LOCUS       40924_49777        7775 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UAS-less reporter vector YIpMELbeta, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Zero background yeast reporter plasmids
  JOURNAL   Gene 247 (1-2), 53-61 (2000)
  PUBMED    10773444
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2003) Institute for Microbiology, Frankfurt 
            University, Marie Curie Strasse 9, Frankfurt 60439, Germany
REFERENCE   3  (bases 1 to 7775)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 247 
            (1-2), 53-61 (2000)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-SEP-2003) Institute for Microbiology, Frankfurt University, 
            Marie Curie Strasse 9, Frankfurt 60439, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             365..3373
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VSRLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPA
                     PEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYS
                     LTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENR
                     LAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEA
                     EVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLW
                     SAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHH
                     PLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG
                     MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSV
                     DPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCE
                     YAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTP
                     NDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWM
                     VALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHI
                     SAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDK
                     KQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD
                     AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQV
                     AERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQ
                     WRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAQFQL
                     SAGRYHYQLVWCQK"
     CDS             complement(3574..4374)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4375..4585)
                     /label=URA3 promoter
     promoter        5389..5493
                     /label=AmpR promoter
     CDS             5494..6351
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6525..7113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgagaagaa gaaaggaaga catgtattac attatccaac aaaaaatggt tcttgacgtc
       61 tacaaatcaa gaatcttaaa gacattgaac gaagtagctg aataaaaatt atgaaaacta
      121 taaaaactat aaaaactgta cttaagtcct caataaaaca taaacttctt actgtataag
      181 gttttcgata atttcttact tgattctagg agagcaacgg taataaaagc aaacgacgat
      241 gaacggatcc ggagcttggc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
      301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      361 acaggtttcc cgacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      421 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      481 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
      541 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
      601 ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
      661 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
      721 gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt
      781 cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc
      841 cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt atctggaaga
      901 tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac
      961 tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt
     1021 actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc
     1081 tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat
     1141 cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa
     1201 actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga
     1261 cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa
     1321 tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca
     1381 tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat
     1441 gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta
     1501 cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg
     1561 catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg
     1621 cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg
     1681 gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga
     1741 tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat
     1801 ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc
     1861 catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc
     1921 ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta
     1981 tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga
     2041 tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg
     2101 ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga
     2161 agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac
     2221 cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga
     2281 tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt
     2341 gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg
     2401 cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca
     2461 gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca
     2521 tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa
     2581 ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac tgctgacgcc
     2641 gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac
     2701 ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga
     2761 agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc
     2821 tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga
     2881 tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc
     2941 ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg
     3001 attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga
     3061 tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg
     3121 cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaacat
     3181 cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc tgcacgcgga
     3241 agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg
     3301 gagcccgtca gtatcggcgc aattccagct gagcgccggt cgctaccatt accagttggt
     3361 ctggtgtcaa aaataataat aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa
     3421 atccattatg tactatttcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3481 tcacaccgca tagggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag
     3541 tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata
     3601 tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc
     3661 aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt
     3721 tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat
     3781 caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac
     3841 aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag
     3901 ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc
     3961 ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt
     4021 aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt
     4081 accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc
     4141 ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt
     4201 tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg
     4261 aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc
     4321 agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta
     4381 tcttcgtttc ggtttttgtt ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt
     4441 cttcaacact acatatgcgt atatatacca atctaagtct gtgctccttc cttcgttctt
     4501 ccttctgttc ggagattacc gaatcaaaaa aatttcaaag aaaccgaaat caaaaaaaag
     4561 aataaaaaaa aaatgatgaa ttgaaaagct cttgttaccc atcattgaat tttgaacatc
     4621 cgaacctggg agttttccct gaaacagata gtatatttga acctgtataa taatatatag
     4681 tctagcgctt tacggaagac aatgtatgta tttcggttcc tggagaaact attgcatcta
     4741 ttgcataggt aatcttgcac gtcgcatccc cggttcattt tctgcgtttc catcttgcac
     4801 ttcaatagca tatctttgtt attttagtag ctcgttacag tccggtgcgt ttttggtttt
     4861 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt
     4921 tctagctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc
     4981 ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc
     5041 tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta
     5101 aatgcgttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5161 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5221 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5281 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5341 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5401 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5461 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5521 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5581 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5641 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5701 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5761 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5821 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5881 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5941 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6001 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6061 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6121 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6181 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6241 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6301 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6361 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6421 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6481 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6541 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6601 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6661 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6721 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6781 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6841 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6901 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6961 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7021 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7081 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7141 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7201 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7261 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7321 cccgcgcgtt ggccgattca ttcccgaatt gggaagcttt cttttttact ttaatattat
     7381 gtatactgaa aaatgcacgt gatgataaga ctttggaaat ttgtgtaaaa cccccatttt
     7441 tttttgctgc tgctattgcc aaagncaaca agtcttcagg agacatcaac actaagtttc
     7501 taccccgtct tcccctagaa ttctttctgt acgctcaggg tgggccttta aaggatagca
     7561 ccctaccgaa gtcgacttct aagtaaacac cattactagg agatgactaa atctggaaaa
     7621 cacatggtgg tctgaatgcg tctagtctct gccataaaca taacatgttt gttttaatgc
     7681 attctcgtgt ttaatcgaca ttaatgtggg gggagaaaga catcccatcc ctgaaaggtt
     7741 tttccaggga atagtcagga cgcattggct ttcac
//
