LOCUS       40924_49582        7970 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp357.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7970)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7970)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             71..3118
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3319..4119)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4120..4330)
                     /label=URA3 promoter
     rep_origin      complement(4567..5731)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6013..6117
                     /label=AmpR promoter
     CDS             6118..6975
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7149..7737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattccca
       61 gcttgctccc gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      121 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      181 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
      241 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
      301 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac
      361 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
      421 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
      481 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga
      541 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
      601 cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg gaagatcagg atatgtggcg
      661 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga
      721 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt
      781 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga
      841 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg
      901 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga
      961 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga
     1021 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct
     1081 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg
     1141 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt
     1201 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
     1261 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
     1321 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
     1381 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca
     1441 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt
     1501 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc
     1561 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct
     1621 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa
     1681 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
     1741 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
     1801 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
     1861 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca
     1921 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
     1981 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
     2041 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
     2101 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
     2161 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga
     2221 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga
     2281 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt
     2341 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt
     2401 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa
     2461 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca
     2521 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca
     2581 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat
     2641 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct
     2701 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga
     2761 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga
     2821 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt
     2881 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca
     2941 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct
     3001 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc
     3061 ggcggaatta cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata
     3121 ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta
     3181 tttcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg
     3241 taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac
     3301 ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc
     3361 ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc
     3421 aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc
     3481 caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc
     3541 ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct
     3601 cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga
     3661 caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt
     3721 caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc
     3781 tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa
     3841 ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac
     3901 tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt
     3961 gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc
     4021 acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa caggactagg
     4081 atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc gttttttgtt
     4141 ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt
     4201 atatatacca atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc
     4261 gaatcaaaaa aatttcaaag aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa
     4321 ttgaattgaa aagctacttg ttacccatca ttgaattttg aacatccgaa cctgggagtt
     4381 ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta gcgctttacg
     4441 gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc ataggtaatc
     4501 ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca atagcatatc
     4561 tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
     4621 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
     4681 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
     4741 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     4801 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
     4861 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
     4921 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
     4981 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
     5041 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
     5101 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
     5161 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
     5221 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
     5281 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
     5341 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     5401 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
     5461 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
     5521 aagcgctctg aagttcctat actttctagc tagagaatag gaacttcgga ataggaactt
     5581 caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct
     5641 cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa
     5701 cggcatagtg cgtgtttatg cttaaatgcg ttatggtgca ctctcagtac aatctgctct
     5761 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     5821 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5881 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5941 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6001 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6061 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6121 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6181 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6241 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6301 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6361 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6421 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6481 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6541 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6601 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6661 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6721 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6781 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6841 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6901 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6961 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7021 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7081 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7141 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7201 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7261 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7321 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7381 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7441 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7501 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     7561 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7621 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7681 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7741 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7801 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7861 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7921 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattcccg
//
