LOCUS       40924_49577        7963 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp357R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7963)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7963)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             64..3111
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3312..4112)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4113..4323)
                     /label=URA3 promoter
     rep_origin      complement(4560..5724)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6006..6110
                     /label=AmpR promoter
     CDS             6111..6968
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7142..7730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgct
       61 cccgccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      181 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
      241 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc
      301 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc
      361 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca
      421 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt
      481 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt
      541 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg
      601 atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc
      661 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat
      721 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg
      781 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag
      841 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc
      901 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg
      961 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa
     1021 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc
     1081 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc
     1141 atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc
     1201 gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc
     1261 ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg
     1321 accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc
     1381 gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct
     1441 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat
     1501 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg
     1561 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta
     1621 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt
     1681 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc
     1741 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg
     1801 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg
     1861 gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt
     1921 ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat
     1981 agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt
     2041 gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg
     2101 cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc
     2161 gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc
     2221 agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat
     2281 ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca
     2341 cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt
     2401 gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg
     2461 gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg
     2521 gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg
     2581 aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt
     2641 accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc
     2701 cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat
     2761 cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat
     2821 accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat
     2881 ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa
     2941 ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc
     3001 gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa
     3061 ttacagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
     3121 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttcgcc
     3181 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac
     3241 tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat
     3301 acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg
     3361 taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata
     3421 ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg
     3481 tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct
     3541 cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca
     3601 atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca tgacaattct
     3661 gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg
     3721 ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt
     3781 ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg
     3841 tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc
     3901 tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct
     3961 aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag
     4021 tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta
     4081 gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttttt gttctgtgca
     4141 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
     4201 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
     4261 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt
     4321 gaaaagctac ttgttaccca tcattgaatt ttgaacatcc gaacctggga gttttccctg
     4381 aaacagatag tatatttgaa cctgtataat aatatatagt ctagcgcttt acggaagaca
     4441 atgtatgtat ttcggttcct ggagaaacta ttgcatctat tgcataggta atcttgcacg
     4501 tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact tcaatagcat atctttgtta
     4561 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
     4621 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
     4681 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
     4741 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
     4801 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
     4861 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     4921 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     4981 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     5041 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     5101 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     5161 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
     5221 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
     5281 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
     5341 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     5401 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
     5461 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
     5521 ctgaagttcc tatactttct agctagagaa taggaacttc ggaataggaa cttcaaagcg
     5581 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
     5641 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
     5701 gtgcgtgttt atgcttaaat gcgttatggt gcactctcag tacaatctgc tctgatgccg
     5761 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5821 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5881 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     5941 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6001 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6061 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6121 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6181 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6241 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6301 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6361 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6421 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6481 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6541 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6601 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6661 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6721 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6781 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6841 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6901 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6961 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7021 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7261 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7501 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     7561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7741 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7801 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7861 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7921 tacgcaaacc gcctctcccc gcgcgttggc cgattcattc ccg
//
