LOCUS 40924_1509 10141 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector krtt1c19e-sfGFP-tVE1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10141) AUTHORS Eskova A, Garcia-Junco R, Chauvigne F, Maischein H-M., Ammelburg M, Cerda J, Kaderali L, Nusslein-Volhard C, Irion U. TITLE Gain-of-function mutations of mau/DrAqp3a influence zebrafish pigment pattern formation through the tissue environment JOURNAL Unpublished REFERENCE 2 (bases 1 to 10141) AUTHORS Eskova A. TITLE Direct Submission JOURNAL Submitted (28-JAN-2016) e-CNV group, Max Planck Institute for Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany REFERENCE 3 (bases 1 to 10141) TITLE Direct Submission REFERENCE 4 (bases 1 to 10141) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (28-JAN-2016) e-CNV group, Max Planck Institute for Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..10141 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 1061..1077 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 1097..1117 /label=attB4 /note="core recombination site for the Gateway(R) BP reaction" promoter 1119..1137 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 1195..3433 /label=krtt1c19e /note="krtt1c19e" /regulatory_class="promoter" CDS 5186..5902 /label=superfolder GFP /note="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" polyA_signal 6841..6975 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind complement(7038..7058) /label=attB3 /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(7066..7082) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" polyA_signal complement(7098..7232) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7904..7920) /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(7957..7975) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7996..8012) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8020..8036 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8044..8074) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(8089..8110) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8398..8986) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9160..10017) /label=AmpR /note="beta-lactamase" promoter complement(10018..10122) /label=AmpR promoter ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa 241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg 301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca 361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa 421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc 481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt 541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat 601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt 661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa 721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta 781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta 841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc 901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc 961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga 1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat 1081 gattacgcca agctatcaac tttgtataga aaagttggta atacgactca ctatagggcg 1141 aattgggtac cgggcccccc ctcgagcggc cgccagtgtg atggatatct gcagaattcg 1201 ccctttacac catgggcaat attagccttt ttctggtatg acaattagga ttagaaaaaa 1261 aggataaata cttaattctc tgttctgaag tggtaatata caaaattttg aacatgaaac 1321 ttgatgcttt gatatattaa ataacaatgt tataaattat ttccatttac aaacatgtac 1381 aactacacct aaaattaaaa atacaactaa attaaaaata tatagttctt tctcaagcat 1441 gctcacactc aaaagttttg accacattca tttatcaagt ctgaaataga acaaattata 1501 ttatgcttta cgtcttcata atacaaagag caaagcaaat ataacactcc aaaaaatata 1561 tagttttact catatttttg tcttgtttct aaattaagaa gcatgttcta gacaatagat 1621 tttttttcta aacagtcaag tatgtttttt ttttgttttg aaacaaatga aataatctgc 1681 aagtgggata agtaaaacta aaaaaacaaa aactaagaaa atcctttttt ttttgcagtg 1741 aagttcttat cttttcactt ttaacctgac aaaccgcgtt ttctcttcaa atacgagtga 1801 cttttcattt aacacctgct ttataatccc tcgttgtcag tcttctgaca tgtgcatagt 1861 tatttgccct gtggccttag agtaagggtg atgtaaccac tcaaagtcga cagcaagtga 1921 tttaaaaata ttaataacaa attacaaaaa ggaaaatgac atgttggcat tttgaacccc 1981 tctgtgcatc aacaaatact gattctagta taaactaaca tgttctactt gaaaatatat 2041 agttgttaac atctaaaagt agattatatc tatggcctac atcttgccaa caagaaaaat 2101 ataaattaaa agtgaaatac cactgtctta agtattaata cagcaactag gcattcaaac 2161 aacataaaac attttgtata tgaattaaag gttttgctac acacatacaa agttagttgt 2221 acaaatgata aatactgcag ccataattat cgcaggaaaa gttggattca gatatcaatt 2281 catctacaat tatagcttca ctgacgatgt aaacctgacg tttggctaca ataactggat 2341 tcaatcttac agacgcattt taactaaact gagtcaagct tacctcgaaa agcgactatc 2401 aatctagaaa tacattttat aaatggattt taacggcatg aatattaaaa tattctcaaa 2461 atttgccttc aaacctctgt gcagtcattt gtgatgacct cagcccttct ccactctccc 2521 tcagaaaacc gcgccatttc tgacggccgc gcgcgcgctc ttgtggtcga gcacgcgatt 2581 gacgcagcaa aatcaagaag tagaaaagtt aatatttcca tttatttccc tctaaatagg 2641 acatttctgg ctttcagaga agcaaatttc gacttaggtg actcagttac gagataatcg 2701 tgtttacaca aacgttttaa cggaactgta gctagaattt ttcatttatc aaaaccgttt 2761 tccgtgctga ggtataagaa ttcgtcaaat gcagcctatt tctttaatgt tgagtatacg 2821 accaagcatt cgagagtgat gtgcacaaga gagagtgcat tagactatca agtaacattc 2881 atcaagtaac atcaagcgct cagaattcag gcaaaacaat atacagtgta cagggaatgt 2941 ggaacattat taaatctgct aatttaatat aagagggggc taatgcaaat cctacttagc 3001 acttcagtat aagaactgaa gacacataat aactataaaa tgcaccataa atttcttttt 3061 atgaaaattc ttgcacttca gcttcactgc ctaagggagg aatttaacgt tgtttaaact 3121 tggtgagaaa cacacaggta catgcacaaa catgagcttc aaactatctt tatagtaaaa 3181 ttgctaaaca agtcactgaa gtcaaatcat gagttaccaa cgaggtcagc ttacatcata 3241 gcgtcttgtt taattatagg cctattcttt tccaattcaa tgggtgttct gtattcatat 3301 cttcatttgt tcattcccca tcttcagtta ctcaacctta tccaacacag ctcacaagca 3361 tgaacggctg gtgttcaaac tctgcacatc tttatattca ttttcttttc ggcttagtcc 3421 atttgttatt caggggtcac cacagcggaa tgaactgctg acttatccag cataagtttt 3481 atgcagcgga tgcccttcca gctacaaccc attactggga aacacccata cacactcatt 3541 cacacacata cactatggac aatttagctt tccaaattca cctatactgc atgtctttgg 3601 atttgtgggg gaaaccggaa cacccggagg taccccacgc caacacagag agaacatgca 3661 aactcaacac agaaatgcca actgacctag ccgaggcaaa ccagcgacct ggtttgctca 3721 tttgacctgg tagctcattt ttaaaatgag tgcatcattt gatgagtcag ttaatggcct 3781 atgatttata agttcttact ggcatttaat tgaagaacct ttaacattgt atttggaaaa 3841 agtgtattaa aatgttcttt aaaaaattgc tcactgaatg gttcccaggg gaactctttt 3901 tatggcatct gatggcaatg atgctttaaa agaataattt ttggttcctc aaagaatctt 3961 tccgtcaaca tttttttgaa gaactatgtt tgtcgttgtg tgaagaacac attttattaa 4021 ttattaatta caattattaa tgttattttt ataatataaa taaccgtttc caaaaaaatt 4081 cttcgaatag aaaaagattc aacaaatgtc acaggatctt tgaaccatca agttcattaa 4141 agaaccttta ttttttttta agaatgcaac agaaacactg taacacccaa cagtcaactt 4201 tatcaaatga aatgagtgta gtaaactcaa aatttactga aagtttatac aactcattta 4261 aaaaagaatg ttgaactcag tgtttaaggt ataatgaatg aatttacctc attacattaa 4321 gttcacagta ctcatatagg ttagtttttt tttactaaaa tggtttgtag cgatcagttt 4381 cctcaaacgg tttgagttgc cttaacttat tggtttgtac agtactcagt tagtttgagt 4441 tctcttattt tattgggttt tactgtgttc aaactgcttt gtttactcaa atggattaag 4501 ttcacagtac tcattagtat tagtttttga acttaaaggg ttttttgcaa tcggtttcct 4561 caaatggttt gagttacctt aaccttttag gatttacagt gcacaattga actgaaataa 4621 aggtgttctg acaataatcc cagaacaaca cttttttcag tgaagagttc tttgaaccat 4681 ttagcgtgaa ggacatttta acaatctaaa gaaccttttc ccccgcaaaa caaatgtttt 4741 gtgcaaagtt tgcatggatg ttaaaaggtt cttcatagag caatcaatgc caataacggt 4801 gcaaccttga atatacaaag attttagagc gtgagagtgt ttactacagt aaatccagca 4861 ctaaaaacaa gaaaatgttt gaacatgtac agagatattc tcatctgaga aacttttgga 4921 gggggaatgt aagcgtgtat cttacttcct ggaggggatg atcagtcaac tgattttaca 4981 tctcagcgtc acctttcact ttacgtgatt taagctgata gaattccttg ctgtcagcaa 5041 gttttgaggt cagcaggtcc ttccttgaca gcttcaccag cgtataaaag aactcaaagc 5101 ttctgcactg tactcactct tcaggcagag cagagtaaga caccaaccac catccacgga 5161 tcccatcgat tcgaatagcg ccaccatggt gtctaaagga gaggagctgt tcacaggcgt 5221 ggtgccaatc ctggtggagc tggatggaga cgtgaacggc cacaagttca gcgtgagagg 5281 cgagggagag ggagacgcca caaacggcaa gctgacactg aaattcatct gcacaacagg 5341 caaactgcct gtgccttggc caaccctggt gacaaccctg acatacggag tgcagtgctt 5401 tagcagatac cctgatcaca tgaaacagca cgatttcttc aagagcgcca tgcctgaggg 5461 ctacgtgcag gagagaacca tcagcttcaa ggatgacgga acctacaaga caagagccga 5521 ggtgaagttt gagggagata cactggtgaa cagaatcgag ctgaaaggca tcgatttcaa 5581 agaggatggc aacatcctgg gacacaaact ggagtacaac ttcaacagcc acaacgtgta 5641 catcacagcc gataaacaga agaacggcat caaagccaac ttcaagatca gacacaatgt 5701 ggaggatgga tctgtgcagc tggccgatca ctaccagcag aacacaccta tcggagacgg 5761 cccagtgctg ctgccagata accactacct gagcacacag agcgtgctgt ctaaagaccc 5821 taacgagaag agagatcaca tggtgctgct ggagtttgtg acagccgccg gaatcaccct 5881 gggcatggat gagctgtaca aatccggact cagatctatg ggttggcaga aaagcgttct 5941 ggataagctt gcgcagactt tccaaatccg caacaagtta ctgcgccagg gattggctga 6001 atgcttagga actctcatcc tggtgatgtt tggctgtggt tcattggccc agttgaaact 6061 aagcgaaggt tctcatggtc tctttctcac tgcaaacctt gcttttgggt ttggtgctac 6121 tcttggaatc ttggtttgcg gccaggtgtc aggcggacat ttaaatcctg ctgttacatt 6181 tgctctctgc ctcttgggaa gagaaaaatg gagaaagttt cctgtgtact ttctgttcca 6241 aacactcgga tccttcttgg gtgctgctat tatctttgcc gaataccatg acgcaattta 6301 tgattatgct ggagaatcaa atgagttgct tgtactgggt gaaaaagaaa cagctgggat 6361 ttttgctaca tacccaagca aatatctcac ccccctaaat ggattttttg accaggtgat 6421 aggcacagca tccctgattg tgtgcatcct ggccattgtg gacccctaca acaacccgat 6481 ccctcaaggt cttgaggcct tcacagtggg attcagcgtc cttatcattg gtctctccat 6541 gggcttcaat tctggctatg cagtaaaccc agctcaagat tttggacctc gtctttttac 6601 tgccatggct ggttggggta gtgaagtctt cacagccagg gattattggt ttttggtgcc 6661 catctttgct ccgttcattg gagccgttat tggtgtgatt gtgtaccagc tgatggtggg 6721 atggcatgtg gaaggagagg cacgagataa gaaagctaaa gctagagagg aggtgatgaa 6781 cctcaatgac gtcgccagca aggaatgaga attctgcagt cgacggtacc gcgggcccgg 6841 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga 6901 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc 6961 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag 7021 gtgtgggagg ttttttccaa ctttattata catagttgat aattcactgg ccgtcgtttt 7081 acggtaccat cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca 7141 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 7201 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 7261 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg 7321 gtatggctga ttatgatcct ctagatcaga tctgcgaaga tacggccacg ggtgctcttg 7381 atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc 7441 cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc 7501 attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca 7561 atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg 7621 ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc 7681 caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt 7741 tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa 7801 gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt 7861 tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga 7921 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg 7981 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 8041 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 8101 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 8161 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 8221 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 8281 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 8341 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 8401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 8461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 8521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 8581 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 8641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 8701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 8761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 8821 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 8881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 8941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 9001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 9061 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 9121 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 9181 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 9241 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 9301 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 9361 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 9421 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 9481 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 9541 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 9601 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 9661 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 9721 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 9781 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 9841 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 9901 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 9961 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 10021 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 10081 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 10141 g //