LOCUS       V000071                13870 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000071
VERSION     V000071
KEYWORDS    aMHC-eGFP-Rex-Neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot
            JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R,
            Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen
            HS, Mercola M
  TITLE     Lentiviral vectors and protocols for creation of stable hESC lines
            for fluorescent tracking and drug resistance selection of
            cardiomyocytes.
  JOURNAL   PLoS ONE. 2009  . 4(4):e5046.
   PUBMED   19352491
REFERENCE   2  (bases 1 to 13870)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13870)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE.
            2009  . 4(4):e5046."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label="AmpR promoter"
     CDS             132..989
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1163..1751
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     1905..1922
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2075..2105
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label="M13/pUC Reverse"
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     2137..2153
                     /label="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2174..2192
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2220..2447
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2448..2628
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    3290..3523
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             3708..3752
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             3901..3942
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    4019..4135
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        4192..9650
                     /label="alpha-MHC(long)"
                     /note="Mouse alpha-cardiac myosin heavy chain promoter (5.4
                     kb)"
     CDS             9670..10386
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    10405..10993
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             11735..12535
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             12611..12844
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12916..13050
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      13056..13191
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(13224..13242)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13252..13269)
                     /label="M13 Forward"
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     complement(13252..13268)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(13261..13283)
                     /label="M13/pUC Forward"
                     /note="In lacZ gene"
     rep_origin      13413..13868
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaacaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     4141 tcacgagact agcctcgagc tccaccgcgg tggcggccgc tctagaacta gtggatcctg
     4201 caaggtcaca caagggtctc cacccaccag gtgccctagt ctcaatttca gtttccatgc
     4261 cttgttctca caatgctggc ctccccagag ctaatttgga ctttgttttt atttcaaaag
     4321 ggcctgaatg aggagtagat cttgtgctac ccagctctaa gggtgcccgt gaagccctca
     4381 gacctggagc ctttgcaaca gccctttagg tggaagcaga ataaagcaat tttccttaaa
     4441 gccaaaatcc tgcctctaga ctcttcttct ctgacctcgg tccctgggct ctagggtggg
     4501 gaggtggggc ttggaagaag aaggtgggga agtggcaaaa gccgatccct agggccctgt
     4561 gaagttcgga gccttccctg tacagcactg gctcatagat cctcctccag ccaaacatag
     4621 caagaagtga tacctccttt gtgacttccc caggcccagt acctgtcagg ttgaaacagg
     4681 atttagagaa gcctctgaac tcacctgaac tctgaagctc atccaccaag caagcaccta
     4741 ggtgccactg ctagttagta tcctacgctg ataatatgca gagctgggcc acagaagtcc
     4801 tggggtgtag gaactgacca gtgacttttc agtcggcaaa ggtatgaccc cctcagcaga
     4861 tgtagtaatg tccccttaga tcccatccca ggcaggtctc taagaggaca tgggatgaga
     4921 gatgtagtca tgtggcattc caaacacagc tatccacagt gtcccttgcc ccttccactt
     4981 agccaggagg acagtaacct tagcctatct ttcttcctcc ccatcctccc aggacacacc
     5041 ccctggtctg cagtattcat ttcttccttc acgtcccctc tgtgacttcc atttgcaagg
     5101 cttttgacct ctgcagctgc tggaagatag agtttggccc taggtgtggc aagccatctc
     5161 aagagaaagc agacaacagg gggaccagat tttggaagga tcaggaacta aatcactggc
     5221 gggcctgggg gtagaaaaaa gagtgagtga gtccgctcca gctaagccaa gctagtcccc
     5281 gagatactct gccacagctg ggctgctcgg ggtagcttta ggaatgtggg tctgaaagac
     5341 aatgggattg gaagacatct ctttgagtct cccctcaacc ccacctacag acacactcgt
     5401 gtgtggccag actcctgttc aacagccctc tgtgttctga ccactgagct aggcaaccag
     5461 agcatgggcc ctgtgctgag gatgaagagt tggttaccaa tagcaaaaac agcaggggag
     5521 ggagaacaga gaacgaaata aggaaggaag aaggaaaggc cagtcaatca gatgcagtca
     5581 gaagagatgg gaagccaaca cacagcttga gcagaggaaa cagaaaaggg agagattctg
     5641 ggcataagga ggccacagaa agaagagccc aggcccccca agtctcctct ttataccctc
     5701 atcccgtctc ccaattaagc ccactcttct tcctagatca gacctgagct gcagcgaaga
     5761 gacccgtagg gaggatcaca ctggatgaag gagatgtgtg gagaagtcca gggcaaccta
     5821 agagccagag cctaaaagag caagagataa aggtgcttca aaggtggcca ggctgtgcac
     5881 acagagggtc gaggactggt ggtagagcct caagataagg atgatgctca gaatgggcgg
     5941 ggggggggat tctggggggg ggagagagaa ggtgagaagg agcctggaac agagaatctg
     6001 gaagcgctgg aaacgatacc ataaagggaa gaacccaggc tacctttaga tgtaaatcat
     6061 gaaagacagg gagaagggaa gctggagaga gtagaaggac cccggggcaa gacatggaag
     6121 caaggacaag ccaggttgag cgctccgtga aatcagcctg ctgaaggcag agccctggta
     6181 tgagcaccag aacagcagag gctagggtta atgtcgagac agggaacaga aggtagacac
     6241 aggaacagac agagacgggg gagccaggta acaaaggaat ggtccttctc acctgtggcc
     6301 agagcgtcca tctgtgtcca catactctag aatgttcatc agactgcagg gctggcttgg
     6361 gaggcagctg gaaagagtat gtgagagcca ggggagacaa gggggcctag gaaaggaaga
     6421 agagggcaaa ccaggccaca caagagggca gagcccagaa ctgagttaac tccttccttg
     6481 ttgcatcttc cataggaggc agtgggaact ctgtgaccac catcccccat gagcccccac
     6541 tacccatacc aagtttggcc tgagtggcat tctaggttcc ctgaggacag agcctggcct
     6601 ttgtctcttg gacctgaccc aagctgaccc aatgttctca gtaccttatc atgccctcaa
     6661 gagcttgaga accaggcagt gacatattag gccatgggct aaccctggag cttgcacaca
     6721 ggagcctcaa gtgacctcca gggacacagc tgcagacagg tggcctttat ccccaaagag
     6781 caaccatttg gcataggtgg ctgcaaatgg gaatgcaagg ttgaatcagg tcccttcaag
     6841 aatactgcat gcaagaccta agacccctgg agagaggggt atgctcctgc ccccacccac
     6901 cataagggga gtgaactatc ctagggggct ggcgaccttg gggagacacc acattactga
     6961 gagtgctgag cccagaaaaa ctgaccgccc tgtgtcctgc ccacctccac actctagagc
     7021 tatattgaga ggtgacagta gatagggtgg gagctggtag cagggagagt gttcctgggt
     7081 gtgagggtgt aggggaaagc cagagcaggg gagtctggct ttgtctcctg aacacaatgt
     7141 ctacttagtt ataacaggca tgacctgcta aagacccaac atctacgacc tctgaaaaga
     7201 cagcagccct ggaggacagg ggttgtctct gagccttggg tgcttgatgg tgccacaaag
     7261 gagggcatga gtgtgagtat aaggccccag gagcgttaga gaagggcact tgggaagggg
     7321 tcagtctgca gagcccctat ccatggaatc tggagcctgg ggccaactgg tgtaaatctc
     7381 tgggcctgcc aggcattcaa agcagcacct gcatcctctg gcagcctggg gaggcggaag
     7441 ggagcaaccc cccacttata ccctttctcc ctcagcccca ggattaacac ctctggcctt
     7501 cccccttccc acctcccatc aggagtggag ggttgcagag ggagggtaaa aacctacatg
     7561 tccaaacatc atggtgcacg atatatggat cagtatgtgt agaggcaaga aaggaaatct
     7621 gcaggcttaa ctgggttaat gtgtaaagtc tgtgtgcatg tgtgtgtgtc tgactgaaaa
     7681 cgggcatggc tgtgcagctg ttcagttctg tgcgtgaggt taccagactg caggtttgtg
     7741 tgtaaattgc ccaaggcaaa gtgggtgaat cccttccatg gtttaaagag attggatgat
     7801 ggcctgcatc tcaaggacca tggaaaatag aatggacact ctatatgtgt ctctaagcta
     7861 aggtagcaag gtctttggag gacacctgtc tagagatgtg ggcaacagag actacagaca
     7921 gtatctgtac agagtaagga gagagaggag ggggtgtaga attctcttac tatcaaaggg
     7981 aaactgagtc gtgcacctgc aaagtggatg ctctccctag acatcatgac tttgtctctg
     8041 gggagccagc actgtggaac ttcaggtctg agagagtagg aggctcccct cagcctgaag
     8101 ctatgcagat agccagggtt gaaaggggga agggagagcc tgggatggga gcttgtgtgt
     8161 tggaggcagg ggacagatat taagcctgga agagaaggtg acccttaccc agttgttcaa
     8221 ctcacccttc agattaaaaa taactgaggt aagggcctgg gtaggggagg tggtgtgaga
     8281 cgctcctgtc tctcctctat ctgcccatcg gccctttggg gaggaggaat gtgcccaagg
     8341 actaaaaaaa ggccatggag ccagaggggc gagggcaaca gacctttcat gggcaaacct
     8401 tggggccctg ctgtcctcct gtcacctcca gagccaaggg atcaaaggag gaggagccag
     8461 gacaggaggg aagtgggagg gagggtccca gcagaggact ccaaatttag gcagcaggca
     8521 tatgggatgg gatataaagg ggctggagca ctgagagctg tcagagattt ctccaaccca
     8581 ggtaagaggg agtttcgggt gggggctctt cacccacacc agacctctcc ccacctagaa
     8641 ggaaactgcc tttcctggaa gtggggttca ggccggtcag agatctgaca gggtggcctt
     8701 ccaccagcct gggaagttct cagtggcagg aggtttccac aagaaacact ggatgcccct
     8761 tcccttacgc tgtcttctcc atcttcctcc tggggatgct cctccccgtc ttggtttatc
     8821 ttggctcttc gtcttcagca agatttgccc tgtgctgtcc actccatctt tctctactgt
     8881 ctccgtgcct tgccttgcct tcttgcgtgt ccttcctttc cacccatttc tcacttcacc
     8941 ttttctcccc ttctcatttg tattcatcct tccttccttc cttccttcct tccttccttc
     9001 cttccttcct tcctttctcc cttccttcct tccttccttc cttccttcct tccttccttc
     9061 ctgtgtcaga gtgctgagaa tcacacctgg ggttcccacc cttatgtaaa caatcttcca
     9121 gtgagccaca gcttcagtgc tgctgggtgc tctcttacct tcctcacccc ctggcttgtc
     9181 ctgttccatc ctggtcagga tctctagatt ggtctcccag cctctgctac tcctcttcct
     9241 gcctgttcct ctctctgtcc agctgcgcca ctgtggtgcc tcgttccagc tgtggtccac
     9301 attcttcagg attctctgaa aagttaacca ggtgagaatg tttcccctgt agacagcaga
     9361 tcacgattct cccggaagtc aggcttccag ccctctcttt ctctgcccag ctgcccggca
     9421 ctcttagcaa acctcaggca cccttacccc acatagacct ctgacagaga agcaggcact
     9481 ttacatggag tcctggtggg agagccatag gctacggtgt aaaagaggca gggaagtggt
     9541 ggtgtaggaa agtcaggact tcacatagaa gcctagccca caccagaaat gacagacaga
     9601 tccctcctat ctcccccata agagtttgag tcgacggtac cgcgggcccg ggatccaccg
     9661 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     9721 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     9781 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     9841 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     9901 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     9961 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
    10021 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
    10081 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
    10141 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
    10201 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
    10261 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
    10321 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
    10381 tacaagtaaa gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
    10441 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    10501 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    10561 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    10621 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    10681 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    10741 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
    10801 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    10861 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    10921 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    10981 gccgcctccc cgcctgaatt tcaccggcga ccgattcctc ccgataagta aattacagaa
    11041 aagcttcagg ttaaatgtaa gaggatctta aatattgatt tggtttttgt cttaatggtg
    11101 cccggcgtat ttataagaac cattaaattt cactagaccg tttgtagtca ggggtgaacg
    11161 ctgcatgacc cacgctctca aagtgagttg tgttgctttg cgggaatcca gcagtcacat
    11221 taatgctgga aaaagttcag gcaactagtg tactttgtag cggggtccgg gagaggctgg
    11281 ggtctagagt ggcgatggga cgaaagggta aagaaagttt tcggtgtgtg tgtgtgtgtg
    11341 tgtgtgtgtg tgtgtgtgtg tgtgtgtgta ggtaggtagg taggtagaaa ctgcatcctc
    11401 tgcttgtgta aatccggtta catgtgtgta acagaggtac tgagatgtga ctgagtctca
    11461 aggccaggcg atcgggattc agaagaggca tttgcataac tgagcaagag cctttgcccc
    11521 acccttccac gcggacccaa gacaagcgga cagctctggg tgggtcacct tgaagccagg
    11581 ggcccgccca catccccgcc cacacccacc ttgagcgctt ctcattgggc attccagcct
    11641 acccagctcg gagttagtta ctccgtaagt gtggccggaa cagagttcgt ccatctaaaa
    11701 agggagggga ccggcgaaca catctagagc caccatggga tcggccattg aacaagatgg
    11761 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
    11821 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
    11881 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
    11941 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
    12001 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
    12061 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
    12121 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
    12181 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
    12241 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt
    12301 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
    12361 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
    12421 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
    12481 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaag
    12541 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
    12601 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
    12661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    12721 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    12781 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    12841 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
    12901 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    12961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    13021 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
    13081 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    13141 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    13201 gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
    13261 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    13321 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    13381 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc
    13441 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    13501 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    13561 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    13621 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    13681 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    13741 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    13801 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
    13861 tacaatttcc
//
