LOCUS       40924_49572        7972 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp356.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7972)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7972)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             73..3120
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3321..4121)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4122..4332)
                     /label=URA3 promoter
     rep_origin      complement(4569..5733)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6015..6119
                     /label=AmpR promoter
     CDS             6120..6977
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7151..7739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattccca
       61 gcttgcgatc ccgccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      121 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      181 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt
      241 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact
      301 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgta
      361 acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac
      421 tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt
      481 gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag
      541 gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc
      601 ctcgcggtga tggtgctgcg ttggagtgac ggcagttatc tggaagatca ggatatgtgg
      661 cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc
      721 gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa
      781 gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt
      841 gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt
      901 ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc
      961 gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt
     1021 gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg
     1081 ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat
     1141 ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac
     1201 tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac
     1261 cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg
     1321 aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg
     1381 gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc
     1441 cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg
     1501 gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac
     1561 gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg
     1621 ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt
     1681 aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag
     1741 ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac
     1801 ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg
     1861 aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag
     1921 cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg
     1981 ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg
     2041 gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct
     2101 gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg
     2161 aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg
     2221 gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc
     2281 gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc
     2341 tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag
     2401 ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct
     2461 aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg
     2521 cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag
     2581 catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa
     2641 atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc
     2701 ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa
     2761 gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca
     2821 gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa
     2881 ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt
     2941 caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg
     3001 ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta
     3061 tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa
     3121 taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac
     3181 tatttcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag
     3241 ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt
     3301 acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct
     3361 gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt
     3421 ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga
     3481 cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa
     3541 ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg
     3601 ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat
     3661 gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc
     3721 ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat
     3781 tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca
     3841 aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta
     3901 actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt
     3961 ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa
     4021 gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta
     4081 ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct tcgttttttg
     4141 ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc
     4201 gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta
     4261 ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg
     4321 aattgaattg aaaagctact tgttacccat cattgaattt tgaacatccg aacctgggag
     4381 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
     4441 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
     4501 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
     4561 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
     4621 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
     4681 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
     4741 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     4801 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
     4861 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
     4921 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
     4981 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
     5041 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
     5101 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
     5161 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
     5221 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
     5281 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
     5341 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
     5401 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     5461 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     5521 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac
     5581 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
     5641 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
     5701 aacggcatag tgcgtgttta tgcttaaatg cgttatggtg cactctcagt acaatctgct
     5761 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5821 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5881 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5941 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6001 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6061 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7261 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     7561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7681 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7801 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7861 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7921 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattcc cg
//
