LOCUS       40924_49622        8425 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a LEU2 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp367R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 8425)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             64..3111
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3707..4798)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MFVPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWTLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEALRYPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPGFAKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4799..5203)
                     /label=LEU2 promoter
     rep_origin      complement(5204..6186)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6468..6572
                     /label=AmpR promoter
     CDS             6573..7430
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7604..8192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgct
       61 cccgccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      181 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
      241 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc
      301 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc
      361 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca
      421 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt
      481 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt
      541 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg
      601 atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc
      661 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat
      721 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg
      781 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag
      841 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc
      901 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg
      961 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa
     1021 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc
     1081 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc
     1141 atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc
     1201 gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc
     1261 ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg
     1321 accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc
     1381 gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct
     1441 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat
     1501 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg
     1561 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta
     1621 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt
     1681 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc
     1741 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg
     1801 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg
     1861 gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt
     1921 ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat
     1981 agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt
     2041 gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg
     2101 cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc
     2161 gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc
     2221 agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat
     2281 ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca
     2341 cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt
     2401 gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg
     2461 gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg
     2521 gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg
     2581 aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt
     2641 accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc
     2701 cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat
     2761 cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat
     2821 accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat
     2881 ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa
     2941 ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc
     3001 gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa
     3061 ttacagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
     3121 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttcgcc
     3181 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gatccggtac
     3241 gtcgttaagg ccgtttctga cagagtaaaa ttcttgaggg aactttcacc attatggaaa
     3301 atggttcaag aaggtattga cttaaactcc atcaaatggt caggtcattt gagtgttttt
     3361 tatttgttgt attttttttt ttttagagaa aatcctccaa tatcaaatta gggattcgta
     3421 gtttcatgat tttctgttat acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt
     3481 aatgttaaag tgcaattctc tttccttatc acgttgagcc attagtatca atttgcttac
     3541 ctgtattcct ttagctatcc tcctttttct ccttcttgat aaatgtatgt agattgcgtt
     3601 tgtctaccct atgaacatat tccattttgt aatttcgtgt cgtttctatt atgaatttca
     3661 tttataaagt ttatgtacaa atatcataaa aaagagaatc tttttaagca aggattttct
     3721 taacttcttc ggcgacagca tcaccgactt cggtggtact gttggaacca cctaaatcac
     3781 cagttctgat acctgcatcc aaaacctttt taactgcatc ttcaatggcc ttaccttctt
     3841 caggcaagtt caatgacaat ttcaacatca ttgcagcaga caagatagtg gcgatagggt
     3901 caaccttatt cttggcaaat ccgggagcag aaccgtggca tggttcgtac aaaccaaatg
     3961 cggtgttctt gtctggcaaa gaggccaagg acgcagatgg caacaaaccc aaggaacctg
     4021 gataacgtaa ggcttcatcg gagatgatat caccaaacat gttgctggtg attataatac
     4081 catttaggtg ggttgggttc ttaactagga tcatggcggc agaatcaatc aattgatgtt
     4141 gaaccttcaa tgtagggaat tcgttcttga tggtttcctc cacagttttt ctccataatc
     4201 ttgaagaggc caaaacatta gctttatcca aggtccaaat aggcaatggt ggctcatgtt
     4261 gtagggccat gaaagcggcc attcttgtga ttctttgcac ttctggaacg gtgtattgtt
     4321 cactatccca agcgacacca tcaccatcgt cttcctttct cttaccaaag taaatacctc
     4381 ccactaattc tctgacaaca acgaagtcag tacctttagc aaattgtggc ttgattggag
     4441 ataagtctaa aagagagtcg gatgcaaagt tacatggtct taagttggcg tacaattgaa
     4501 gttctttacg gatttttagt aaaccttgtt caggtctaac actaccggta ccccattttg
     4561 gaccacccac agcacctaac aaaacggcat caaccttctt ggaggcttcc agtgcctcat
     4621 ctggaagtgg gacacctgta gcatcgatag cagcaccacc aattaaatga ttttcgaaat
     4681 cgaacttgac attggaacga acatcagaaa tagctttaag aaccttaatg gcttcggctg
     4741 tgatttcttg accaacgtgg tcacctggca aaacgacgat cttcttagga acaaacatta
     4801 gaatggtata tccttgaaat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt
     4861 agaaagtaag acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt
     4921 gtcaacttca agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga
     4981 aaagccggtt ccggcctctc acctttcctt tttctcccaa tttttcagtt gaaaaaggta
     5041 tatgcgtcag gcgacctctg aaattaacaa aaaatttcca gtcatcgaat ttgattctgt
     5101 gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt tgctaagaga
     5161 ttcgaactct tgcatcttac gatacctgag tattcccaca gttaacgaag catctgtgct
     5221 tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     5281 ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg
     5341 tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
     5401 ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga
     5461 atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa
     5521 gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt
     5581 tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc
     5641 ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca
     5701 ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt
     5761 tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt
     5821 cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg
     5881 attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata
     5941 aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg
     6001 taggttatat agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt
     6061 tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt
     6121 tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact
     6181 ttctagtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     6241 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6301 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6361 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6421 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6481 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6541 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6601 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6661 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6721 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6781 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6841 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6901 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6961 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7021 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7081 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7141 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7201 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7261 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7321 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7381 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7441 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7501 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7561 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7621 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7681 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7741 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7801 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7861 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7921 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7981 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8041 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8101 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8161 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8221 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8281 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8341 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8401 ccgcgcgttg gccgattcat tcccg
//
