LOCUS       40924_49647        6801 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Episomal vector YEp85, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Construction and characterization of centromeric, episomal and 
            GFP-containing vectors for Saccharomyces cerevisiae prototrophic 
            strains
  JOURNAL   J. Biotechnol. 143 (4), 247-254 (2009)
  PUBMED    19683551
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2009) Department of Genetics, Biology of 
            Microorganisms, Anthropology, Evolution, University of Parma, Viale 
            Usberti 11/A, Parma 43100, Italy
REFERENCE   3  (bases 1 to 6801)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6801)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol."; date: "2009"; volume: "143"; issue: "4"; pages: 
            "247-254"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2009) Department of Genetics, Biology of Microorganisms, 
            Anthropology, Evolution, University of Parma, Viale Usberti 11/A, 
            Parma 43100, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(233..289)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(290..306)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(743..2089)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     gene            2243..3599
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(4392..4789)
                     /label=LEU2 promoter
     promoter        4895..4999
                     /label=AmpR promoter
     CDS             5000..5857
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6031..6619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
      541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
      601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
      661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
      721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
      781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
      841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
      901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
      961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
     1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
     1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
     1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
     1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
     1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
     2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
     2101 aagctgtcaa acatgagaat taattctacc ctatgaacat attccatttt gtaatttcgt
     2161 gtcgtttcta ttatgaattt catttataaa gtttatgtac actgtttagc ttgcctcgtc
     2221 cccgccgggt cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga
     2281 cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca
     2341 tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg
     2401 ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc
     2461 ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt
     2521 tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa
     2581 acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc caacatggat
     2641 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2701 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2761 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2821 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2881 atccccggca aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2941 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     3001 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     3061 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     3121 gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     3181 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     3241 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3301 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3361 ttgcagtttc atttgatgct cgatgagttt ttctaatcag tactgacaat aaaaagattc
     3421 ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat
     3481 caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt
     3541 taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc
     3601 tgtcgattcg atactaacgc cgccatccga tatcaccaaa catgttgctg gtgattataa
     3661 taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat
     3721 gttgaacctt caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata
     3781 atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat
     3841 gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt
     3901 gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac
     3961 ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg
     4021 gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt
     4081 gaagttcttt acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt
     4141 taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct tccagcgcct
     4201 catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa tgattttcga
     4261 aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg
     4321 ctgtgatctt gaccaacgtg gtcacctggc aaaacgacga tcttcttagg ggcagacata
     4381 ggggcagaca ttagaatgta tatccttgaa atatatatat atattgctga aatgtaaaag
     4441 gtaagaaaag ttagaataag acgattgcta accacctatt ggaaaaaaca ataggtcctt
     4501 aaataatatt gtcaacttca agtattgtga tgcaagcatt tagtcatgaa cgcttctcta
     4561 ttctatatga aaagccggtt ccggcctctc acctttcctt tttctcccaa tttttcagtt
     4621 gaaaaaggta tatgcgcggc gacctctgaa attaacaaaa aatttccagt catcgaattt
     4681 gtctgtgcga tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct
     4741 aagagattcg aactcttgca tcttacgata cctgagtatt cccacagtta attcttgaag
     4801 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4861 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4921 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4981 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5041 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5101 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5161 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5221 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5281 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5341 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5401 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5461 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5521 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5581 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5641 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5701 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5761 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5821 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5881 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5941 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6001 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6061 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6661 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6721 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6781 agtgagcgag gaagcggaag a
//
