LOCUS       V013150                15636 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013150
VERSION     V013150
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 15636)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..15636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2687..3865
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3884..3904
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             3914..5629
                     /label="Cas9(N)"
                     /note="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
     CDS             6203..6223
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             8372..8392
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             9587..10300
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    10328..10916
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             11441..11621
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11653..11877
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11923..12351
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        12365..12694
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        12742..12789
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             12808..13179
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    13312..13445
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(13530..13560)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(13575..13596)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(13884..14472)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(14646..15503)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(15504..15608)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg agccccaaga agaagagaaa
     3901 ggtggaggcc agcgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg
     3961 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
     4021 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
     4081 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
     4141 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
     4201 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
     4261 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     4321 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     4381 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     4441 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     4501 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     4561 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     4621 gttcggcaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     4681 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     4741 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     4801 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     4861 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     4921 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     4981 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
     5041 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     5101 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     5161 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     5221 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     5281 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     5341 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
     5401 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     5461 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     5521 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
     5581 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     5641 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     5701 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     5761 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     5821 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     5881 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     5941 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttct ccggactcag
     6001 atctggcagc ggtctcgaga tggaagttag cgctctggaa aaagaagtgt ctgcactcga
     6061 gaaagaagta agtgcccttg agaaggaggt gtccgcactc gagaaggagg tcagcgccct
     6121 ggaaaaggga ggcggaggct ccatgcagct gatccacgac gacagcctga cctttaaaga
     6181 ggacatccag aaagcccagg tggaaaacct gtactttcag tccccatcat caaaggtgtc
     6241 cgccctgaag gaaaaagtaa gcgcactgaa agaaaaggtg agcgcgctga aggagaaagt
     6301 gagcgccctg aaagagaaag tctctgccct taaggaggga ggcggaggct cctccggcca
     6361 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     6421 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     6481 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     6541 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     6601 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     6661 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     6721 cgatgtggac gctatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     6781 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     6841 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     6901 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     6961 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     7021 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     7081 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     7141 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     7201 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     7261 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     7321 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     7381 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     7441 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     7501 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
     7561 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
     7621 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     7681 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     7741 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     7801 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     7861 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     7921 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     7981 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     8041 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     8101 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     8161 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
     8221 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     8281 cgagacacgg atcgacctgt ctcagctggg aggcgacagc gctggaggag gtggaagcgg
     8341 aggaggagga agcggaggag gaggtagcgg acctaagaaa aagaggaagg tggcggccgc
     8401 tggatccgga cgggctgacg cattggacga ttttgatctg gatatgctgg gaagtgacgc
     8461 cctcgatgat tttgaccttg acatgcttgg ttcggatgcc cttgatgact ttgacctcga
     8521 catgctcggc agtgacgccc ttgatgattt cgacctggac atgctgatta acgctagcgg
     8581 cagtggacct acacaggccg gcgagggcac actgtctgaa gctctgctgc agctgcagtt
     8641 cgacgacgag gatctgggag ccctgctggg aaacagcacc gatcctgccg tgttcaccga
     8701 cctggccagc gtggacaaca gcgagttcca gcagctgctg aaccagggca tccctgtggc
     8761 ccctcacacc accgagccca tgctgatgga ataccccgag gccatcaccc ggctcgtgac
     8821 aggcgctcag aggcctcctg atccagctcc tgcccctctg ggagcaccag gcctgcctaa
     8881 tggactgctg tctggcgacg aggacttcag ctctatcgcc gatatggatt tctcagcctt
     8941 gctgggctct ggcagcggca gccgggattc cagggaaggg atgtttttgc cgaagcctga
     9001 ggccggctcc gctattagtg acgtgtttga gggccgcgag gtgtgccagc caaaacgaat
     9061 ccggccattt catcctccag gaagtccatg ggccaaccgc ccactccccg ccagcctcgc
     9121 accaacacca accggtccag tacatgagcc agtcgggtca ctgaccccgg caccagtccc
     9181 tcagccactg gatccagcgc ccgcagtgac tcccgaggcc agtcacctgt tggaggatcc
     9241 cgatgaagag acgagccagg ctgtcaaagc ccttcgggag atggccgata ctgtgattcc
     9301 ccagaaggaa gaggctgcaa tctgtggcca aatggacctt tcccatccgc ccccaagggg
     9361 ccatctggat gagctgacaa ccacacttga gtccatgacc gaggatctga acctggactc
     9421 acccctgacc ccggaattga acgagattct ggataccttc ctgaacgacg agtgcctctt
     9481 gcatgccatg catatcagca caggactgtc catcttcgac acatctctct ttgagggcag
     9541 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagtga gcaagggcga
     9601 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     9661 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     9721 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     9781 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     9841 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     9901 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     9961 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
    10021 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
    10081 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
    10141 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
    10201 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
    10261 cgccgccggg atcactctcg gcatggacga gctgtacaag taagaattcg atatcaagct
    10321 tatcggtaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    10381 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    10441 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    10501 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    10561 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    10621 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    10681 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
    10741 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    10801 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
    10861 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
    10921 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
    10981 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
    11041 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
    11101 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
    11161 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
    11221 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
    11281 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
    11341 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
    11401 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
    11461 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    11521 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    11581 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
    11641 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
    11701 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
    11761 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
    11821 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
    11881 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
    11941 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    12001 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    12061 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    12121 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    12181 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    12241 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    12301 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
    12361 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    12421 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    12481 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    12541 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    12601 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    12661 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    12721 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
    12781 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
    12841 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
    12901 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
    12961 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
    13021 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
    13081 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
    13141 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
    13201 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    13261 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    13321 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    13381 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    13441 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    13501 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    13561 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    13621 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    13681 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    13741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    13801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    13861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    13921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    13981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    14041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    14101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    14161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    14221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    14281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    14341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    14401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    14461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    14521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    14581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    14641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    14701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    14761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    14821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    14881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    14941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    15001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    15061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    15121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    15181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    15241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    15301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    15361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    15421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    15481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    15541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    15601 gttccgcgca catttccccg aaaagtgcca cctgac
//
