LOCUS       40924_49612        8427 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a LEU2 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp366R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 8427)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8427)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             66..3113
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3709..4800)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MFVPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWTLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEALRYPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPGFAKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4801..5205)
                     /label=LEU2 promoter
     rep_origin      complement(5206..6188)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6470..6574
                     /label=AmpR promoter
     CDS             6575..7432
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7606..8194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgcg
       61 atcccgccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      121 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      181 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
      241 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
      301 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc
      361 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
      421 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
      481 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc
      541 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg
      601 tgatggtgct gcgttggagt gacggcagtt atctggaaga tcaggatatg tggcggatga
      661 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc
      721 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga
      781 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc
      841 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg
      901 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc
      961 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag
     1021 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg
     1081 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg
     1141 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg
     1201 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg
     1261 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc
     1321 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc
     1381 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg
     1441 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt
     1501 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg
     1561 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc
     1621 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc
     1681 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct
     1741 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt
     1801 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc
     1861 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt
     1921 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc
     1981 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg
     2041 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac
     2101 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga
     2161 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc
     2221 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg
     2281 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt
     2341 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc
     2401 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct
     2461 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
     2521 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
     2581 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
     2641 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
     2701 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
     2761 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
     2821 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt
     2881 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc
     2941 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata
     3001 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg
     3061 aattacagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat
     3121 aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttcg
     3181 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagatccggt
     3241 acgtcgttaa ggccgtttct gacagagtaa aattcttgag ggaactttca ccattatgga
     3301 aaatggttca agaaggtatt gacttaaact ccatcaaatg gtcaggtcat ttgagtgttt
     3361 tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat tagggattcg
     3421 tagtttcatg attttctgtt atacctaact ttttgtgtgg tgccctcctc cttgtcaata
     3481 ttaatgttaa agtgcaattc tctttcctta tcacgttgag ccattagtat caatttgctt
     3541 acctgtattc ctttagctat cctccttttt ctccttcttg ataaatgtat gtagattgcg
     3601 tttgtctacc ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt
     3661 catttataaa gtttatgtac aaatatcata aaaaagagaa tctttttaag caaggatttt
     3721 cttaacttct tcggcgacag catcaccgac ttcggtggta ctgttggaac cacctaaatc
     3781 accagttctg atacctgcat ccaaaacctt tttaactgca tcttcaatgg ccttaccttc
     3841 ttcaggcaag ttcaatgaca atttcaacat cattgcagca gacaagatag tggcgatagg
     3901 gtcaacctta ttcttggcaa atccgggagc agaaccgtgg catggttcgt acaaaccaaa
     3961 tgcggtgttc ttgtctggca aagaggccaa ggacgcagat ggcaacaaac ccaaggaacc
     4021 tggataacgt aaggcttcat cggagatgat atcaccaaac atgttgctgg tgattataat
     4081 accatttagg tgggttgggt tcttaactag gatcatggcg gcagaatcaa tcaattgatg
     4141 ttgaaccttc aatgtaggga attcgttctt gatggtttcc tccacagttt ttctccataa
     4201 tcttgaagag gccaaaacat tagctttatc caaggtccaa ataggcaatg gtggctcatg
     4261 ttgtagggcc atgaaagcgg ccattcttgt gattctttgc acttctggaa cggtgtattg
     4321 ttcactatcc caagcgacac catcaccatc gtcttccttt ctcttaccaa agtaaatacc
     4381 tcccactaat tctctgacaa caacgaagtc agtaccttta gcaaattgtg gcttgattgg
     4441 agataagtct aaaagagagt cggatgcaaa gttacatggt cttaagttgg cgtacaattg
     4501 aagttcttta cggattttta gtaaaccttg ttcaggtcta acactaccgg taccccattt
     4561 tggaccaccc acagcaccta acaaaacggc atcaaccttc ttggaggctt ccagtgcctc
     4621 atctggaagt gggacacctg tagcatcgat agcagcacca ccaattaaat gattttcgaa
     4681 atcgaacttg acattggaac gaacatcaga aatagcttta agaaccttaa tggcttcggc
     4741 tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg atcttcttag gaacaaacat
     4801 tagaatggta tatccttgaa atatatatat atattgctga aatgtaaaag gtaagaaaag
     4861 ttagaaagta agacgattgc taaccaccta ttggaaaaaa caataggtcc ttaaataata
     4921 ttgtcaactt caagtattgt gatgcaagca tttagtcatg aacgcttctc tattctatat
     4981 gaaaagccgg ttccggcctc tcacctttcc tttttctccc aatttttcag ttgaaaaagg
     5041 tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc cagtcatcga atttgattct
     5101 gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa aaaaataatg gttgctaaga
     5161 gattcgaact cttgcatctt acgatacctg agtattccca cagttaacga agcatctgtg
     5221 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     5281 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
     5341 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     5401 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
     5461 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
     5521 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
     5581 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
     5641 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
     5701 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
     5761 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
     5821 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
     5881 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
     5941 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
     6001 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
     6061 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt
     6121 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
     6181 ctttctagta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     6241 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     6301 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     6361 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6421 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6541 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6601 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6661 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6721 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6781 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6841 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6901 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6961 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7021 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7081 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7141 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7201 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7261 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7321 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7381 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7441 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7741 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7981 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8221 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8281 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8341 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8401 ccccgcgcgt tggccgattc attcccg
//
