LOCUS       V014904                 4949 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014904
VERSION     V014904
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             152..424
                     /gene="E"
                     /label="Lysis protein E"
                     /note="Lysis protein E from Enterobacteria phage phiX174.
                     Accession#: P03639"
     protein_bind    443..459
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             661..690
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     protein_bind    complement(867..886)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     CDS             953..1669
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1706..2455
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             2495..3073
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     terminator      3122..3208
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3373..3961)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(4049..4143)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4167..4823)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4824..4926)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcagtc ctttgattct aataaattgg atttttgtca cactattgta tcgctgggaa
       61 tacaattact taacataagc acctgtagga tcgtacaggt ttacgcaaga aaatggtttg
      121 ttatagtcga ataaacgcaa gggaggttgg tatggtacgc tggactttgt gggataccct
      181 cgctttcctg ctcctgttga gtttattgct gccgtcattg cttattatgt tcatcccgtc
      241 aacattcaaa cggcctgtct catcatggaa ggcgctgaat ttacggaaaa cattattaat
      301 ggcgtcgagc gtccggttaa agccgctgaa ttgttcgcgt ttaccttgcg tgtacgcgca
      361 ggaaacactg acgttcttac tgacgcagaa gaaaacgtgc gtcaaaaatt acgtgcggaa
      421 ggagtgagac gtctgtgtgg aattgtgagc ggataacaat ttcacacagg gccctcggac
      481 accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag gaggaacgag
      541 ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa caatgaaaag
      601 gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt ccaagcagag
      661 gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca gttgaaacac
      721 aaacttgaac agctacggaa ctcttgtgcg taaggaaaag taaggaaaac gattccttct
      781 aacagaaatg tcctgagcaa tcacctatga actgtcgact cgagcatagc atttttatcc
      841 ataagattag cggatctaac ctttacaatt gtgagcgctc acaattatga tagattcaat
      901 tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaaggta ccatggtgag
      961 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1021 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1081 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1141 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     1201 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1261 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1321 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1381 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1441 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1501 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1561 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1621 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtggagg
     1681 cagtggtgga ggcggtagcg ctagcatgaa aaacataaat gccgacgaca catacagaat
     1741 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat
     1801 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt
     1861 taaatctgat atttcaattc tagataatta ccctaaaaaa tggaggcaat attatgatga
     1921 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat
     1981 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga
     2041 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg
     2101 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt
     2161 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat
     2221 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc
     2281 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggct gcagtgagcg
     2341 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca
     2401 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaagt
     2461 acgggatccc cgaataaacg caagggaggt tggtatgact ataatgataa aaaaatcgga
     2521 ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt atcaagtgtt
     2581 taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag atgagtatga
     2641 taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa gtggatgctg
     2701 gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg aattgcttgg
     2761 tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg ctgtaggtaa
     2821 aaatagctca aagataaata actctgctag tgaaattaca atgaaactat ttgaagctat
     2881 atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat caacagcaat
     2941 agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca aagaaattca
     3001 tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac agtttaaaaa
     3061 agcagtctta aattaatgtt gttaagcttc gcggccgctt aattaattaa tctagaggca
     3121 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     3181 ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctagggcg ttcggctgcg
     3241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3541 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     3601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3781 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3901 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4021 agggattttg gtcatgacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     4081 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     4141 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     4201 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
     4261 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     4321 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     4381 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     4441 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     4501 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     4561 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     4621 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     4681 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     4741 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     4801 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     4861 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     4921 cgatcaacgt ctcattttcg ccagatatc
//
