LOCUS       40924_49592        7971 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp358.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7971)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7971)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             72..3119
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3320..4120)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4121..4331)
                     /label=URA3 promoter
     rep_origin      complement(4568..5732)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6014..6118
                     /label=AmpR promoter
     CDS             6119..6976
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7150..7738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattccca
       61 gcttcgatcc cgccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      121 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      181 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
      241 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
      301 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa
      361 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
      421 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
      481 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
      541 acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc
      601 tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc
      661 ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg
      721 atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag
      781 ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg
      841 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg
      901 gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg
      961 aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg
     1021 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc
     1081 tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg
     1141 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact
     1201 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc
     1261 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
     1321 atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg
     1381 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc
     1441 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg
     1501 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg
     1561 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc
     1621 tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta
     1681 acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg
     1741 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc
     1801 cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga
     1861 acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc
     1921 agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt
     1981 tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg
     2041 caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg
     2101 aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga
     2161 acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg
     2221 aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg
     2281 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct
     2341 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt
     2401 tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
     2461 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc
     2521 agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc
     2581 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa
     2641 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
     2701 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
     2761 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
     2821 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
     2881 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
     2941 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
     3001 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
     3061 cggcggaatt acagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
     3121 aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact
     3181 atttcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg
     3241 gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta
     3301 cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg
     3361 cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc
     3421 caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac
     3481 ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac
     3541 cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc
     3601 tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg
     3661 acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct
     3721 tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt
     3781 ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa
     3841 attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa
     3901 ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt
     3961 tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag
     4021 cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag
     4081 gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt cgttttttgt
     4141 tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg
     4201 tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac
     4261 cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga
     4321 attgaattga aaagctactt gttacccatc attgaatttt gaacatccga acctgggagt
     4381 tttccctgaa acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac
     4441 ggaagacaat gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat
     4501 cttgcacgtc gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat
     4561 ctttgttaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct
     4621 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc
     4681 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga
     4741 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     4801 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc
     4861 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc
     4921 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct
     4981 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg
     5041 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta
     5101 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat
     5161 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat
     5221 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt
     5281 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
     5341 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
     5401 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt
     5461 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
     5521 aaagcgctct gaagttccta tactttctag ctagagaata ggaacttcgg aataggaact
     5581 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     5641 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     5701 acggcatagt gcgtgtttat gcttaaatgc gttatggtgc actctcagta caatctgctc
     5761 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5821 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5881 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5941 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6001 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6061 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6121 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6181 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6241 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6301 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6361 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6421 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6481 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6541 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6601 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6661 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6721 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6781 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6841 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6901 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6961 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7021 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7081 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7141 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7201 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7261 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7321 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7381 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7441 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7501 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     7561 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7621 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7681 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7741 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7801 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7861 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7921 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattccc g
//
