LOCUS       40924_49637        8433 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a LEU2 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp368.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 8433)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8433)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             72..3119
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3715..4806)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MFVPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWTLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEALRYPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPGFAKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4807..5211)
                     /label=LEU2 promoter
     rep_origin      complement(5212..6194)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6476..6580
                     /label=AmpR promoter
     CDS             6581..7438
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7612..8200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattccca
       61 gcttcgatcc cgccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      121 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      181 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
      241 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
      301 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa
      361 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
      421 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
      481 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
      541 acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc
      601 tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc
      661 ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg
      721 atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag
      781 ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg
      841 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg
      901 gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg
      961 aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg
     1021 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc
     1081 tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg
     1141 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact
     1201 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc
     1261 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
     1321 atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg
     1381 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc
     1441 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg
     1501 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg
     1561 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc
     1621 tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta
     1681 acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg
     1741 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc
     1801 cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga
     1861 acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc
     1921 agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt
     1981 tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg
     2041 caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg
     2101 aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga
     2161 acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg
     2221 aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg
     2281 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct
     2341 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt
     2401 tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
     2461 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc
     2521 agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc
     2581 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa
     2641 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
     2701 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
     2761 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
     2821 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
     2881 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
     2941 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
     3001 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
     3061 cggcggaatt acagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
     3121 aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact
     3181 atttcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
     3241 tccggtacgt cgttaaggcc gtttctgaca gagtaaaatt cttgagggaa ctttcaccat
     3301 tatggaaaat ggttcaagaa ggtattgact taaactccat caaatggtca ggtcatttga
     3361 gtgtttttta tttgttgtat tttttttttt ttagagaaaa tcctccaata tcaaattagg
     3421 gattcgtagt ttcatgattt tctgttatac ctaacttttt gtgtggtgcc ctcctccttg
     3481 tcaatattaa tgttaaagtg caattctctt tccttatcac gttgagccat tagtatcaat
     3541 ttgcttacct gtattccttt agctatcctc ctttttctcc ttcttgataa atgtatgtag
     3601 attgcgtttg tctaccctat gaacatattc cattttgtaa tttcgtgtcg tttctattat
     3661 gaatttcatt tataaagttt atgtacaaat atcataaaaa agagaatctt tttaagcaag
     3721 gattttctta acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc
     3781 taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt
     3841 accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc
     3901 gatagggtca accttattct tggcaaatcc gggagcagaa ccgtggcatg gttcgtacaa
     3961 accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa
     4021 ggaacctgga taacgtaagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat
     4081 tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa
     4141 ttgatgttga accttcaatg tagggaattc gttcttgatg gtttcctcca cagtttttct
     4201 ccataatctt gaagaggcca aaacattagc tttatccaag gtccaaatag gcaatggtgg
     4261 ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt
     4321 gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta
     4381 aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt
     4441 gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta
     4501 caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac taccggtacc
     4561 ccattttgga ccacccacag cacctaacaa aacggcatca accttcttgg aggcttccag
     4621 tgcctcatct ggaagtggga cacctgtagc atcgatagca gcaccaccaa ttaaatgatt
     4681 ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc
     4741 ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggaac
     4801 aaacattaga atggtatatc cttgaaatat atatatatat tgctgaaatg taaaaggtaa
     4861 gaaaagttag aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa
     4921 ataatattgt caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt
     4981 ctatatgaaa agccggttcc ggcctctcac ctttcctttt tctcccaatt tttcagttga
     5041 aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa aatttccagt catcgaattt
     5101 gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg
     5161 ctaagagatt cgaactcttg catcttacga tacctgagta ttcccacagt taacgaagca
     5221 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     5281 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     5341 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     5401 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     5461 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     5521 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     5581 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     5641 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     5701 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     5761 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     5821 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     5881 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     5941 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     6001 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     6061 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     6121 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     6181 cctatacttt ctagtatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6241 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6301 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6361 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6421 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6481 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6541 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6601 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6661 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6721 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6781 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6841 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6901 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6961 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7021 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7081 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7141 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7201 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7261 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7321 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7381 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7441 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7501 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7741 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7981 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     8041 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8221 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8281 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8341 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8401 gcctctcccc gcgcgttggc cgattcattc ccg
//
