LOCUS       40924_1509       10141 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector krtt1c19e-sfGFP-tVE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Eskova A, Garcia-Junco R, Chauvigne F, Maischein H-M., Ammelburg M, 
            Cerda J, Kaderali L, Nusslein-Volhard C, Irion U.
  TITLE     Gain-of-function mutations of mau/DrAqp3a influence zebrafish 
            pigment pattern formation through the tissue environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Eskova A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2016) e-CNV group, Max Planck Institute for 
            Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 10141)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10141)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JAN-2016) e-CNV group, Max Planck Institute for Developmental 
            Biology, Spemannstr. 35-39, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        1119..1137
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1195..3433
                     /label=krtt1c19e
                     /note="krtt1c19e"
                     /regulatory_class="promoter"
     CDS             5186..5902
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     polyA_signal    6841..6975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7038..7058)
                     /label=attB3
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     primer_bind     complement(7066..7082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(7098..7232)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7904..7920)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7957..7975)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7996..8012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8020..8036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8044..8074)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8089..8110)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8398..8986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9160..10017)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10018..10122)
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggta atacgactca ctatagggcg
     1141 aattgggtac cgggcccccc ctcgagcggc cgccagtgtg atggatatct gcagaattcg
     1201 ccctttacac catgggcaat attagccttt ttctggtatg acaattagga ttagaaaaaa
     1261 aggataaata cttaattctc tgttctgaag tggtaatata caaaattttg aacatgaaac
     1321 ttgatgcttt gatatattaa ataacaatgt tataaattat ttccatttac aaacatgtac
     1381 aactacacct aaaattaaaa atacaactaa attaaaaata tatagttctt tctcaagcat
     1441 gctcacactc aaaagttttg accacattca tttatcaagt ctgaaataga acaaattata
     1501 ttatgcttta cgtcttcata atacaaagag caaagcaaat ataacactcc aaaaaatata
     1561 tagttttact catatttttg tcttgtttct aaattaagaa gcatgttcta gacaatagat
     1621 tttttttcta aacagtcaag tatgtttttt ttttgttttg aaacaaatga aataatctgc
     1681 aagtgggata agtaaaacta aaaaaacaaa aactaagaaa atcctttttt ttttgcagtg
     1741 aagttcttat cttttcactt ttaacctgac aaaccgcgtt ttctcttcaa atacgagtga
     1801 cttttcattt aacacctgct ttataatccc tcgttgtcag tcttctgaca tgtgcatagt
     1861 tatttgccct gtggccttag agtaagggtg atgtaaccac tcaaagtcga cagcaagtga
     1921 tttaaaaata ttaataacaa attacaaaaa ggaaaatgac atgttggcat tttgaacccc
     1981 tctgtgcatc aacaaatact gattctagta taaactaaca tgttctactt gaaaatatat
     2041 agttgttaac atctaaaagt agattatatc tatggcctac atcttgccaa caagaaaaat
     2101 ataaattaaa agtgaaatac cactgtctta agtattaata cagcaactag gcattcaaac
     2161 aacataaaac attttgtata tgaattaaag gttttgctac acacatacaa agttagttgt
     2221 acaaatgata aatactgcag ccataattat cgcaggaaaa gttggattca gatatcaatt
     2281 catctacaat tatagcttca ctgacgatgt aaacctgacg tttggctaca ataactggat
     2341 tcaatcttac agacgcattt taactaaact gagtcaagct tacctcgaaa agcgactatc
     2401 aatctagaaa tacattttat aaatggattt taacggcatg aatattaaaa tattctcaaa
     2461 atttgccttc aaacctctgt gcagtcattt gtgatgacct cagcccttct ccactctccc
     2521 tcagaaaacc gcgccatttc tgacggccgc gcgcgcgctc ttgtggtcga gcacgcgatt
     2581 gacgcagcaa aatcaagaag tagaaaagtt aatatttcca tttatttccc tctaaatagg
     2641 acatttctgg ctttcagaga agcaaatttc gacttaggtg actcagttac gagataatcg
     2701 tgtttacaca aacgttttaa cggaactgta gctagaattt ttcatttatc aaaaccgttt
     2761 tccgtgctga ggtataagaa ttcgtcaaat gcagcctatt tctttaatgt tgagtatacg
     2821 accaagcatt cgagagtgat gtgcacaaga gagagtgcat tagactatca agtaacattc
     2881 atcaagtaac atcaagcgct cagaattcag gcaaaacaat atacagtgta cagggaatgt
     2941 ggaacattat taaatctgct aatttaatat aagagggggc taatgcaaat cctacttagc
     3001 acttcagtat aagaactgaa gacacataat aactataaaa tgcaccataa atttcttttt
     3061 atgaaaattc ttgcacttca gcttcactgc ctaagggagg aatttaacgt tgtttaaact
     3121 tggtgagaaa cacacaggta catgcacaaa catgagcttc aaactatctt tatagtaaaa
     3181 ttgctaaaca agtcactgaa gtcaaatcat gagttaccaa cgaggtcagc ttacatcata
     3241 gcgtcttgtt taattatagg cctattcttt tccaattcaa tgggtgttct gtattcatat
     3301 cttcatttgt tcattcccca tcttcagtta ctcaacctta tccaacacag ctcacaagca
     3361 tgaacggctg gtgttcaaac tctgcacatc tttatattca ttttcttttc ggcttagtcc
     3421 atttgttatt caggggtcac cacagcggaa tgaactgctg acttatccag cataagtttt
     3481 atgcagcgga tgcccttcca gctacaaccc attactggga aacacccata cacactcatt
     3541 cacacacata cactatggac aatttagctt tccaaattca cctatactgc atgtctttgg
     3601 atttgtgggg gaaaccggaa cacccggagg taccccacgc caacacagag agaacatgca
     3661 aactcaacac agaaatgcca actgacctag ccgaggcaaa ccagcgacct ggtttgctca
     3721 tttgacctgg tagctcattt ttaaaatgag tgcatcattt gatgagtcag ttaatggcct
     3781 atgatttata agttcttact ggcatttaat tgaagaacct ttaacattgt atttggaaaa
     3841 agtgtattaa aatgttcttt aaaaaattgc tcactgaatg gttcccaggg gaactctttt
     3901 tatggcatct gatggcaatg atgctttaaa agaataattt ttggttcctc aaagaatctt
     3961 tccgtcaaca tttttttgaa gaactatgtt tgtcgttgtg tgaagaacac attttattaa
     4021 ttattaatta caattattaa tgttattttt ataatataaa taaccgtttc caaaaaaatt
     4081 cttcgaatag aaaaagattc aacaaatgtc acaggatctt tgaaccatca agttcattaa
     4141 agaaccttta ttttttttta agaatgcaac agaaacactg taacacccaa cagtcaactt
     4201 tatcaaatga aatgagtgta gtaaactcaa aatttactga aagtttatac aactcattta
     4261 aaaaagaatg ttgaactcag tgtttaaggt ataatgaatg aatttacctc attacattaa
     4321 gttcacagta ctcatatagg ttagtttttt tttactaaaa tggtttgtag cgatcagttt
     4381 cctcaaacgg tttgagttgc cttaacttat tggtttgtac agtactcagt tagtttgagt
     4441 tctcttattt tattgggttt tactgtgttc aaactgcttt gtttactcaa atggattaag
     4501 ttcacagtac tcattagtat tagtttttga acttaaaggg ttttttgcaa tcggtttcct
     4561 caaatggttt gagttacctt aaccttttag gatttacagt gcacaattga actgaaataa
     4621 aggtgttctg acaataatcc cagaacaaca cttttttcag tgaagagttc tttgaaccat
     4681 ttagcgtgaa ggacatttta acaatctaaa gaaccttttc ccccgcaaaa caaatgtttt
     4741 gtgcaaagtt tgcatggatg ttaaaaggtt cttcatagag caatcaatgc caataacggt
     4801 gcaaccttga atatacaaag attttagagc gtgagagtgt ttactacagt aaatccagca
     4861 ctaaaaacaa gaaaatgttt gaacatgtac agagatattc tcatctgaga aacttttgga
     4921 gggggaatgt aagcgtgtat cttacttcct ggaggggatg atcagtcaac tgattttaca
     4981 tctcagcgtc acctttcact ttacgtgatt taagctgata gaattccttg ctgtcagcaa
     5041 gttttgaggt cagcaggtcc ttccttgaca gcttcaccag cgtataaaag aactcaaagc
     5101 ttctgcactg tactcactct tcaggcagag cagagtaaga caccaaccac catccacgga
     5161 tcccatcgat tcgaatagcg ccaccatggt gtctaaagga gaggagctgt tcacaggcgt
     5221 ggtgccaatc ctggtggagc tggatggaga cgtgaacggc cacaagttca gcgtgagagg
     5281 cgagggagag ggagacgcca caaacggcaa gctgacactg aaattcatct gcacaacagg
     5341 caaactgcct gtgccttggc caaccctggt gacaaccctg acatacggag tgcagtgctt
     5401 tagcagatac cctgatcaca tgaaacagca cgatttcttc aagagcgcca tgcctgaggg
     5461 ctacgtgcag gagagaacca tcagcttcaa ggatgacgga acctacaaga caagagccga
     5521 ggtgaagttt gagggagata cactggtgaa cagaatcgag ctgaaaggca tcgatttcaa
     5581 agaggatggc aacatcctgg gacacaaact ggagtacaac ttcaacagcc acaacgtgta
     5641 catcacagcc gataaacaga agaacggcat caaagccaac ttcaagatca gacacaatgt
     5701 ggaggatgga tctgtgcagc tggccgatca ctaccagcag aacacaccta tcggagacgg
     5761 cccagtgctg ctgccagata accactacct gagcacacag agcgtgctgt ctaaagaccc
     5821 taacgagaag agagatcaca tggtgctgct ggagtttgtg acagccgccg gaatcaccct
     5881 gggcatggat gagctgtaca aatccggact cagatctatg ggttggcaga aaagcgttct
     5941 ggataagctt gcgcagactt tccaaatccg caacaagtta ctgcgccagg gattggctga
     6001 atgcttagga actctcatcc tggtgatgtt tggctgtggt tcattggccc agttgaaact
     6061 aagcgaaggt tctcatggtc tctttctcac tgcaaacctt gcttttgggt ttggtgctac
     6121 tcttggaatc ttggtttgcg gccaggtgtc aggcggacat ttaaatcctg ctgttacatt
     6181 tgctctctgc ctcttgggaa gagaaaaatg gagaaagttt cctgtgtact ttctgttcca
     6241 aacactcgga tccttcttgg gtgctgctat tatctttgcc gaataccatg acgcaattta
     6301 tgattatgct ggagaatcaa atgagttgct tgtactgggt gaaaaagaaa cagctgggat
     6361 ttttgctaca tacccaagca aatatctcac ccccctaaat ggattttttg accaggtgat
     6421 aggcacagca tccctgattg tgtgcatcct ggccattgtg gacccctaca acaacccgat
     6481 ccctcaaggt cttgaggcct tcacagtggg attcagcgtc cttatcattg gtctctccat
     6541 gggcttcaat tctggctatg cagtaaaccc agctcaagat tttggacctc gtctttttac
     6601 tgccatggct ggttggggta gtgaagtctt cacagccagg gattattggt ttttggtgcc
     6661 catctttgct ccgttcattg gagccgttat tggtgtgatt gtgtaccagc tgatggtggg
     6721 atggcatgtg gaaggagagg cacgagataa gaaagctaaa gctagagagg aggtgatgaa
     6781 cctcaatgac gtcgccagca aggaatgaga attctgcagt cgacggtacc gcgggcccgg
     6841 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     6901 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6961 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     7021 gtgtgggagg ttttttccaa ctttattata catagttgat aattcactgg ccgtcgtttt
     7081 acggtaccat cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca
     7141 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     7201 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     7261 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     7321 gtatggctga ttatgatcct ctagatcaga tctgcgaaga tacggccacg ggtgctcttg
     7381 atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc
     7441 cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc
     7501 attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca
     7561 atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg
     7621 ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc
     7681 caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt
     7741 tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa
     7801 gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt
     7861 tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga
     7921 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     7981 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8041 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8101 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8161 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8221 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8281 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8341 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8581 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8821 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9061 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9121 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9181 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9241 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9301 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9361 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9421 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9481 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9541 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9601 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9661 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9721 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9781 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9841 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9901 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9961 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10021 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10081 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10141 g
//
