LOCUS       40924_49677       10928 bp DNA     circular SYN 18-DEC-2018
DEFINITION  VEEV replicon vector YFV-C2opt-NS2mut, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Trans-packaging of Yellow Fever virus replicons: an approach for 
            flaviviral antigen production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2005) Department of Microbiology and Immunology, 
            University of Texas Medical Branch, 301 University Boulevard, 
            Galveston, TX 77550-1019, USA
REFERENCE   3  (bases 1 to 10928)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10928)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2005) Department of Microbiology and Immunology, University 
            of Texas Medical Branch, 301 University Boulevard, Galveston, TX 
            77550-1019, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..45
                     /note="5'UTR of Venezuelan equine encephalitis virus VEE
                     TC83"
     misc_feature    46..1650
                     /note="nsP1 of Venezuelan equine encephalitis virus VEE
                     TC83"
     RBS             1536..1544
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    1651..4032
                     /note="nsP2 of Venezuelan equine encephalitis virus VEE
                     TC83"
     misc_difference 3866
                     /replace="a"
                     /note="A->T mutation from TC83 sequence produce Leu mutant
                     in nsP2"
     misc_feature    4033..5703
                     /note="nsP3 of Venezuelan equine encephalitis virus VEE
                     TC83"
     misc_feature    5704..7524
                     /note="nsP4 of Venezuelan equine encephalitis virus VEE
                     TC83"
     misc_feature    7502..7546
                     /note="subgenomic promoter of Venezuelan equine
                     encephalitis virus VEE TC83"
     misc_feature    7571..7933
                     /note="Yellow fever virus 17D capsid protein (synthetic, 
                     codon-optimized)"
     misc_feature    7934..7993
                     /note="first 20 aa from PR of Yellow fever virus 17D
                     (synthetic, codon-optimized)"
     misc_feature    7997..8041
                     /note="subgenomic promoter of Venezuelan equine
                     encephalitis virus VEE TC83 (second copy)"
     CDS             8071..8667
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    8747..9102
                     /note="3'UTR of Venezuelan equine encephalitis virus VEE
                     TC83"
     promoter        9166..9270
                     /label=AmpR promoter
     CDS             9271..10128
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      10302..10890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatgggcggc gcatgagaga agcccagacc aattacctac ccaaaatgga gaaagttcac
       61 gttgacatcg aggaagacag cccattcctc agagctttgc agcggagctt cccgcagttt
      121 gaggtagaag ccaagcaggt cactgataat gaccatgcta atgccagagc gttttcgcat
      181 ctggcttcaa aactgatcga aacggaggtg gacccatccg acacgatcct tgacattgga
      241 agtgcgcccg cccgcagaat gtattctaag cacaagtatc attgtatctg tccgatgaga
      301 tgtgcggaag atccggacag attgtataag tatgcaacta agctgaagaa aaactgtaag
      361 gaaataactg ataaggaatt ggacaagaaa atgaaggagc tggccgccgt catgagcgac
      421 cctgacctgg aaactgagac tatgtgcctc cacgacgacg agtcgtgtcg ctacgaaggg
      481 caagtcgctg tttaccagga tgtatacgcg gttgacggac cgacaagtct ctatcaccaa
      541 gccaataagg gagttagagt cgcctactgg ataggctttg acaccacccc ttttatgttt
      601 aagaacttgg ctggagcata tccatcatac tctaccaact gggccgacga aaccgtgtta
      661 acggctcgta acataggcct atgcagctct gacgttatgg agcggtcacg tagagggatg
      721 tccattctta gaaagaagta tttgaaacca tccaacaatg ttctattctc tgttggctcg
      781 accatctacc acgagaagag ggacttactg aggagctggc acctgccgtc tgtatttcac
      841 ttacgtggca agcaaaatta cacatgtcgg tgtgagacta tagttagttg cgacgggtac
      901 gtcgttaaaa gaatagctat cagtccaggc ctgtatggga agccttcagg ctatgctgct
      961 acgatgcacc gcgagggatt cttgtgctgc aaagtgacag acacattgaa cggggagagg
     1021 gtctcttttc ccgtgtgcac gtatgtgcca gctacattgt gtgaccaaat gactggcata
     1081 ctggcaacag atgtcagtgc ggacgacgcg caaaaactgc tggttgggct caaccagcgt
     1141 atagtcgtca acggtcgcac ccagagaaac accaatacca tgaaaaatta ccttttgccc
     1201 gtagtggccc aggcatttgc taggtgggca aaggaatata aggaagatca agaagatgaa
     1261 aggccactag gactacgaga tagacagtta gtcatggggt gttgttgggc ttttagaagg
     1321 cacaagataa catctattta taagcgcccg gatacccaaa ccatcatcaa agtgaacagc
     1381 gatttccact cattcgtgct gcccaggata ggcagtaaca cattggagat cgggctgaga
     1441 acaagaatca ggaaaatgtt agaggagcac aaggagccgt cacctctcat taccgccgag
     1501 gacgtacaag aagctaagtg cgcagccgat gaggctaagg aggtgcgtga agccgaggag
     1561 ttgcgcgcag ctctaccacc tttggcagct gatgttgagg agcccactct ggaagccgat
     1621 gtcgacttga tgttacaaga ggctggggcc ggctcagtgg agacacctcg tggcttgata
     1681 aaggttacca gctacgatgg cgaggacaag atcggctctt acgctgtgct ttctccgcag
     1741 gctgtactca agagtgaaaa attatcttgc atccaccctc tcgctgaaca agtcatagtg
     1801 ataacacact ctggccgaaa agggcgttat gccgtggaac cataccatgg taaagtagtg
     1861 gtgccagagg gacatgcaat acccgtccag gactttcaag ctctgagtga aagtgccacc
     1921 attgtgtaca acgaacgtga gttcgtaaac aggtacctgc accatattgc cacacatgga
     1981 ggagcgctga acactgatga agaatattac aaaactgtca agcccagcga gcacgacggc
     2041 gaatacctgt acgacatcga caggaaacag tgcgtcaaga aagaactagt cactgggcta
     2101 gggctcacag gcgagctggt ggatcctccc ttccatgaat tcgcctacga gagtctgaga
     2161 acacgaccag ccgctcctta ccaagtacca accatagggg tgtatggcgt gccaggatca
     2221 ggcaagtctg gcatcattaa aagcgcagtc accaaaaaag atctagtggt gagcgccaag
     2281 aaagaaaact gtgcagaaat tataagggac gtcaagaaaa tgaaagggct ggacgtcaat
     2341 gccagaactg tggactcagt gctcttgaat ggatgcaaac accccgtaga gaccctgtat
     2401 attgacgaag cttttgcttg tcatgcaggt actctcagag cgctcatagc cattataaga
     2461 cctaaaaagg cagtgctctg cggggatccc aaacagtgcg gtttttttaa catgatgtgc
     2521 ctgaaagtgc attttaacca cgagatttgc acacaagtct tccacaaaag catctctcgc
     2581 cgttgcacta aatctgtgac ttcggtcgtc tcaaccttgt tttacgacaa aaaaatgaga
     2641 acgacgaatc cgaaagagac taagattgtg attgacacta ccggcagtac caaacctaag
     2701 caggacgatc tcattctcac ttgtttcaga gggtgggtga agcagttgca aatagattac
     2761 aaaggcaacg aaataatgac ggcagctgcc tctcaagggc tgacccgtaa aggtgtgtat
     2821 gccgttcggt acaaggtgaa tgaaaatcct ctgtacgcac ccacctcaga acatgtgaac
     2881 gtcctactga cccgcacgga ggaccgcatc gtgtggaaaa cactagccgg cgacccatgg
     2941 ataaaaacac tgactgccaa gtaccctggg aatttcactg ccacgataga ggagtggcaa
     3001 gcagagcatg atgccatcat gaggcacatc ttggagagac cggaccctac cgacgtcttc
     3061 cagaataagg caaacgtgtg ttgggccaag gctttagtgc cggtgctgaa gaccgctggc
     3121 atagacatga ccactgaaca atggaacact gtggattatt ttgaaacgga caaagctcac
     3181 tcagcagaga tagtattgaa ccaactatgc gtgaggttct ttggactcga tctggactcc
     3241 ggtctatttt ctgcacccac tgttccgtta tccattagga ataatcactg ggataactcc
     3301 ccgtcgccta acatgtacgg gctgaataaa gaagtggtcc gtcagctctc tcgcaggtac
     3361 ccacaactgc ctcgggcagt tgccactgga agagtctatg acatgaacac tggtacactg
     3421 cgcaattatg atccgcgcat aaacctagta cctgtaaaca gaagactgcc tcatgcttta
     3481 gtcctccacc ataatgaaca cccacagagt gacttttctt cattcgtcag caaattgaag
     3541 ggcagaactg tcctggtggt cggggaaaag ttgtccgtcc caggcaaaat ggttgactgg
     3601 ttgtcagacc ggcctgaggc taccttcaga gctcggctgg atttaggcat cccaggtgat
     3661 gtgcccaaat atgacataat atttgttaat gtgaggaccc catataaata ccatcactat
     3721 cagcagtgtg aagaccatgc cattaagctt agcatgttga ccaagaaagc ttgtctgcat
     3781 ctgaatcccg gcggaacctg tgtcagcata ggttatggtt acgctgacag ggccagcgaa
     3841 agcatcattg gtgctatagc gcggctgttc aagttttccc gggtatgcaa accgaaatcc
     3901 tcacttgaag agacggaagt tctgtttgta ttcattgggt acgatcgcaa ggcccgtacg
     3961 cacaatcctt acaagctttc atcaaccttg accaacattt atacaggttc cagactccac
     4021 gaagccggat gtgcaccctc atatcatgtg gtgcgagggg atattgccac ggccaccgaa
     4081 ggagtgatta taaatgctgc taacagcaaa ggacaacctg gcggaggggt gtgcggagcg
     4141 ctgtataaga aattcccgga aagcttcgat ttacagccga tcgaagtagg aaaagcgcga
     4201 ctggtcaaag gtgcagctaa acatatcatt catgccgtag gaccaaactt caacaaagtt
     4261 tcggaggttg aaggtgacaa acagttggca gaggcttatg agtccatcgc taagattgtc
     4321 aacgataaca attacaagtc agtagcgatt ccactgttgt ccaccggcat cttttccggg
     4381 aacaaagatc gactaaccca atcattgaac catttgctga cagctttaga caccactgat
     4441 gcagatgtag ccatatactg cagggacaag aaatgggaaa tgactctcaa ggaagcagtg
     4501 gctaggagag aagcagtgga ggagatatgc atatccgacg actcttcagt gacagaacct
     4561 gatgcagagc tggtgagggt gcatccgaag agttctttgg ctggaaggaa gggctacagc
     4621 acaagcgatg gcaaaacttt ctcatatttg gaagggacca agtttcacca ggcggccaag
     4681 gatatagcag aaattaatgc catgtggccc gttgcaacgg aggccaatga gcaggtatgc
     4741 atgtatatcc tcggagaaag catgagcagt attaggtcga aatgccccgt cgaagagtcg
     4801 gaagcctcca caccacctag cacgctgcct tgcttgtgca tccatgccat gactccagaa
     4861 agagtacagc gcctaaaagc ctcacgtcca gaacaaatta ctgtgtgctc atcctttcca
     4921 ttgccgaagt atagaatcac tggtgtgcag aagatccaat gctcccagcc tatattgttc
     4981 tcaccgaaag tgcctgcgta tattcatcca aggaagtatc tcgtggaaac accaccggta
     5041 gacgagactc cggagccatc ggcagagaac caatccacag aggggacacc tgaacaacca
     5101 ccacttataa ccgaggatga gaccaggact agaacgcctg agccgatcat catcgaagag
     5161 gaagaagagg atagcataag tttgctgtca gatggcccga cccaccaggt gctgcaagtc
     5221 gaggcagaca ttcacgggcc gccctctgta tctagctcat cctggtccat tcctcatgca
     5281 tccgactttg atgtggacag tttatccata cttgacaccc tggagggagc tagcgtgacc
     5341 agcggggcaa cgtcagccga gactaactct tacttcgcaa agagtatgga gtttctggcg
     5401 cgaccggtgc ctgcgcctcg aacagtattc aggaaccctc cacatcccgc tccgcgcaca
     5461 agaacaccgt cacttgcacc cagcagggcc tgctcgagaa ccagcctagt ttccaccccg
     5521 ccaggcgtga atagggtgat cactagagag gagctcgagg cgcttacccc gtcacgcact
     5581 cctagcaggt cggtctcgag aaccagcctg gtctccaacc cgccaggcgt aaatagggtg
     5641 attacaagag aggagtttga ggcgttcgta gcacaacaac aatgacggtt tgatgcgggt
     5701 gcatacatct tttcctccga caccggtcaa gggcatttac aacaaaaatc agtaaggcaa
     5761 acggtgctat ccgaagtggt gttggagagg accgaattgg agatttcgta tgccccgcgc
     5821 ctcgaccaag aaaaagaaga attactacgc aagaaattac agttaaatcc cacacctgct
     5881 aacagaagca gataccagtc caggaaggtg gagaacatga aagccataac agctagacgt
     5941 attctgcaag gcctagggca ttatttgaag gcagaaggaa aagtggagtg ctaccgaacc
     6001 ctgcatcctg ttcctttgta ttcatctagt gtgaaccgtg ccttttcaag ccccaaggtc
     6061 gcagtggaag cctgtaacgc catgttgaaa gagaactttc cgactgtggc ttcttactgt
     6121 attattccag agtacgatgc ctatttggac atggttgacg gagcttcatg ctgcttagac
     6181 actgccagtt tttgccctgc aaagctgcgc agctttccaa agaaacactc ctatttggaa
     6241 cccacaatac gatcggcagt gccttcagcg atccagaaca cgctccagaa cgtcctggca
     6301 gctgccacaa aaagaaattg caatgtcacg caaatgagag aattgcccgt attggattcg
     6361 gcggccttta atgtggaatg cttcaagaaa tatgcgtgta ataatgaata ttgggaaacg
     6421 tttaaagaaa accccatcag gcttactgaa gaaaacgtgg taaattacat taccaaatta
     6481 aaaggaccaa aagctgctgc tctttttgcg aagacacata atttgaatat gttgcaggac
     6541 ataccaatgg acaggtttgt aatggactta aagagagacg tgaaagtgac tccaggaaca
     6601 aaacatactg aagaacggcc caaggtacag gtgatccagg ctgccgatcc gctagcaaca
     6661 gcgtatctgt gcggaatcca ccgagagctg gttaggagat taaatgcggt cctgcttccg
     6721 aacattcata cactgtttga tatgtcggct gaagactttg acgctattat agccgagcac
     6781 ttccagcctg gggattgtgt tctggaaact gacatcgcgt cgtttgataa aagtgaggac
     6841 gacgccatgg ctctgaccgc gttaatgatt ctggaagact taggtgtgga cgcagagctg
     6901 ttgacgctga ttgaggcggc tttcggcgaa atttcatcaa tacatttgcc cactaaaact
     6961 aaatttaaat tcggagccat gatgaaatct ggaatgttcc tcacactgtt tgtgaacaca
     7021 gtcattaaca ttgtaatcgc aagcagagtg ttgagagaac ggctaaccgg atcaccatgt
     7081 gcagcattca ttggagatga caatatcgtg aaaggagtca aatcggacaa attaatggca
     7141 gacaggtgcg ccacctggtt gaatatggaa gtcaagatta tagatgctgt ggtgggcgag
     7201 aaagcgcctt atttctgtgg agggtttatt ttgtgtgact ccgtgaccgg cacagcgtgc
     7261 cgtgtggcag accccctaaa aaggctgttt aagcttggca aacctctggc agcagacgat
     7321 gaacatgatg atgacaggag aagggcattg catgaagagt caacacgctg gaaccgagtg
     7381 ggtattcttt cagagctgtg caaggcagta gaatcaaggt atgaaaccgt aggaacttcc
     7441 atcatagtta tggccatgac tactctagct agcagtgtta aatcattcag ctacctgaga
     7501 ggggccccta taactctcta cggctaacct gaatggacta cgacatagtc tagtccgcca
     7561 agtctagacc atgagcggcc ggaaggctca gggcaagacc ctgggcgtga acatggtgag
     7621 gcgcggcgtg cgcagcctct ccaacaagat caagcagaag accaagcaga tcggcaacag
     7681 acccggaccg agccggggcg tccaggggtt catcttcttc ttcctgttca acatcctcac
     7741 aggtaagaag atcacggctc acctgaagag gctctggaag atgctggacc ctcgccaggg
     7801 gctcgcggtg ctcagaaagg tgaagcgggt cgtcgcctcc ctgatgcgcg gcctgtcctc
     7861 tcgcaagagg cgctcccacg atgtgctcac cgtccaattc ctcattctgg gaatgctgct
     7921 gatgactggc ggcgtgaccc tggtgcgcaa gaaccgctgg ctgctgctga atgtgaccag
     7981 tgaggacctc gggtaagggc ccctataact ctctacggct aacctgaatg gactacgaca
     8041 tagtctagtc cgccaagtct agagcttacc atgaccgagt acaagcccac ggtgcgcctc
     8101 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     8161 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     8221 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     8281 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     8341 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8401 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8461 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8521 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8581 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc
     8641 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     8701 gaaaggagcg cacgacccca tgatcgctag accatggggt accgagtatg ttacgtgcaa
     8761 aggtgattgt caccccccga aagaccatat tgtgacacac cctcagtatc acgcccaaac
     8821 atttacagcc gcggtgtcaa aaaccgcgtg gacgtggtta acatccctgc tgggaggatc
     8881 agccgtaatt attataattg gcttggtgct ggctactatt gtggccatgt acgtgctgac
     8941 caaccagaaa cataattgaa tacagcagca attggcaagc tgcttacata gaactcgcgg
     9001 cgattggcat gccgccttaa aatttttatt ttattttttc ttttcttttc cgaatcggat
     9061 tttgttttta atatttcaaa aaaaaaaaaa aaaaaaaaaa aacgcgtcga ggggaattaa
     9121 ttcttgaaga cgaaagggcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     9181 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     9241 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     9301 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     9361 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     9421 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9481 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     9541 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9601 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9661 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9721 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9781 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9841 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9901 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9961 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10021 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    10081 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    10141 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    10201 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10261 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10321 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    10381 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    10441 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10501 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10561 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10621 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10681 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10741 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10801 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10861 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcgagctc gatttaggtg
    10921 acactata
//
