LOCUS       V017403                13818 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017403
VERSION     V017403
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13818)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..583
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             598..778
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1443..1676
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1861..1905
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2054..2095
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2203..2320
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2365..2812
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     protein_bind    complement(2825..2858)
                     /label="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
                     compatible with each other, but incompatible with loxP or
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2909..2942)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(4430..4450)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             complement(4457..4522)
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     protein_bind    4532..4565
                     /label="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
                     compatible with each other, but incompatible with loxP or
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4616..4649
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4914..7412
                     /label="Cas9(C)"
                     /note="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
     CDS             7521..7715
                     /label="KRAB"
                     /note="Kruppel-associated box (KRAB) transcriptional
                     repression domain from the human zinc finger protein ZNF10
                     (Margolin et al., 1994)"
     CDS             7749..7769
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             7779..8636
                     /label="MeCP2"
                     /note="transcriptional repression domain from rat
                     methyl-CpG-binding protein 2 (Nan et al., 1997)"
     misc_feature    8673..9261
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             9333..9566
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9598..9822
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        10310..10639
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        10687..10734
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             10753..11124
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    11257..11390
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(11475..11505)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11520..11541)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11829..12417)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12591..13448)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13449..13553)
                     /label="AmpR promoter"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaagtgca agtgggtttt aggaccagga tgaggcgggg
     2401 tgggggtgcc tacctgacga ccgaccccga cccactggac aagcacccaa cccccattcc
     2461 ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc gaggcgcgtg
     2521 cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca
     2581 ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct
     2641 tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc accacgcgag
     2701 gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct
     2761 gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc agaccggtgg
     2821 taccataact tcgtatagga tactttatac gaagttatgc agaatggtag ctggattgta
     2881 gctgctatta gcaatatgaa acctcttaat aacttcgtat agcatacatt atacgaagtt
     2941 atactaccca ggctagcccg gtgccaccat caccggatcc gggacctgaa ataaaagaca
     3001 aaaagactaa acttacccag gcgaatctgc tgtttcccct ggccagaggg cccacgtagt
     3061 aggggatgcg gaaggtcagg atcttctcga tcttttcccg gttgtccttc aggaatgggt
     3121 aaaaatcttc ctgccgccgc agaatggcgt gcagctctcc caggtggatc tggtggggga
     3181 tgctgccgtt gtcgaaggtc cgctgcttcc gcagcaggtc ctctctgttc agcttcacga
     3241 gcagttcctc ggtgccgtcc atcttttcca ggatgggctt gatgaacttg tagaactctt
     3301 cctggctggc tccgccgtca atgtagccgg cgtagccgtt cttgctctgg tcgaagaaaa
     3361 tctctttgta cttctcaggc agctgctgcc gcacgagagc tttcagcagg gtcaggtcct
     3421 ggtggtgctc gtcgtatctc ttgatcatag aggcgctcag gggggccttg gtgatctcgg
     3481 tgttcactct caggatgtcg ctcagcagga tggcgtcgga caggttcttg gcggccagaa
     3541 acaggtcggc gtactggtcg ccgatctggg ccagcaggtt gtccaggtcg tcgtcgtagg
     3601 tgtccttgct cagctgcagt ttggcatcct cggccaggtc gaagttgctc ttgaagttgg
     3661 gggtcaggcc caggctcagg gcaatcaggt tgccgaacag gccattcttc ttctcgccgg
     3721 gcagctgggc gatcagattt tccagccgtc tgctcttgct cagtctggca gacaggatgg
     3781 ccttggcgtc cacgccgctg gcgttgatgg ggttttcctc gaacagctgg ttgtaggtct
     3841 gcaccagctg gatgaacagc ttgtccacgt cgctgttgtc ggggttcagg tcgccctcga
     3901 tcaggaagtg gccccggaac ttgatcatgt gggccagggc cagatagatc agccgcaggt
     3961 cggccttgtc ggtgctgtcc accagtttct ttctcaggtg gtagatggtg gggtacttct
     4021 cgtggtaggc cacctcgtcc acgatgttgc cgaagatggg gtgccgctcg tgcttcttat
     4081 cctcttccac caggaaggac tcttccagtc tgtggaagaa gctgtcgtcc accttggcca
     4141 tctcgttgct gaagatctct tgcagatagc agatccggtt cttccgtctg gtgtatcttc
     4201 ttctggcggt tctcttcagc cgggtggcct cggctgtttc gccgctgtcg aacagcaggg
     4261 ctccgatcag gttcttcttg atgctgtgcc ggtcggtgtt gcccagcacc ttgaatttct
     4321 tgctgggcac cttgtactcg tcggtgatca cggcccagcc cacagagttg gtgccgatgg
     4381 ccaggccgat gctgtacttc ttgtcggctg ctgggactcc gtggataccg accttccgct
     4441 tcttctttgg ggccatctta tcgtcatcgt ctttgtaatc aatatcatga tccttgtagt
     4501 ctccgtcgtg gtccttatag tccatggtgg cataacttcg tataaagtat cctatacgaa
     4561 gttatttgcc ttaacccaga aattatcact gttattcttt agaatggtgc aaagaataac
     4621 ttcgtataat gtatgctata cgaagttatg tggtgcaaat caaagaactg ctcctcagtg
     4681 gatgttgcct ttacttctag atgaccagaa agagcgagga aaccatcacc ccctggaact
     4741 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact
     4801 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     4861 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     4921 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga
     4981 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     5041 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     5101 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     5161 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     5221 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     5281 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     5341 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
     5401 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     5461 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     5521 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     5581 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     5641 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     5701 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     5761 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     5821 tggacgctat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga
     5881 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     5941 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg
     6001 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     6061 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     6121 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc
     6181 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
     6241 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
     6301 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
     6361 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
     6421 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
     6481 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc
     6541 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga
     6601 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata
     6661 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
     6721 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
     6781 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
     6841 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
     6901 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
     6961 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
     7021 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
     7081 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca
     7141 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
     7201 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
     7261 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
     7321 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
     7381 cacggatcga cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg
     7441 gacaggccaa aaagaaaaag agtggtggag gaagtggcgg gtcagggtcg atggacgcga
     7501 aatcacttac ggcatggtcg agaacactgg ttacgttcaa ggacgtgttt gtggacttta
     7561 cacgtgagga gtggaaattg ctggatactg cgcaacaaat tgtgtatcga aatgtcatgc
     7621 ttgagaatta caagaacctc gtcagtctcg gataccagtt gacgaaaccg gatgtgatcc
     7681 ttaggctcga aaagggggaa gaaccttggc tggtatcggg aggtggttcg ggtggctctg
     7741 gatcaagccc aaagaagaaa cggaaggtgg aagcctcagt gcaggtgaaa agggtgctgg
     7801 aaaaatcccc cggcaaactc ctcgtgaaga tgcccttcca ggcttcccct ggcggaaaag
     7861 gtgaaggggg tggcgcaacc acatctgccc aggtcatggt catcaagcga cctggaagga
     7921 aaagaaaggc cgaggctgac cctcaggcca ttccaaagaa acggggacgc aagccagggt
     7981 ccgtggtcgc agctgcagca gctgaggcta agaaaaaggc agtgaaggaa agctccatcc
     8041 gcagtgtgca ggagactgtc ctgcccatca agaagaggaa gactagggag accgtgtcca
     8101 tcgaggtcaa agaagtggtc aagcccctgc tcgtgtccac cctgggcgaa aaatctggaa
     8161 aggggctcaa aacatgcaag tcacctggac ggaaaagcaa ggagtctagt ccaaaggggc
     8221 gctcaagctc cgcttctagt ccccctaaaa aggaacacca tcaccatcac catcacgccg
     8281 agtctcctaa ggctcctatg ccactgctcc caccacctcc accacctgag ccacagtcaa
     8341 gcgaagaccc catcagccca cccgagcctc aggatctgtc ctctagtatt tgcaaagagg
     8401 aaaagatgcc cagagcaggc agcctggaga gtgatggctg tccaaaagaa cccgccaaga
     8461 cccagcctat ggtggcagcc gctgcaacta ccaccacaac cacaactacc acagtggccg
     8521 aaaaatacaa gcatcgcggc gagggcgaac gaaaggacat tgtgtcaagc tccatgccca
     8581 gacctaaccg ggaggaacca gtcgatagta ggacacccgt gactgagaga gtctcatagg
     8641 aattcaattc taactagacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     8701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     9001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     9061 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     9121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     9181 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     9241 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     9301 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9361 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     9421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9541 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     9601 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9661 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9721 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9781 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     9841 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     9901 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     9961 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    10021 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10081 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10141 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10201 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    10261 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
    10321 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    10381 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10441 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    10501 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    10561 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
    10621 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
    10681 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
    10741 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
    10801 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
    10861 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
    10921 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
    10981 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
    11041 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
    11101 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
    11161 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    11221 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
    11281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    11341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
    11401 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    11461 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    11521 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11581 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11641 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11701 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11761 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11821 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11881 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11941 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    12001 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    12061 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    12121 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    12181 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    12241 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    12301 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    12361 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    12421 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    12481 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    12541 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    12601 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    12661 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    12721 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12781 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    12841 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12901 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12961 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    13021 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    13081 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    13141 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    13201 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    13261 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    13321 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    13381 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    13441 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    13501 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    13561 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    13621 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    13681 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
    13741 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    13801 gggccagata tacgcgtt
//
