LOCUS       40924_49587        7964 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp358R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7964)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7964)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             65..3112
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3313..4113)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4114..4324)
                     /label=URA3 promoter
     rep_origin      complement(4561..5725)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6007..6111
                     /label=AmpR promoter
     CDS             6112..6969
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7143..7731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttcga
       61 tcccgccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
      241 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
      301 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc
      361 cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac
      421 atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt
      481 taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg
      541 tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt
      601 gatggtgctg cgttggagtg acggcagtta tctggaagat caggatatgt ggcggatgag
      661 cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca
      721 tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat
      781 gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca
      841 ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc
      901 cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc
      961 gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga
     1021 agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg
     1081 caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt
     1141 catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc
     1201 cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg
     1261 cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct
     1321 gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg
     1381 cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc
     1441 taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta
     1501 tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt
     1561 ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct
     1621 acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct
     1681 tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt
     1741 cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc
     1801 ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct
     1861 ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt
     1921 tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca
     1981 tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg
     2041 tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc
     2101 gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac
     2161 cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct
     2221 cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga
     2281 tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc
     2341 acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg
     2401 tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg
     2461 ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac
     2521 ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg
     2581 gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat
     2641 taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg
     2701 ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta
     2761 tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta
     2821 taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta
     2881 tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca
     2941 actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat
     3001 cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga
     3061 attacagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
     3121 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttcgc
     3181 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa
     3241 ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa
     3301 tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct
     3361 gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat
     3421 aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc
     3481 gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc
     3541 tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc
     3601 aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc
     3661 tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc
     3721 gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat
     3781 tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct
     3841 gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc
     3901 ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc
     3961 taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa
     4021 gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt
     4081 agcagcacgt tccttatatg tagctttcga catgatttat cttcgttttt tgttctgtgc
     4141 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat
     4201 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
     4261 aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat
     4321 tgaaaagcta cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct
     4381 gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac
     4441 aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac
     4501 gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt
     4561 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
     4621 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
     4681 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
     4741 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     4801 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     4861 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     4921 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     4981 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     5041 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     5101 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     5161 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     5221 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
     5281 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
     5341 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     5401 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
     5461 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
     5521 tctgaagttc ctatactttc tagctagaga ataggaactt cggaatagga acttcaaagc
     5581 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt
     5641 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat
     5701 agtgcgtgtt tatgcttaaa tgcgttatgg tgcactctca gtacaatctg ctctgatgcc
     5761 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5821 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5881 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     5941 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6001 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6061 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6121 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6181 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6241 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6301 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6361 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6421 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6481 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6541 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6601 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6661 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6721 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6781 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6841 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6901 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6961 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7021 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7081 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7141 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7201 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7261 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7321 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7381 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7441 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7501 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     7561 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7621 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7681 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7741 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7801 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7861 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7921 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt cccg
//
