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(10798..10819) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(11107..11695) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11869..12726) /label=AmpR /note="beta-lactamase" promoter complement(12727..12831) /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg 2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagagcgctg 2881 ccaccatgga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg 2941 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg 3001 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag 3061 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga 3121 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc 3181 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct 3241 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga 3301 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg 3361 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca 3421 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa 3481 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga 3541 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg 3601 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg 3661 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc 3721 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg 3781 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg 3841 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg 3901 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct 3961 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca 4021 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc 4081 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc 4141 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa 4201 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa 4261 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg 4321 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg 4381 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca 4441 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct 4501 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag 4561 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg 4621 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga 4681 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata 4741 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct 4801 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct 4861 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa 4921 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg 4981 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata 5041 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc 5101 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca 5161 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg 5221 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac 5281 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg 5341 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg 5401 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca 5461 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga 5521 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca 5581 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga 5641 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga 5701 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga 5761 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca 5821 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca 5881 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc 5941 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct 6001 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga 6061 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg 6121 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg 6181 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc 6241 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg 6301 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga 6361 gtgtgaaaga gctactgggg atcaccatca tggaaagaag cagcttcgag aagaatccca 6421 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc 6481 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg 6541 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg 6601 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg 6661 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga 6721 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg 6781 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg 6841 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca 6901 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac 6961 ggatcgacct gtctcagctg ggaggcgaca agcgacctgc cgccacaaag aaggctggac 7021 aggctaagaa gaagaaagat tacaaagacg atgacgataa gggatccggc gcaacaaact 7081 tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gccaagcctt 7141 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca 7201 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg 7261 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg 7321 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg 7381 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca 7441 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc 7501 cctctggtta tgtgtgggag ggctaagaat tcgatatcaa gcttatcggt aatcaacctc 7561 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 7621 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 7681 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 7741 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 7801 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 7861 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 7921 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg 7981 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 8041 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 8101 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag 8161 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg 8221 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt 8281 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 8341 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc 8401 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc 8461 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag 8521 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc 8581 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc 8641 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc 8701 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 8761 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 8821 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg 8881 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 8941 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 9001 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 9061 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 9121 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 9181 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 9241 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 9301 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 9361 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 9421 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 9481 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 9541 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 9601 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 9661 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 9721 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 9781 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 9841 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 9901 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc 9961 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag 10021 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc 10081 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga 10141 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt 10201 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga 10261 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat 10321 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca 10381 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt 10441 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 10501 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 10561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 10621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc 10681 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 10741 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 10801 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 10861 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 10921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 10981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 11041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 11101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 11161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 11221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 11281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 11341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 11401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 11461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 11521 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 11581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 11641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 11701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 11761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 11821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 11881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 11941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 12001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 12061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 12121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 12181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 12241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 12301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 12361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 12421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 12481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 12541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 12601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 12661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 12721 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 12781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 12841 ccgaaaagtg ccacctgac //