LOCUS       40924_1374       13305 bp DNA     circular SYN 17-DEC-2018
DEFINITION  BiFC expression vector hygII-pSmRYNE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Offenborn JN, Waadt R, Kudla J.
  TITLE     Visualization and translocation of ternary 
            Calcineurin-A/Calcineurin-B/Calmodulin-2 protein complexes by 
            dual-color trimolecular fluorescence complementation
  JOURNAL   New Phytol. (2015) In press
  PUBMED    25919910
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Offenborn JN, Waadt R, Kudla J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2015) University of Muenster, Institute for 
            Biology and Plant Biotechnology, Schlossplatz 7, Muenster 48149, 
            Germany
REFERENCE   3  (bases 1 to 13305)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13305)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAY-2015) University of Muenster, Institute for Biology and 
            Plant Biotechnology, Schlossplatz 7, Muenster 48149, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..616
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     mobile_element  complement(1317..2084)
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2276..3067
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3369..4514
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6113..6137
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6755..6771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6784..6984)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     CDS             complement(7026..8048)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8102..8281)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        9422..9802
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    9805..9888
                     /label=MCS
                     /note="MCS"
     CDS             9892..9915
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     rep_origin      10000..10624
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10828..10852
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11317..11964
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     oriT            12648..12757
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             12790..13158
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
       61 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
      121 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
      181 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
      241 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
      301 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
      361 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
      421 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
      481 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
      541 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
      601 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
      661 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg
      721 atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg
      781 ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg
      841 ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg
      901 gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg
      961 ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     1021 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     1081 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     1141 agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     1201 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     1261 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta
     1321 atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc
     1381 agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc
     1441 agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt
     1501 cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag
     1561 ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc
     1621 gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta
     1681 gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc
     1741 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga
     1801 ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa
     1861 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt
     1921 tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca
     1981 ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact ggagcacctc
     2041 aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca
     2101 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     2161 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     2221 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     2281 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     2341 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     2401 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     2461 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     2521 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     2581 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     2641 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     2701 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     2761 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     2821 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     2881 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     2941 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     3001 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     3061 attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact
     3121 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg
     3181 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga
     3241 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag
     3301 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag
     3361 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg
     3421 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg
     3481 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca
     3541 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc
     3601 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta
     3661 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca
     3721 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt
     3781 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg
     3841 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt
     3901 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg
     3961 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga
     4021 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt
     4081 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg
     4141 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg
     4201 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg
     4261 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac
     4321 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc
     4381 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag
     4441 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg
     4501 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg
     4561 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc
     4621 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat
     4681 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat
     4741 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga
     4801 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta
     4861 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc
     4921 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc
     4981 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat
     5041 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt
     5101 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg
     5161 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg
     5221 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac
     5281 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat
     5341 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac
     5401 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc
     5461 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg
     5521 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt
     5581 ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc
     5641 cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt
     5701 tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta
     5761 tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag
     5821 cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca
     5881 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     5941 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     6001 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     6061 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     6121 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     6181 taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     6241 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     6301 aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc
     6361 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6421 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6481 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6541 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6601 agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6661 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6721 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattaatt
     6781 cccatcttga aagaaatata gtttaaatat ttattgataa aataacaagt caggtattat
     6841 agtccaagca aaaacataaa tttattgatg caagtttaaa ttcagaaata tttcaataac
     6901 tgattatatc agctggtaca ttgccgtaga tgaaagactg agtgcgatat tatgtgtaat
     6961 acataaattg atgatatagc tagcttagct catcggggga tcgatcccgg tcggcatcta
     7021 ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     7081 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     7141 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     7201 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     7261 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     7321 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     7381 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     7441 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     7501 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     7561 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     7621 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     7681 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     7741 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     7801 aatgccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     7861 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     7921 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     7981 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     8041 tttttcatat cttattgccc ccctagagtc gagatctgga ttgagagtga atatgagact
     8101 ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat
     8161 ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat
     8221 gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt
     8281 ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac
     8341 tcccttaatt ctccgctcat gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga
     8401 aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca
     8461 attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac
     8521 tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc
     8581 tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc
     8641 tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga agcttgatcc ctgaaagcga
     8701 cgttggatgt taacatctac aaattgcctt ttcttatcga ccatgtacgt aagcgcttac
     8761 gtttttggtg gacccttgag gaaactggta gctgttgtgg gcctgtggtc tcaagatgga
     8821 tcattaattt ccaccttcac ctacgatggg gggcatcgca ccggtgagta atattgtacg
     8881 gctaagagcg aatttggcct gtaggatcga tccctgaaag cgacgttgga tgttaacatc
     8941 tacaaattgc cttttcttat cgaccatgta cgtaagcgct tacgtttttg gtggaccctt
     9001 gaggaaactg gtagctgttg tgggcctgtg gtctcaagat ggatcattaa tttccacctt
     9061 cacctacgat ggggggcatc gcaccggtga gtaatattgt acggctaaga gcgaatttgg
     9121 cctgtagatc tctgaaagcg acgttggatg ttaacatcta caaattgcct tttcttatcg
     9181 accatgtacg taagcgctta cgtttttggt ggacccttga ggaaactggt agctgttgtg
     9241 ggcctgtggt ctcaagatgg atcattaatt tccaccttca cctacgatgg ggggcatcgc
     9301 accggtgagt aatattgtac ggctaagagc gaatttggcc tgtaggatcg atccgcgagc
     9361 tggtcaatcc cattgctttt gaagcagctc aacattgatc tctttctcga tcgagggaga
     9421 tttttcaaat cagtgcgcaa gacgtgacgt aagtatccga gtcagttttt atttttctac
     9481 taatttggtc gtttatttcg gcgtgtagga catggcaacc gggcctgaat ttcgcgggta
     9541 ttctgtttct attccaactt tttcttgatc cgcagccatt aacgactttt gaatagatac
     9601 gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac aacgctttac agcaagaacg
     9661 gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga taaatagcct
     9721 tgcttcctat tatatcttcc caaattacca atacattaca ctagcatctg aatttcataa
     9781 ccaatctcga tacaccaaat cgactctaga gttaaccggg ctcaggcctg gcgcgccact
     9841 agtggatcca tcgatagtac tgtcgacctc gagggtaccg ctcccgggat ggactacaag
     9901 gacgacgatg acaaggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     9961 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
    10021 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
    10081 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
    10141 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
    10201 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
    10261 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
    10321 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
    10381 cccgaggact aggagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
    10441 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
    10501 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
    10561 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
    10621 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattca gatcggctga
    10681 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
    10741 atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga ttgtcgtttc
    10801 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
    10861 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
    10921 tccatttgta tgtgcatgcc aaccacaggg ttccccagat ctggcgccgg ccagcgagac
    10981 gagcaagatt ggccgccgcc cgaaacgatc cgacagcgcg cccagcacag gtgcgcaggc
    11041 aaattgcacc aacgcataca gcgccagcag aatgccatag tgggcggtga cgtcgttcga
    11101 gtgaaccaga tcgcgcagga ggcccggcag caccggcata atcaggccga tgccgacagc
    11161 gtcgagcgcg acagtgctca gaattacgat caggggtatg ttgggtttca cgtctggcct
    11221 ccggaccagc ctccgctggt ccgattgaac gcgcggattc tttatcactg ataagttggt
    11281 ggacatatta tgtttatcag tgataaagtg tcaagcatga caaagttgca gccgaataca
    11341 gtgatccgtg ccgccctgga cctgttgaac gaggtcggcg tagacggtct gacgacacgc
    11401 aaactggcgg aacggttggg ggttcagcag ccggcgcttt actggcactt caggaacaag
    11461 cgggcgctgc tcgacgcact ggccgaagcc atgctggcgg agaatcatac gcattcggtg
    11521 ccgagagccg acgacgactg gcgctcattt ctgatcggga atgcccgcag cttcaggcag
    11581 gcgctgctcg cctaccgcga tggcgcgcgc atccatgccg gcacgcgacc gggcgcaccg
    11641 cagatggaaa cggccgacgc gcagcttcgc ttcctctgcg aggcgggttt ttcggccggg
    11701 gacgccgtca atgcgctgat gacaatcagc tacttcactg ttggggccgt gcttgaggag
    11761 caggccggcg acagcgatgc cggcgagcgc ggcggcaccg ttgaacaggc tccgctctcg
    11821 ccgctgttgc gggccgcgat agacgccttc gacgaagccg gtccggacgc agcgttcgag
    11881 cagggactcg cggtgattgt cgatggattg gcgaaaagga ggctcgttgt caggaacgtt
    11941 gaaggaccga gaaagggtga cgattgatca ggaccgctgc cggagcgcaa cccactcact
    12001 acagcagagc catgtagaca acatcccctc cccctttcca ccgcgtcaga cgcccgtagc
    12061 agcccgctac gggctttttc atgccctgcc ctagcgtcca agcctcacgg ccgcgctcgg
    12121 cctctctggc ggccttctgg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    12181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    12241 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    12301 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    12361 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    12421 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    12481 tgtccgcctt tctcccttcg ggaagcgtgg cgcttttccg ctgcataacc ctgcttcggg
    12541 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
    12601 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
    12661 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
    12721 gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag
    12781 ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc tatcaaggtg
    12841 tactgccttc cagacgaacg aagagcgatt gaggaaaagg cggcggcggc cggcatgagc
    12901 ctgtcggcct acctgctggc cgtcggccag ggctacaaaa tcacgggcgt cgtggactat
    12961 gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg gccgcctggg cggcctgctg
    13021 aaactctggc tcaccgacga cccgcgcacg gcgcggttcg gtgatgccac gatcctcgcc
    13081 ctgctggcga agatcgaaga gaagcaggac gagcttggca aggtcatgat gggcgtggtc
    13141 cgcccgaggg cagagccatg acttttttag ccgctaaaac ggccgggggg tgcgcgtgat
    13201 tgccaagcac gtccccatgc gctccatcaa gaagagcgac ttcgcggagc tggtgaagta
    13261 catcaccgac gagcaaggca agaccgagcg cctttccgac gctca
//
