LOCUS       YEp365.        8405 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a LEU2 marker for construction of lacZ 
            fusions. For other reading frames, use YEp363 or YEp364.
ACCESSION   .
VERSION     .
KEYWORDS    YEp365.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 8405)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8405)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             44..3091
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3687..4778)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(4779..5183)
                     /label=LEU2 promoter
     rep_origin      complement(5184..6166)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6448..6552
                     /label=AmpR promoter
     CDS             6553..7410
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7584..8172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcccgg ggatccgtcg acctgcagcc aagcttcgat cccgccgtcg ttttacaacg
       61 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      121 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      181 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
      241 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
      301 cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt
      361 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
      421 gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg
      481 gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct
      541 gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga
      601 cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc
      661 gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga
      721 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta
      781 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc
      841 tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct
      901 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt
      961 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg
     1021 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg
     1081 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt
     1141 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
     1201 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
     1261 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
     1321 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
     1381 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg
     1441 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac
     1501 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc
     1561 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct
     1621 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg
     1681 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca
     1741 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg
     1801 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc
     1861 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
     1921 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
     1981 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
     2041 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
     2101 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca
     2161 catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc
     2221 gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa
     2281 taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa
     2341 aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat
     2401 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc
     2461 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc
     2521 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg
     2581 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc
     2641 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga
     2701 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc
     2761 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag
     2821 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
     2881 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
     2941 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
     3001 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga gcgccggtcg
     3061 ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc
     3121 cgtatttcgc gtaaggaaat ccattatgta ctatttcgcc tgatgcggta ttttctcctt
     3181 acgcatctgt gcggtatttc acaccgcata gatccggtac gtcgttaagg ccgtttctga
     3241 cagagtaaaa ttcttgaggg aactttcacc attatggaaa atggttcaag aaggtattga
     3301 cttaaactcc atcaaatggt caggtcattt gagtgttttt tatttgttgt attttttttt
     3361 ttttagagaa aatcctccaa tatcaaatta gggattcgta gtttcatgat tttctgttat
     3421 acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctc
     3481 tttccttatc acgttgagcc attagtatca atttgcttac ctgtattcct ttagctatcc
     3541 tcctttttct ccttcttgat aaatgtatgt agattgcgtt tgtctaccct atgaacatat
     3601 tccattttgt aatttcgtgt cgtttctatt atgaatttca tttataaagt ttatgtacaa
     3661 atatcataaa aaagagaatc tttttaagca aggattttct taacttcttc ggcgacagca
     3721 tcaccgactt cggtggtact gttggaacca cctaaatcac cagttctgat acctgcatcc
     3781 aaaacctttt taactgcatc ttcaatggcc ttaccttctt caggcaagtt caatgacaat
     3841 ttcaacatca ttgcagcaga caagatagtg gcgatagggt caaccttatt cttggcaaat
     3901 ccgggagcag aaccgtggca tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa
     3961 gaggccaagg acgcagatgg caacaaaccc aaggaacctg gataacgtaa ggcttcatcg
     4021 gagatgatat caccaaacat gttgctggtg attataatac catttaggtg ggttgggttc
     4081 ttaactagga tcatggcggc agaatcaatc aattgatgtt gaaccttcaa tgtagggaat
     4141 tcgttcttga tggtttcctc cacagttttt ctccataatc ttgaagaggc caaaacatta
     4201 gctttatcca aggtccaaat aggcaatggt ggctcatgtt gtagggccat gaaagcggcc
     4261 attcttgtga ttctttgcac ttctggaacg gtgtattgtt cactatccca agcgacacca
     4321 tcaccatcgt cttcctttct cttaccaaag taaatacctc ccactaattc tctgacaaca
     4381 acgaagtcag tacctttagc aaattgtggc ttgattggag ataagtctaa aagagagtcg
     4441 gatgcaaagt tacatggtct taagttggcg tacaattgaa gttctttacg gatttttagt
     4501 aaaccttgtt caggtctaac actaccggta ccccattttg gaccacccac agcacctaac
     4561 aaaacggcat caaccttctt ggaggcttcc agtgcctcat ctggaagtgg gacacctgta
     4621 gcatcgatag cagcaccacc aattaaatga ttttcgaaat cgaacttgac attggaacga
     4681 acatcagaaa tagctttaag aaccttaatg gcttcggctg tgatttcttg accaacgtgg
     4741 tcacctggca aaacgacgat cttcttagga acaaacatta gaatggtata tccttgaaat
     4801 atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta
     4861 accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga
     4921 tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcctctc
     4981 acctttcctt tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag gcgacctctg
     5041 aaattaacaa aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc ccctgtgtgt
     5101 tctcgttatg ttgaggaaaa aaataatggt tgctaagaga ttcgaactct tgcatcttac
     5161 gatacctgag tattcccaca gttaacgaag catctgtgct tcattttgta gaacaaaaat
     5221 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
     5281 aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca
     5341 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     5401 acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt
     5461 ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt
     5521 tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta
     5581 aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca
     5641 cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat
     5701 ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc
     5761 gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat
     5821 actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc
     5881 ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt
     5941 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag
     6001 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa
     6061 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
     6121 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagtatg gtgcactctc
     6181 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     6241 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6301 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     6361 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6421 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6481 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6541 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6601 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6661 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6721 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6781 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6841 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6901 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6961 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7021 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7081 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7141 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7201 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7261 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7321 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7381 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7441 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7501 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7561 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7621 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7681 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7741 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7801 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7861 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7921 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     7981 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8041 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8101 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8161 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8221 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8281 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8341 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     8401 tcccg
//
