LOCUS       Exported               15068 bp DNA     circular SYN 07-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    lentiCRISPRv2 neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15068)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..15068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    878..899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        914..944
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    952..968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     976..992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1041..1162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1292..1666)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1685..1732)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1780..2109)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1794..1929
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2123..2551)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    2597..2821
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             2853..3086
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3158..3746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3264..3275
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(3762..4553)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4554..4610)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4620..4643)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(4644..4691)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(4692..8795)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     regulatory      8792..8801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8820..9031)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(9093..9168)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(11058..11298)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    11349..11466
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11764..11808)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11993..12226
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12719..12844
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             12891..13071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(13089..13287)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        13289..13668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13934..14038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14039..14899
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      901 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      961 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg
     1021 tcgacggtat acagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     1081 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     1141 tataagctgc aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct
     1201 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     1261 cggcggtgga atcgaaatct cgtagcacgt gtcagtcctg ctcctcggcc acgaagtgca
     1321 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
     1381 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
     1441 cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct
     1501 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
     1561 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
     1621 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatggtt tagttcctca
     1681 ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtca acacgtgctg
     1741 atcagatccg aaaatggata tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc
     1801 aaaaaagcct cctcactact tctggaatag ctcagaggca gaggcggcct cggcctctgc
     1861 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     1921 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     1981 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     2041 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     2101 ctgacacaca ttccacagaa ttaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2161 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2221 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2281 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2341 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     2401 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     2461 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     2521 gccgcgctta atgcgccgct acagggcgcg tggggatacc ccctagagcc ccagctggtt
     2581 ctttccgcct cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc
     2641 caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc
     2701 agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc
     2761 agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca
     2821 gtcgaggctg atcagcgggt ttaaacgggc cctgctagag attttccaca ctgactaaaa
     2881 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     2941 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     3001 ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct
     3061 tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc
     3121 tacagctgcc ttgtaagtca ttggtcttaa agtcgacgcg gggaggcggc ccaaagggag
     3181 atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg
     3241 ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg
     3301 cgcagaatcc aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac
     3361 aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag
     3421 gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc
     3481 tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc
     3541 acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta
     3601 tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa
     3661 gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca
     3721 caaattttgt aatccagagg ttgattgtcg acttaacgcg ttcagaagaa ctcgtcaaga
     3781 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     3841 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     3901 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     3961 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     4021 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     4081 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     4141 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     4201 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     4261 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     4321 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     4381 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     4441 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     4501 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcggtcca
     4561 ggattctctt cgacatctcc ggcttgtttc agcagagaga agtttgttgc gccggatccc
     4621 ttatcgtcat cgtctttgta atctttcttc ttcttagcct gtccagcctt ctttgtggcg
     4681 gcaggtcgct tgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg
     4741 atgctctggt ggatcagggt ggcgtccagc acctctttgg tgctggtgta cctcttccgg
     4801 tcgatggtgg tgtcaaagta cttgaaggcg gcaggggctc ccagattggt cagggtaaac
     4861 aggtggatga tattctcggc ctgctctctg atgggcttat cccggtgctt gttgtaggcg
     4921 gacagcactt tgtccagatt agcgtcggcc aggatcactc tcttggagaa ctcgctgatc
     4981 tgctcgatga tctcgtccag gtagtgcttg tgctgttcca caaacagctg tttctgctca
     5041 ttatcctcgg gggagccctt cagcttctca tagtggctgg ccaggtacag gaagttcaca
     5101 tatttggagg gcagggccag ttcgtttccc ttctgcagtt cgccggcaga ggccagcatt
     5161 ctcttccggc cgttttccag ctcgaacagg gagtacttag gcagcttgat gatcaggtcc
     5221 tttttcactt ctttgtagcc cttggcttcc agaaagtcga tgggattctt ctcgaagctg
     5281 cttctttcca tgatggtgat ccccagcagc tctttcacac tcttcagttt cttggacttg
     5341 cccttttcca ctttggccac caccagcaca gaataggcca cggtggggct gtcgaagccg
     5401 ccgtacttct tagggtccca gtccttcttt ctggcgatca gcttatcgct gttcctcttg
     5461 ggcaggatag actctttgct gaagccgcct gtctgcacct cggtcttttt cacgatattc
     5521 acttggggca tgctcagcac tttccgcacg gtggcaaaat cccggccctt atcccacacg
     5581 atctccccgg tttcgccgtt tgtctcgatc agaggccgct tccggatctc gccgttggcc
     5641 agggtaatct cggtcttgaa aaagttcatg atgttgctgt agaagaagta cttggcggta
     5701 gccttgccga tttcctgctc gctcttggcg atcatcttcc gcacgtcgta caccttgtag
     5761 tcgccgtaca cgaactcgct ttccagctta gggtactttt tgatcagggc ggttcccacg
     5821 acggcgttca ggtaggcgtc gtgggcgtgg tggtagttgt tgatctcgcg cactttgtaa
     5881 aactggaaat ccttccggaa atcggacacc agcttggact tcagggtgat cactttcact
     5941 tcccggatca gcttgtcatt ctcgtcgtac ttagtgttca tccgggagtc caggatctgt
     6001 gccacgtgct ttgtgatctg ccgggtttcc accagctgtc tcttgatgaa gccggcctta
     6061 tccagttcgc tcaggccgcc tctctcggcc ttggtcagat tgtcgaactt tctctgggta
     6121 atcagcttgg cgttcagcag ctgccgccag tagttcttca tcttcttcac gacctcttcg
     6181 gagggcacgt tgtcgctctt gccccggttc ttgtcgcttc tggtcagcac cttgttgtcg
     6241 atggagtcgt ccttcagaaa gctctgaggc acgatatggt ccacatcgta gtcggacagc
     6301 cggttgatgt ccagttcctg gtccacgtac atatcccgcc cattctgcag gtagtacagg
     6361 tacagcttct cgttctgcag ctgggtgttt tccacggggt gttctttcag gatctggctg
     6421 cccagctctt tgatgccctc ttcgatccgc ttcattctct cgcggctgtt cttctgtccc
     6481 ttctgggtgg tctggttctc tctggccatt tcgatcacga tgttctcggg cttgtgccgg
     6541 cccatcactt tcacgagctc gtccaccacc ttcactgtct gcaggatgcc cttcttaatg
     6601 gcggggctgc cggccagatt ggcaatgtgc tcgtgcaggc tatcgccctg gccggacacc
     6661 tgggctttct ggatgtcctc tttaaaggtc aggctgtcgt cgtggatcag ctgcatgaag
     6721 tttctgttgg cgaagccgtc ggacttcagg aaatccagga ttgtcttgcc ggactgcttg
     6781 tcccggatgc cgttgatcag cttccggctc agcctgcccc agccggtgta tctccgccgc
     6841 ttcagctgct tcatcacttt gtcgtcgaac aggtgggcat aggttttcag ccgttcctcg
     6901 atcatctctc tgtcctcaaa cagtgtcagg gtcagcacga tatcttccag aatgtcctcg
     6961 ttttcctcat tgtccaggaa gtccttgtcc ttgataattt tcagcagatc gtggtatgtg
     7021 cccagggagg cgttgaaccg atcttccacg ccggagattt ccacggagtc gaagcactcg
     7081 attttcttga agtagtcctc tttcagctgc ttcacggtca ctttccggtt ggtcttgaac
     7141 agcaggtcca cgatggcctt tttctgctcg ccgctcagga aggcgggctt tctcattccc
     7201 tcggtcacgt atttcacttt ggtcagctcg ttatacacgg tgaagtactc gtacagcagg
     7261 ctgtgcttgg gcagcacctt ctcgttgggc aggttcttat cgaagttggt catccgctcg
     7321 atgaagctct gggcggaagc gcccttgtcc accacttcct cgaagttcca gggggtgatg
     7381 gtttcctcgc tctttctggt catccaggcg aatctgctgt ttcccctggc cagagggccc
     7441 acgtagtagg ggatgcggaa ggtcaggatc ttctcgatct tttcccggtt gtccttcagg
     7501 aatgggtaaa aatcttcctg ccgccgcaga atggcgtgca gctctcccag gtggatctgg
     7561 tgggggatgc tgccgttgtc gaaggtccgc tgcttccgca gcaggtcctc tctgttcagc
     7621 ttcacgagca gttcctcggt gccgtccatc ttttccagga tgggcttgat gaacttgtag
     7681 aactcttcct ggctggctcc gccgtcaatg tagccggcgt agccgttctt gctctggtcg
     7741 aagaaaatct ctttgtactt ctcaggcagc tgctgccgca cgagagcttt cagcagggtc
     7801 aggtcctggt ggtgctcgtc gtatctcttg atcatagagg cgctcagggg ggccttggtg
     7861 atctcggtgt tcactctcag gatgtcgctc agcaggatgg cgtcggacag gttcttggcg
     7921 gccagaaaca ggtcggcgta ctggtcgccg atctgggcca gcaggttgtc caggtcgtcg
     7981 tcgtaggtgt ccttgctcag ctgcagtttg gcatcctcgg ccaggtcgaa gttgctcttg
     8041 aagttggggg tcaggcccag gctcagggca atcaggtttc cgaacaggcc attcttcttc
     8101 tcgccgggca gctgggcgat cagattttcc agccgtctgc tcttgctcag tctggcagac
     8161 aggatggcct tggcgtccac gccgctggcg ttgatggggt tttcctcgaa cagctggttg
     8221 taggtctgca ccagctggat gaacagcttg tccacgtcgc tgttgtcggg gttcaggtcg
     8281 ccctcgatca ggaagtggcc ccggaacttg atcatgtggg ccagggccag atagatcagc
     8341 cgcaggtcgg ccttgtcggt gctgtccacc agtttctttc tcaggtggta gatggtgggg
     8401 tacttctcgt ggtaggccac ctcgtccacg atgttgccga agatggggtg ccgctcgtgc
     8461 ttcttatcct cttccaccag gaaggactct tccagtctgt ggaagaagct gtcgtccacc
     8521 ttggccatct cgttgctgaa gatctcttgc agatagcaga tccggttctt ccgtctggtg
     8581 tatcttcttc tggcggttct cttcagccgg gtggcctcgg ctgtttcgcc gctgtcgaac
     8641 agcagggctc cgatcaggtt cttcttgatg ctgtgccggt cggtgttgcc cagcaccttg
     8701 aatttcttgc tgggcacctt gtactcgtcg gtgatcacgg cccagcccac agagttggtg
     8761 ccgatgtcca ggccgatgct gtacttcttg tccatggtgg cagcgctcta gaaccggtcc
     8821 tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata
     8881 tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag
     8941 tttaccccgc gccaccttct ctaggcaccg gatcaattgc cgacccctcc ccccaacttc
     9001 tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc aattcccact
     9061 cctttcaaga cctagctagc gaattcaaaa aagcaccgac tcggtgccac tttttcaagt
     9121 tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacag agacgtacaa
     9181 aaaagagcaa gaagctaaaa aagatttaaa aattattttt agcgcagtta atggaacagg
     9241 aactaaattt accccaaaaa tattacgtga atcaggatat aacgttattg aggttgaaga
     9301 gcatgcattt gaagatgaaa catttaaaaa tgttgtaaat ccaaatccag aatttgatcc
     9361 tgcatgaaaa ataccgcttg aatatggtat taaacatgat gcagatatta ttattatgaa
     9421 tgacccagat gctgacagat ttggaatggc aataaaacat gatggtcatt ttgtaagatt
     9481 agatggaaat caaacaggac caattttaat tgattgaaaa ttatcaaatc taaaacgctt
     9541 aaatagcatt ccaaaaaatc cggctctata ttcaagtttt gtaacaagtg atttgggtga
     9601 tagaatcgct catgaaaaat atggagttaa tattgtaaaa actttaactg gatttaaatg
     9661 aatgggtaga gaaattgcta aagaagaaga taacggatta aattttgttt ttgcttatga
     9721 agaaagttat ggatatgtaa ttgatgactc agctagagat aaagatggaa tacaagcttc
     9781 tatattaata gcagaggctg cttgatttta taaaaaacaa aataaaacat tagtagacta
     9841 tttagaagat ttatttaaag aaatgggtgc atattacact ttcactttaa acttgaattt
     9901 taaaccagaa gaaaagaaat taaaaattga accattaatg aaatcattga gagcaacacc
     9961 cttaactcaa attgctggac ttaaagttgt taatgttgaa gactacatcg atggaatgta
    10021 taatatgcca ggacaagact tactaaaatt ttatttagaa gataagtcat gatttgctgt
    10081 tcgcccaagt ggaactgaac ctaaactaaa aatttatttt ataggtgttg gtgaatctgt
    10141 tcaaaacgct aaagttaaag tagacgaaat tattaaagaa ttaaaattaa aaatgaatat
    10201 ataggagaaa aaatgaaact aaacaaatat atagatcaca cattattaaa acaagatgct
    10261 acgaaagctg aaattaaaca attatgtgat gaagcaattg aatttgattt tgcaacagtt
    10321 tgtgttaatt catattgaac aagctattgt aaagaattat taaaaggcac aaatgtagga
    10381 ataacaaatg ttgtaggttt tcctctaggt gcatgcacaa cagctacaaa agcattcgaa
    10441 gtttctgaag caattaaaga tggtgcaaca gaaattgata tggtattaaa tattggtgca
    10501 ttaaaagaca aaaattatga attagtttta gaagacatga aagctgtaaa aaaagcagct
    10561 ggatcacatg ttgttaaatg tattatggaa aattgtttat taacaaaaga agaaatcatg
    10621 aaagcttgtg aaatagctgt tgaagctgga ttagaatttg ttaaaacatc aacaggattt
    10681 tcaaaatcag gtgcaacatt tgaagatgtt aaactaatga agtcagttgt taaagacaat
    10741 gctttagtta aagcagctgg tggagttaga acatttgaag atgctcaaaa aatgattgaa
    10801 gcaggagctg accgcttagg aacaagtggt ggagtagcta ttattaaagg tgaagaaaac
    10861 aacgcgagtt actaaaacta gcgttttttt attttgctca tttttattaa aagtttgcaa
    10921 aaaggaacat aaaaattcta attattgata ctaaagttat taaaaagaag attttggttg
    10981 attttataaa ggtcatagaa tataatattt tagcatgtgt attttgtgtg ctcatttaca
    11041 accgtctccg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt
    11101 tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta
    11161 cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt
    11221 caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat
    11281 catgggaaat aggccctcgg taccttaatt aaccaaactg gatctctgct gtccctgtaa
    11341 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
    11401 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
    11461 ttaaaattgt ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc
    11521 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
    11581 gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
    11641 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
    11701 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
    11761 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
    11821 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
    11881 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
    11941 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
    12001 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
    12061 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
    12121 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
    12181 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
    12241 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
    12301 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc
    12361 aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct
    12421 ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga
    12481 gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt
    12541 gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg
    12601 cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc
    12661 cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga
    12721 cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca
    12781 ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag
    12841 agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat
    12901 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
    12961 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
    13021 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaggc
    13081 aaaacgcgct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
    13141 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
    13201 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
    13261 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
    13321 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
    13381 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
    13441 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
    13501 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
    13561 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
    13621 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
    13681 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
    13741 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
    13801 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
    13861 tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt
    13921 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    13981 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    14041 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    14101 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    14161 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    14221 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    14281 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    14341 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    14401 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    14461 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    14521 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    14581 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    14641 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    14701 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    14761 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    14821 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    14881 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    14941 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    15001 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    15061 aggatctt
//
