LOCUS       40924_1469       12820 bp DNA     circular SYN 17-DEC-2018
DEFINITION  BiFC expression vector kanII-pSmRYCE(R), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12820)
  AUTHORS   Offenborn JN, Waadt R, Kudla J.
  TITLE     Visualization and translocation of ternary 
            Calcineurin-A/Calcineurin-B/Calmodulin-2 protein complexes by 
            dual-color trimolecular fluorescence complementation
  JOURNAL   New Phytol. (2015) In press
  PUBMED    25919910
REFERENCE   2  (bases 1 to 12820)
  AUTHORS   Offenborn JN, Waadt R, Kudla J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2015) University of Muenster, Institute for 
            Biology and Plant Biotechnology, Schlossplatz 7, Muenster 48149, 
            Germany
REFERENCE   3  (bases 1 to 12820)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAY-2015) University of Muenster, Institute for Biology and 
            Plant Biotechnology, Schlossplatz 7, Muenster 48149, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..616
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     mobile_element  complement(1317..2084)
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2276..3067
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3369..4514
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6113..6137
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6755..6771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6784..6984)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     CDS             complement(7034..7834)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7884..8063)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        9204..9584
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    9596..9826
                     /label=mCherry 160-236
                     /note="mCherry 160-236"
     CDS             9827..9853
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     misc_feature    9854..9904
                     /label=MCS2
                     /note="MCS2"
     rep_origin      10000..10624
                     /label=oriV
                     /note="incP origin of replication"
     CDS             10832..11479
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     oriT            12163..12272
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             12305..12673
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
       61 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
      121 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
      181 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
      241 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
      301 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
      361 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
      421 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
      481 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
      541 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
      601 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
      661 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg
      721 atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg
      781 ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg
      841 ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg
      901 gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg
      961 ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     1021 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     1081 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     1141 agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     1201 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     1261 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta
     1321 atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc
     1381 agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc
     1441 agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt
     1501 cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag
     1561 ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc
     1621 gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta
     1681 gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc
     1741 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga
     1801 ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa
     1861 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt
     1921 tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca
     1981 ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact ggagcacctc
     2041 aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca
     2101 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     2161 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     2221 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     2281 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     2341 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     2401 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     2461 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     2521 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     2581 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     2641 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     2701 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     2761 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     2821 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     2881 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     2941 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     3001 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     3061 attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact
     3121 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg
     3181 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga
     3241 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag
     3301 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag
     3361 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg
     3421 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg
     3481 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca
     3541 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc
     3601 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta
     3661 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca
     3721 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt
     3781 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg
     3841 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt
     3901 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg
     3961 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga
     4021 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt
     4081 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg
     4141 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg
     4201 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg
     4261 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac
     4321 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc
     4381 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag
     4441 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg
     4501 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg
     4561 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc
     4621 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat
     4681 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat
     4741 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga
     4801 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta
     4861 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc
     4921 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc
     4981 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat
     5041 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt
     5101 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg
     5161 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg
     5221 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac
     5281 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat
     5341 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac
     5401 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc
     5461 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg
     5521 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt
     5581 ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc
     5641 cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt
     5701 tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta
     5761 tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag
     5821 cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca
     5881 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     5941 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     6001 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     6061 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     6121 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     6181 taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     6241 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     6301 aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc
     6361 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6421 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6481 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6541 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6601 agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6661 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6721 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattaatt
     6781 cccatcttga aagaaatata gtttaaatat ttattgataa aataacaagt caggtattat
     6841 agtccaagca aaaacataaa tttattgatg caagtttaaa ttcagaaata tttcaataac
     6901 tgattatatc agctggtaca ttgccgtaga tgaaagactg agtgcgatat tatgtgtaat
     6961 acataaattg atgatatagc tagcttagct catcggggga tcgatccgtc gaagctagct
     7021 tgggtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     7081 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     7141 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     7201 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     7261 catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
     7321 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     7381 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     7441 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     7501 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     7561 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     7621 acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga
     7681 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     7741 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     7801 cgtgcaatcc atcttgttca atccaagctc ccatgggccc tcgactagag tcgagatctg
     7861 gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg
     7921 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc
     7981 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag
     8041 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca
     8101 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcttga tcccctgcgc
     8161 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     8221 ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct
     8281 agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc
     8341 cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg
     8401 gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt
     8461 gaagcttgat ccctgaaagc gacgttggat gttaacatct acaaattgcc ttttcttatc
     8521 gaccatgtac gtaagcgctt acgtttttgg tggacccttg aggaaactgg tagctgttgt
     8581 gggcctgtgg tctcaagatg gatcattaat ttccaccttc acctacgatg gggggcatcg
     8641 caccggtgag taatattgta cggctaagag cgaatttggc ctgtaggatc gatccctgaa
     8701 agcgacgttg gatgttaaca tctacaaatt gccttttctt atcgaccatg tacgtaagcg
     8761 cttacgtttt tggtggaccc ttgaggaaac tggtagctgt tgtgggcctg tggtctcaag
     8821 atggatcatt aatttccacc ttcacctacg atggggggca tcgcaccggt gagtaatatt
     8881 gtacggctaa gagcgaattt ggcctgtaga tctctgaaag cgacgttgga tgttaacatc
     8941 tacaaattgc cttttcttat cgaccatgta cgtaagcgct tacgtttttg gtggaccctt
     9001 gaggaaactg gtagctgttg tgggcctgtg gtctcaagat ggatcattaa tttccacctt
     9061 cacctacgat ggggggcatc gcaccggtga gtaatattgt acggctaaga gcgaatttgg
     9121 cctgtaggat cgatccgcga gctggtcaat cccattgctt ttgaagcagc tcaacattga
     9181 tctctttctc gatcgaggga gatttttcaa atcagtgcgc aagacgtgac gtaagtatcc
     9241 gagtcagttt ttatttttct actaatttgg tcgtttattt cggcgtgtag gacatggcaa
     9301 ccgggcctga atttcgcggg tattctgttt ctattccaac tttttcttga tccgcagcca
     9361 ttaacgactt ttgaatagat acgctgacac gccaagcctc gctagtcaaa agtgtaccaa
     9421 acaacgcttt acagcaagaa cggaatgcgc gtgacgctcg cggtgacgcc atttcgcctt
     9481 ttcagaaatg gataaatagc cttgcttcct attatatctt cccaaattac caatacatta
     9541 cactagcatc tgaatttcat aaccaatctc gatacaccaa atcgactcta gaatgggcgc
     9601 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     9661 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     9721 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     9781 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtacc catacgatgt
     9841 tccagattac gctgggccca ggcctactag tggatccgtc gacctcgagg gtaccgctcc
     9901 cgggtaggag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     9961 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
    10021 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
    10081 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
    10141 aattatcgcg cgcggtgtca tctatgttac tagatcggga attcagatcg gctgagtggc
    10201 tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg
    10261 cgggggtcat aacgtgactc ccttaattct ccgctcatga tcagattgtc gtttcccgcc
    10321 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
    10381 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
    10441 ttgtatgtgc atgccaacca cagggttccc cagatctggc gccggccagc gagacgagca
    10501 agattggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt
    10561 gcaccaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa
    10621 ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg acagcgtcga
    10681 gcgcgacagt gctcagaatt acgatcaggg gtatgttggg tttcacgtct ggcctccgga
    10741 ccagcctccg ctggtccgat tgaacgcgcg gattctttat cactgataag ttggtggaca
    10801 tattatgttt atcagtgata aagtgtcaag catgacaaag ttgcagccga atacagtgat
    10861 ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac ggtctgacga cacgcaaact
    10921 ggcggaacgg ttgggggttc agcagccggc gctttactgg cacttcagga acaagcgggc
    10981 gctgctcgac gcactggccg aagccatgct ggcggagaat catacgcatt cggtgccgag
    11041 agccgacgac gactggcgct catttctgat cgggaatgcc cgcagcttca ggcaggcgct
    11101 gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg caccgcagat
    11161 ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc
    11221 cgtcaatgcg ctgatgacaa tcagctactt cactgttggg gccgtgcttg aggagcaggc
    11281 cggcgacagc gatgccggcg agcgcggcgg caccgttgaa caggctccgc tctcgccgct
    11341 gttgcgggcc gcgatagacg ccttcgacga agccggtccg gacgcagcgt tcgagcaggg
    11401 actcgcggtg attgtcgatg gattggcgaa aaggaggctc gttgtcagga acgttgaagg
    11461 accgagaaag ggtgacgatt gatcaggacc gctgccggag cgcaacccac tcactacagc
    11521 agagccatgt agacaacatc ccctccccct ttccaccgcg tcagacgccc gtagcagccc
    11581 gctacgggct ttttcatgcc ctgccctagc gtccaagcct cacggccgcg ctcggcctct
    11641 ctggcggcct tctggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11701 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11761 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11821 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11881 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11941 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    12001 gcctttctcc cttcgggaag cgtggcgctt ttccgctgca taaccctgct tcggggtcat
    12061 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
    12121 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
    12181 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
    12241 caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa
    12301 gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca aggtgtactg
    12361 ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca tgagcctgtc
    12421 ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg actatgagca
    12481 cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc tgctgaaact
    12541 ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc tcgccctgct
    12601 ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg tggtccgccc
    12661 gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc gtgattgcca
    12721 agcacgtccc catgcgctcc atcaagaaga gcgacttcgc ggagctggtg aagtacatca
    12781 ccgacgagca aggcaagacc gagcgccttt ccgacgctca
//
