LOCUS       40924_49567        7965 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. The MCS is reversed in YEp356R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7965)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7965)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             66..3113
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
                     SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP
                     PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE
                     FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT
                     RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR
                     VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG
                     LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG
                     ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL
                     SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN
                     PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY
                     LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV
                     QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR
                     QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH
                     PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL
                     RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD
                     DSWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(3314..4114)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4115..4325)
                     /label=URA3 promoter
     rep_origin      complement(4562..5726)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6008..6112
                     /label=AmpR promoter
     CDS             6113..6970
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7144..7732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgcg
       61 atcccgccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      121 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      181 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
      241 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
      301 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc
      361 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
      421 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
      481 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc
      541 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg
      601 tgatggtgct gcgttggagt gacggcagtt atctggaaga tcaggatatg tggcggatga
      661 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc
      721 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga
      781 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc
      841 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg
      901 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc
      961 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag
     1021 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg
     1081 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg
     1141 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg
     1201 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg
     1261 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc
     1321 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc
     1381 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg
     1441 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt
     1501 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg
     1561 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc
     1621 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc
     1681 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct
     1741 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt
     1801 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc
     1861 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt
     1921 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc
     1981 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg
     2041 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac
     2101 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga
     2161 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc
     2221 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg
     2281 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt
     2341 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc
     2401 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct
     2461 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
     2521 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
     2581 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
     2641 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
     2701 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
     2761 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
     2821 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt
     2881 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc
     2941 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata
     3001 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg
     3061 aattacagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat
     3121 aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttcg
     3181 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtaata
     3241 actgatataa ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata
     3301 atacagtttt ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc
     3361 tgtaacgttc accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa
     3421 taataatgtc agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg
     3481 cgtctccctt gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat
     3541 ctcttccacc catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg
     3601 caatgtcaac agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt
     3661 ctgctaacat caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac
     3721 cgctaacaat acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta
     3781 ttctgtatac acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc
     3841 tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc
     3901 cctccatgga aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac
     3961 ctaatgcttc aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca
     4021 agtttgtttg cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag
     4081 tagcagcacg ttccttatat gtagctttcg acatgattta tcttcgtttt ttgttctgtg
     4141 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
     4201 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     4261 aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     4321 ttgaaaagct acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc
     4381 tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga
     4441 caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca
     4501 cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt
     4561 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     4621 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     4681 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     4741 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     4801 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     4861 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     4921 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     4981 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     5041 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     5101 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     5161 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     5221 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     5281 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     5341 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     5401 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
     5461 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
     5521 ctctgaagtt cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag
     5581 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     5641 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     5701 tagtgcgtgt ttatgcttaa atgcgttatg gtgcactctc agtacaatct gctctgatgc
     5761 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5821 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5881 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     5941 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6001 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6061 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6121 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6181 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6241 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6301 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6361 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6421 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6481 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6541 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6601 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6661 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6721 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6781 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6841 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6901 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6961 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7021 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7081 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7141 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7201 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7261 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7321 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7381 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7441 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7501 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     7561 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7621 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7681 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7741 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7801 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7861 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7921 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat tcccg
//
