LOCUS       Exported                4198 bp DNA     circular SYN 05-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ePop
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          152..424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1744..2322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             152..424
                     /gene="E"
                     /label=Lysis protein E
                     /note="Lysis protein E from Enterobacteria phage phiX174. 
                     Accession#: P03639"
     protein_bind    443..459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             661..690
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    867..886
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    900..916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             935..946
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             954..1265
                     /codon_start=1
                     /product="Early stop codon introduced in mutated luxR 
                     results in loss of luxR functionality; the regulation of 
                     this gene is not required for oscillating behavior observed
                     in the study."
                     /label=mutated LuxR
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENKCCK"
     CDS             1744..2322
                     /gene="luxI"
                     /label=Acyl-homoserine-lactone synthase
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio 
                     fischeri. Accession#: P12747"
     terminator      2371..2457
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2622..3210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3298..3392)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3413..4072)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4073..4175)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcagtc ctttgattct aataaattgg atttttgtca cactattgta tcgctgggaa
       61 tacaattact taacataagc acctgtagga tcgtacaggt ttacgcaaga aaatggtttg
      121 ttatagtcga ataaacgcaa gggaggttgg tatggtacgc tggactttgt gggataccct
      181 cgctttcctg ctcctgttga gtttattgct gccgtcattg cttattatgt tcatcccgtc
      241 aacattcaaa cggcctgtct catcatggaa ggcgctgaat ttacggaaaa cattattaat
      301 ggcgtcgagc gtccggttaa agccgctgaa ttgttcgcgt ttaccttgcg tgtacgcgca
      361 ggaaacactg acgttcttac tgacgcagaa gaaaacgtgc gtcaaaaatt acgtgcggaa
      421 ggagtgagac gtctgtgtgg aattgtgagc ggataacaaa ttcacacagg gccctcggac
      481 accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag gaggaacgag
      541 ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa caatgaaaag
      601 gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt ccaagcagag
      661 gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca gttgaaacac
      721 aaacttgaac agctacggaa ctcttgtgcg taaggaaaag taaggaaaac gattccttct
      781 aacagaaatg tcctgagcaa tcacctatga actgtcgact cgagcatagc atttttatcc
      841 ataagattag cggatctaac ctttacaatt gtgagcgctc acaattatga tagattcaat
      901 tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaaggta cccatgaaaa
      961 acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata
     1021 atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac
     1081 tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaattcta gataattacc
     1141 ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag
     1201 attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac aaatgctgta
     1261 aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt
     1321 agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa
     1381 aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt
     1441 ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat
     1501 ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat
     1561 atttcaaaaa tattaggctg cagtgagcgt actgtcactt tccatttaac caatgcgcaa
     1621 atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca
     1681 attgattgcc catactttaa aaattaagta cgggatcccc gaataaacgc aagggaggtt
     1741 ggtatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa
     1801 ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta
     1861 gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt
     1921 gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg
     1981 ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata
     2041 gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt
     2101 gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca
     2161 gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct
     2221 tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg
     2281 tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa attaatgttg ttaagcttcg
     2341 cggccgctta attaattaat ctagaggcat caaataaaac gaaaggctca gtcgaaagac
     2401 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     2461 ccgccctaga cctagggcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2521 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2581 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2641 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2701 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2761 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2821 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2881 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2941 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3001 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3061 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3121 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3181 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3241 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgactag tgcttggatt
     3301 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     3361 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     3421 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     3481 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     3541 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     3601 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     3661 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     3721 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     3781 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     3841 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     3901 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     3961 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     4021 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     4081 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     4141 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc cagatatc
//
