LOCUS 40924_49577 7963 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast episomal vector with a URA3 marker for construction of lacZ fusions. The MCS is reversed in YEp357R. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7963) AUTHORS Myers AM, Tzagoloff A, Kinney DM, Lusty CJ. TITLE Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. JOURNAL Gene 1986;45:299-310. PUBMED 3026915 REFERENCE 2 (bases 1 to 7963) TITLE Direct Submission REFERENCE 3 (bases 1 to 7963) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; date: "1986"; volume: "45"; pages: "299-310" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..7963 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..57 /label=MCS /note="pUC18/19 multiple cloning site" CDS 64..3111 /codon_start=1 /label=lacZ /note="beta-galactosidase" /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD DSWSPSVSAELQLSAGRYHYQLVWCQK" CDS complement(3312..4112) /codon_start=1 /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(4113..4323) /label=URA3 promoter rep_origin complement(4560..5724) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication" promoter 6006..6110 /label=AmpR promoter CDS 6111..6968 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7142..7730 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgct 61 cccgccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 181 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca 241 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc 301 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc 361 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca 421 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt 481 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt 541 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg 601 atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc 661 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat 721 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg 781 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag 841 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc 901 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg 961 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa 1021 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc 1081 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc 1141 atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc 1201 gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc 1261 ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg 1321 accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc 1381 gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct 1441 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat 1501 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg 1561 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta 1621 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt 1681 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc 1741 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg 1801 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg 1861 gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt 1921 ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat 1981 agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt 2041 gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg 2101 cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc 2161 gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc 2221 agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat 2281 ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca 2341 cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt 2401 gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg 2461 gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg 2521 gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg 2581 aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt 2641 accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc 2701 cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat 2761 cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat 2821 accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat 2881 ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa 2941 ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc 3001 gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa 3061 ttacagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa 3121 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttcgcc 3181 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac 3241 tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat 3301 acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg 3361 taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata 3421 ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg 3481 tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct 3541 cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca 3601 atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca tgacaattct 3661 gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg 3721 ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt 3781 ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg 3841 tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc 3901 tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct 3961 aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag 4021 tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta 4081 gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttttt gttctgtgca 4141 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata 4201 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa 4261 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt 4321 gaaaagctac ttgttaccca tcattgaatt ttgaacatcc gaacctggga gttttccctg 4381 aaacagatag tatatttgaa cctgtataat aatatatagt ctagcgcttt acggaagaca 4441 atgtatgtat ttcggttcct ggagaaacta ttgcatctat tgcataggta atcttgcacg 4501 tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact tcaatagcat atctttgtta 4561 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc 4621 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt 4681 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat 4741 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct 4801 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg 4861 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc 4921 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt 4981 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag 5041 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg 5101 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga 5161 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat 5221 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag 5281 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga 5341 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat 5401 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc 5461 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct 5521 ctgaagttcc tatactttct agctagagaa taggaacttc ggaataggaa cttcaaagcg 5581 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt 5641 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata 5701 gtgcgtgttt atgcttaaat gcgttatggt gcactctcag tacaatctgc tctgatgccg 5761 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 5821 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 5881 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt 5941 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 6001 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 6061 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 6121 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 6181 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 6241 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 6301 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 6361 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 6421 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 6481 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 6541 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 6601 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 6661 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 6721 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 6781 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 6841 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 6901 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 6961 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 7021 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 7081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 7141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 7201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 7261 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 7321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 7381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 7441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 7501 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag 7561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 7621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 7681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 7741 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 7801 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 7861 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 7921 tacgcaaacc gcctctcccc gcgcgttggc cgattcattc ccg //