LOCUS       YEp363.        8406 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a LEU2 marker for construction of lacZ 
            fusions. For other reading frames, use YEp364 or YEp365.
ACCESSION   .
VERSION     .
KEYWORDS    YEp363
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 8406)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8406)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             45..3092
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3688..4779)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(4780..5184)
                     /label=LEU2 promoter
     rep_origin      complement(5185..6167)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6449..6553
                     /label=AmpR promoter
     CDS             6554..7411
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7585..8173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcccgg ggatccgtcg acctgcagcc aagcttgcga tcccgccgtc gttttacaac
       61 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      181 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
      241 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
      301 acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt
      361 ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct
      421 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
      481 ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc
      541 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg
      601 acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct
      661 cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg
      721 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact
      781 acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
      841 ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc
      901 tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
      961 ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
     1021 gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag
     1081 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
     1141 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
     1201 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
     1261 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
     1321 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
     1381 tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc
     1441 gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca
     1501 ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
     1561 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc
     1621 tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact
     1681 ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc
     1741 agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg
     1801 gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc
     1861 cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg
     1921 ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc
     1981 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg
     2041 ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc
     2101 aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc
     2161 acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg
     2221 cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta
     2281 ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata
     2341 aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
     2401 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg
     2461 cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg
     2521 cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc
     2581 ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg
     2641 cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag
     2701 agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg
     2761 cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
     2821 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
     2881 gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc
     2941 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
     3001 ttggtggcga cgactcctgg agcccgtcag tatcggcgga attacagctg agcgccggtc
     3061 gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc
     3121 ccgtatttcg cgtaaggaaa tccattatgt actatttcgc ctgatgcggt attttctcct
     3181 tacgcatctg tgcggtattt cacaccgcat agatccggta cgtcgttaag gccgtttctg
     3241 acagagtaaa attcttgagg gaactttcac cattatggaa aatggttcaa gaaggtattg
     3301 acttaaactc catcaaatgg tcaggtcatt tgagtgtttt ttatttgttg tatttttttt
     3361 tttttagaga aaatcctcca atatcaaatt agggattcgt agtttcatga ttttctgtta
     3421 tacctaactt tttgtgtggt gccctcctcc ttgtcaatat taatgttaaa gtgcaattct
     3481 ctttccttat cacgttgagc cattagtatc aatttgctta cctgtattcc tttagctatc
     3541 ctcctttttc tccttcttga taaatgtatg tagattgcgt ttgtctaccc tatgaacata
     3601 ttccattttg taatttcgtg tcgtttctat tatgaatttc atttataaag tttatgtaca
     3661 aatatcataa aaaagagaat ctttttaagc aaggattttc ttaacttctt cggcgacagc
     3721 atcaccgact tcggtggtac tgttggaacc acctaaatca ccagttctga tacctgcatc
     3781 caaaaccttt ttaactgcat cttcaatggc cttaccttct tcaggcaagt tcaatgacaa
     3841 tttcaacatc attgcagcag acaagatagt ggcgataggg tcaaccttat tcttggcaaa
     3901 tccgggagca gaaccgtggc atggttcgta caaaccaaat gcggtgttct tgtctggcaa
     3961 agaggccaag gacgcagatg gcaacaaacc caaggaacct ggataacgta aggcttcatc
     4021 ggagatgata tcaccaaaca tgttgctggt gattataata ccatttaggt gggttgggtt
     4081 cttaactagg atcatggcgg cagaatcaat caattgatgt tgaaccttca atgtagggaa
     4141 ttcgttcttg atggtttcct ccacagtttt tctccataat cttgaagagg ccaaaacatt
     4201 agctttatcc aaggtccaaa taggcaatgg tggctcatgt tgtagggcca tgaaagcggc
     4261 cattcttgtg attctttgca cttctggaac ggtgtattgt tcactatccc aagcgacacc
     4321 atcaccatcg tcttcctttc tcttaccaaa gtaaatacct cccactaatt ctctgacaac
     4381 aacgaagtca gtacctttag caaattgtgg cttgattgga gataagtcta aaagagagtc
     4441 ggatgcaaag ttacatggtc ttaagttggc gtacaattga agttctttac ggatttttag
     4501 taaaccttgt tcaggtctaa cactaccggt accccatttt ggaccaccca cagcacctaa
     4561 caaaacggca tcaaccttct tggaggcttc cagtgcctca tctggaagtg ggacacctgt
     4621 agcatcgata gcagcaccac caattaaatg attttcgaaa tcgaacttga cattggaacg
     4681 aacatcagaa atagctttaa gaaccttaat ggcttcggct gtgatttctt gaccaacgtg
     4741 gtcacctggc aaaacgacga tcttcttagg aacaaacatt agaatggtat atccttgaaa
     4801 tatatatata tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct
     4861 aaccacctat tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg
     4921 atgcaagcat ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct
     4981 cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct
     5041 gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg
     5101 ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta
     5161 cgatacctga gtattcccac agttaacgaa gcatctgtgc ttcattttgt agaacaaaaa
     5221 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     5281 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
     5341 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     5401 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
     5461 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
     5521 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
     5581 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
     5641 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
     5701 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
     5761 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
     5821 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
     5881 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
     5941 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
     6001 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
     6061 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
     6121 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagtat ggtgcactct
     6181 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     6241 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     6301 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     6361 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     6421 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6481 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6541 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6601 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6661 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6721 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6781 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6841 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6901 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6961 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7021 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7081 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7141 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7201 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7261 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7321 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7381 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7441 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7501 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7561 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7621 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7681 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7741 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7801 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7861 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7921 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     7981 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8041 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8101 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8161 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8221 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8281 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8341 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     8401 ttcccg
//
