LOCUS       .                      12478 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2669..2880
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     CDS             2905..7008
                     /label="Cas9"
                     /note="S. pyogenes Cas9, codon optimized for C. elegans
                     with synthetic introns"
     CDS             7057..7080
                     /label="FLAG"
                     /note="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             7090..7146
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             7147..7542
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    7570..8158
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8230..8463
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8495..8719
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8765..9193
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9207..9536
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        9584..9631
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             9650..10021
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    10154..10287
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(10372..10402)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10417..10438)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10726..11314)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11488..12345)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(12346..12450)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccctc gagtgtacag ctagctaggt cttgaaagga
     2641 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2701 aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac
     2761 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2821 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2881 gaccggttct agagcgctgc caccatggac aagaagtaca gcatcggcct ggacatcggc
     2941 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
     3001 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
     3061 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
     3121 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
     3181 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
     3241 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
     3301 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
     3361 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
     3421 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
     3481 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
     3541 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
     3601 aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc
     3661 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac
     3721 gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc
     3781 gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc
     3841 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg
     3901 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc
     3961 gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc
     4021 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag
     4081 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac
     4141 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc
     4201 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg
     4261 ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc
     4321 atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc
     4381 gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc
     4441 ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag
     4501 ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg
     4561 ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc
     4621 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc
     4681 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac
     4741 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc
     4801 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag
     4861 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac
     4921 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac
     4981 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag
     5041 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc
     5101 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc
     5161 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag
     5221 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc
     5281 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac
     5341 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg
     5401 ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc
     5461 gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc
     5521 gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt
     5581 acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat
     5641 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca
     5701 cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa
     5761 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt
     5821 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc
     5881 gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac
     5941 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct
     6001 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     6061 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc
     6121 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     6181 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc
     6241 aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc
     6301 ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc
     6361 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc
     6421 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag
     6481 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
     6541 atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat
     6601 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
     6661 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
     6721 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
     6781 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
     6841 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
     6901 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
     6961 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacaa gcgacctgcc
     7021 gccacaaaga aggctggaca ggctaagaag aagaaagatt acaaagacga tgacgataag
     7081 ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct
     7141 ggaccgatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     7201 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     7261 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     7321 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     7381 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     7441 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     7501 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagaatt cgatatcaag
     7561 cttatcggta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7621 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7681 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7741 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7801 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7861 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7921 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7981 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8041 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8101 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     8161 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8221 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8281 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8341 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8401 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8461 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     8521 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     8581 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     8641 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     8701 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     8761 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8821 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8881 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8941 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9001 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9061 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9121 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9181 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     9241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     9301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9361 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     9481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9541 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     9601 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     9661 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     9721 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     9781 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     9841 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     9901 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     9961 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
    10021 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10081 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10141 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10261 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    10321 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10381 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10441 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10501 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10561 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10621 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10681 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10741 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10801 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10861 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10921 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10981 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11041 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11101 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11161 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11221 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11281 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11341 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11401 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11461 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11521 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11581 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11641 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11701 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11761 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11821 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11881 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11941 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12001 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12061 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12121 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12181 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12241 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12301 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12361 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12421 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
