LOCUS       Exported               14801 bp DNA     circular SYN 19-JUN-2025
DEFINITION  Exported.
ACCESSION   V012328
VERSION     .
KEYWORDS    lentiCRISPR v2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14801)
  AUTHORS   Zhang Lab / Addgene #52961
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 14801)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14801)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             2291..2332
                     /label=Protein Tat
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2608..2848
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2853..4737
                     /label=filler
                     /note="filler"
                     /note="digest with BsmBI to replace the filler with an
                     sgRNA   "
     misc_RNA        4738..4813
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      4814..4819
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     promoter        4875..5086
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             5111..9214
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             9215..9262
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=bipartite nuclear localization signal from nucl
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9263..9286
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             9296..9352
                     /label=P2A
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             9353..9946
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     CDS             complement(10436..10447)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     LTR             10625..10858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10890..11114
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11160..11588
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        11641..11851
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11907..11954
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             11973..12344
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    12477..12610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(12695..12725)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12740..12761)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(13049..13637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13811..14668)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(14669..14773)
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac
     2881 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct
     2941 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat
     3001 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt
     3061 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg
     3121 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac
     3181 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt
     3241 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt
     3301 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac
     3361 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa
     3421 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt
     3481 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc
     3541 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc
     3601 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa
     3661 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat
     3721 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca
     3781 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac
     3841 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg
     3901 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat
     3961 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa
     4021 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt
     4081 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca
     4141 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca
     4201 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt
     4261 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt
     4321 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt
     4381 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga
     4441 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata
     4501 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga
     4561 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata
     4621 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact
     4681 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt
     4741 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4801 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     4861 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4921 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4981 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5041 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag
     5101 cgctgccacc atggacaaga agtacagcat cggcctggac atcggcacca actctgtggg
     5161 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
     5221 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
     5281 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
     5341 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
     5401 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
     5461 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     5521 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     5581 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     5641 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     5701 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     5761 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     5821 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     5881 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     5941 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     6001 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     6061 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     6121 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     6181 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
     6241 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     6301 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     6361 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     6421 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     6481 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     6541 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
     6601 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     6661 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     6721 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
     6781 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     6841 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     6901 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     6961 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     7021 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     7081 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     7141 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     7201 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     7261 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     7321 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     7381 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     7441 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     7501 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     7561 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     7621 tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
     7681 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     7741 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     7801 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     7861 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     7921 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     7981 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     8041 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     8101 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     8161 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     8221 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     8281 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     8341 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     8401 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     8461 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     8521 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     8581 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     8641 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     8701 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     8761 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     8821 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     8881 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     8941 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     9001 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     9061 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     9121 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     9181 gacacggatc gacctgtctc agctgggagg cgacaagcga cctgccgcca caaagaaggc
     9241 tggacaggct aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac
     9301 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgaccgagta
     9361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     9421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     9541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     9601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     9661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     9721 cctggccacc gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     9781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     9841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt
     9961 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
    10021 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
    10081 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
    10141 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
    10201 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
    10261 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
    10321 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
    10381 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
    10441 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
    10501 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
    10561 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    10621 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
    10681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
    10861 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    10921 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    10981 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    11041 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    11101 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
    11161 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    11221 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    11281 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    11341 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    11401 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    11461 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    11521 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    11581 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
    11641 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    11701 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    11761 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
    11821 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
    11881 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
    11941 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
    12001 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
    12061 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
    12121 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
    12181 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
    12241 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
    12301 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
    12361 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    12421 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    12481 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    12541 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    12601 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    12661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    12721 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    12781 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    12841 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    12901 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12961 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    13021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    13081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    13141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    13201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    13261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    13321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    13381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    13441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    13501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    13561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    13621 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    13681 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    13741 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    13801 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    13861 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    13921 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    13981 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    14041 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    14101 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    14161 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    14221 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    14281 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    14341 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    14401 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    14461 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    14521 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    14581 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    14641 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    14701 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    14761 taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
