LOCUS       40924_49627        8432 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a LEU2 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp367.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 8432)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8432)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             71..3118
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3714..4805)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MFVPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWTLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEALRYPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPGFAKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4806..5210)
                     /label=LEU2 promoter
     rep_origin      complement(5211..6193)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6475..6579
                     /label=AmpR promoter
     CDS             6580..7437
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7611..8199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattccca
       61 gcttgctccc gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      121 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      181 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
      241 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
      301 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac
      361 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
      421 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
      481 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga
      541 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
      601 cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg gaagatcagg atatgtggcg
      661 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga
      721 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt
      781 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga
      841 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg
      901 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga
      961 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga
     1021 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct
     1081 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg
     1141 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt
     1201 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
     1261 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
     1321 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
     1381 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca
     1441 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt
     1501 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc
     1561 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct
     1621 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa
     1681 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
     1741 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
     1801 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
     1861 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca
     1921 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
     1981 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
     2041 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
     2101 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
     2161 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga
     2221 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga
     2281 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt
     2341 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt
     2401 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa
     2461 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca
     2521 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca
     2581 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat
     2641 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct
     2701 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga
     2761 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga
     2821 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt
     2881 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca
     2941 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct
     3001 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc
     3061 ggcggaatta cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata
     3121 ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta
     3181 tttcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagat
     3241 ccggtacgtc gttaaggccg tttctgacag agtaaaattc ttgagggaac tttcaccatt
     3301 atggaaaatg gttcaagaag gtattgactt aaactccatc aaatggtcag gtcatttgag
     3361 tgttttttat ttgttgtatt tttttttttt tagagaaaat cctccaatat caaattaggg
     3421 attcgtagtt tcatgatttt ctgttatacc taactttttg tgtggtgccc tcctccttgt
     3481 caatattaat gttaaagtgc aattctcttt ccttatcacg ttgagccatt agtatcaatt
     3541 tgcttacctg tattccttta gctatcctcc tttttctcct tcttgataaa tgtatgtaga
     3601 ttgcgtttgt ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg
     3661 aatttcattt ataaagttta tgtacaaata tcataaaaaa gagaatcttt ttaagcaagg
     3721 attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct
     3781 aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta
     3841 ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg
     3901 atagggtcaa ccttattctt ggcaaatccg ggagcagaac cgtggcatgg ttcgtacaaa
     3961 ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag
     4021 gaacctggat aacgtaaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt
     4081 ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat
     4141 tgatgttgaa ccttcaatgt agggaattcg ttcttgatgg tttcctccac agtttttctc
     4201 cataatcttg aagaggccaa aacattagct ttatccaagg tccaaatagg caatggtggc
     4261 tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc tggaacggtg
     4321 tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt accaaagtaa
     4381 atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg
     4441 attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa gttggcgtac
     4501 aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact accggtaccc
     4561 cattttggac cacccacagc acctaacaaa acggcatcaa ccttcttgga ggcttccagt
     4621 gcctcatctg gaagtgggac acctgtagca tcgatagcag caccaccaat taaatgattt
     4681 tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac cttaatggct
     4741 tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt cttaggaaca
     4801 aacattagaa tggtatatcc ttgaaatata tatatatatt gctgaaatgt aaaaggtaag
     4861 aaaagttaga aagtaagacg attgctaacc acctattgga aaaaacaata ggtccttaaa
     4921 taatattgtc aacttcaagt attgtgatgc aagcatttag tcatgaacgc ttctctattc
     4981 tatatgaaaa gccggttccg gcctctcacc tttccttttt ctcccaattt ttcagttgaa
     5041 aaaggtatat gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg
     5101 attctgtgcg atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc
     5161 taagagattc gaactcttgc atcttacgat acctgagtat tcccacagtt aacgaagcat
     5221 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     5281 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
     5341 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     5401 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca
     5461 acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc
     5521 taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg
     5581 ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt
     5641 ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc
     5701 gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg
     5761 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga
     5821 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt
     5881 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact
     5941 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt
     6001 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag
     6061 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt
     6121 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc
     6181 ctatactttc tagtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6241 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     6301 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6361 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6421 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6541 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6601 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6661 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6721 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6781 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6841 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6901 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6961 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7021 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7081 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7141 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7201 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7261 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7321 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7381 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7441 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7501 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7561 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7741 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     7801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7981 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8401 cctctccccg cgcgttggcc gattcattcc cg
//
