LOCUS       40924_49522       10667 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast episomal vector YEp13, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Rose AB, Broach JR.
  TITLE     Propagation and expression of cloned genes in yeast: 2-microns 
            circle-based vectors
  JOURNAL   Meth. Enzymol. 185, 234-279 (1990)
  PUBMED    2199781
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   Stillman DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-1993) David J. Stillman, Dept. of Cellular, Viral 
            and Molecular Biology, University of Utah Medical Center, Salt Lake 
            City, UT 84132 USA
REFERENCE   3  (bases 1 to 10667)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10667)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Meth. 
            Enzymol. 185, 234-279 (1990)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-1993) David J. Stillman, Dept. of Cellular, Viral and 
            Molecular Biology, University of Utah Medical Center, Salt Lake 
            City, UT 84132 USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..41
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             89..1276
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             1920..2108
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    2213..2353
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2539..3127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3301..4158)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4159..4263)
                     /label=AmpR promoter
     promoter        5435..5842
                     /label=LEU2 promoter
     CDS             5843..6934
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(8988..10330)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
       61 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
      121 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
      181 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
      241 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
      301 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
      361 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      421 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      481 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      541 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      601 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
      661 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
      721 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
      781 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
      841 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
      901 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
      961 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     1021 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     1081 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     1141 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     1201 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     1261 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     1321 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     1381 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt
     1441 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     1501 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     1561 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     1621 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     1681 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     1741 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     1801 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     1861 cgtttcatcg gtatcattac ccccatgaac agaaattccc ccttacacgg aggcatcaag
     1921 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     1981 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     2041 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     2101 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2161 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     2221 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     2281 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2341 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2701 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     2761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2941 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3601 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3901 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4261 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4321 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattcc acggactata
     4381 gactatacta gtatactccg tctactgtac gatacacttc cgctcaggtc cttgtccttt
     4441 aacgaggcct taccactctt ttgttactct attgatccag ctcagcaaag gcagtgtgat
     4501 ctaagattct atcttcgcga tgtagtaaaa ctagctagac cgagaaagag actagaaatg
     4561 caaaaggcac ttctacaatg gctgccatca ttattatccg atgtgacgct gcagaagcag
     4621 aaatacacgc ggtcagtgaa gctattccgc tattgaataa cctcagtcac cttgtgcaag
     4681 aacttaacaa gaaaccaatt attaaaggct tacttactga tagtagatca acgatcagta
     4741 taattaagtc tacaaatgaa gagaaattta gaaacagatt ttttggcaca aaggcaatga
     4801 gacttagaga tgaagtatca ggtaataatt tatacgtata ctacatcgag accaagaaga
     4861 acattgctga tgtgatgaca aaacctcttc cgataaaaac atttaaacta ttaactaaca
     4921 aatggattca ttagatctat tacattatgg gtggtatgtt ggaataaaaa tcaactatca
     4981 tctactaact agtatttacg ttactagtat attatcatat acggtgttag aagatgacgc
     5041 aaatgatgag aaatagtcat ctaaattagt ggaagctgaa acgcaaggat tgataatgta
     5101 ataggatcaa tgaatattaa catataaaat gatgataata atatttatag aattgtgtag
     5161 aattgcagat tcccttttat ggattcctaa atcctcgagg agaacttcta gtatatctac
     5221 atacctaata ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat
     5281 tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt
     5341 tataggataa ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa
     5401 ggcgcctgat tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta
     5461 agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca
     5521 cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag
     5581 aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag
     5641 agccgcggaa ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc
     5701 acaatacttg aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta
     5761 gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat
     5821 atttcaagga tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga
     5881 ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt
     5941 tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc
     6001 tacaggtgtc ccacttccag atgaggcgct ggaagcctcc aagaaggttg atgccgtttt
     6061 gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt
     6121 actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc
     6181 cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt
     6241 tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga
     6301 tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat
     6361 ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct tggataaagc
     6421 taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga
     6481 attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa
     6541 gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc
     6601 cgatgaagcc tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc
     6661 tttgccagac aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga
     6721 tttgccaaag aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa
     6781 attgtcattg aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt
     6841 ggatgcaggt atcagaactg gtgatttagg tggttccaac agtaccacgg aagtcggtga
     6901 tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata
     6961 tttgtacata aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg
     7021 aatatgttca tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga
     7081 gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat
     7141 aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa
     7201 gttaggtgta acagaaaatc atgaaactat gattcctaat ttatatattg gaggattttc
     7261 tctaaaaaaa aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag
     7321 tttaagtcaa taccttcttg aaccatttcc cataatggtg aaagttccct caagaatttt
     7381 actctgtcag aaacggcctt aacgacgtag tcgacctcct cttcagtact aaatctacca
     7441 ataccaaatc tgatggaaga atgggctaat gcatcatcct tacccagcgc atgtaaaaca
     7501 taagaaggtt ctagggaagc agatgtacag gctgaacccg aggataatgc gatatccctt
     7561 agtgccatca ataaagattc tccttccacg taggcgaaag aaacgttaac acaccctgga
     7621 taacgatgat ctggagatcc gttcaacgtg gtatgttcag cggataatag acctttgact
     7681 aatttatcgg atagtctttt gatgtgagct tggtcgttgt caaattcttt cttcatcaat
     7741 ctcgcagctt caccaaatcc cgctaccaat gggggggcca aagtaccaga tctcaatcct
     7801 ctctcttggc caccaccgga tagtaaaggt tctaatctaa ctcttggtct ccttcttaca
     7861 tagatggcac ctattccctt tggaccgtaa atcttgtgag aagaaattga tagtaaatca
     7921 atgttcattt cattgacatc aatgtgaatc ttaccatagg cttgtgcggc gtcagtatga
     7981 aagtagatct tattctttct acaaattgca ccaatttctt taataggttg aatgacaccg
     8041 atttcattat tgacagccat cacagagacg agacaggtat ctggtctaat ggcatcttcc
     8101 aattccttca aatcgataag accttgatcg tccacattta ggaaagtgac ttcaaatccc
     8161 tccttcatca tggcccgtgc ggcttccaag acacacttgt gttccgttct agtggtgatg
     8221 atgtgtttct tagtcttctt ataaaatctt gggacaccct taagaaccat attattagat
     8281 tcggtcgctc ccgaagtgaa tattatttcc ttggggtcgg cattgatcat ctttgctacg
     8341 taagctctag cattttccac agcagtattt gtttcccaac cgtaagagtg agtgttggaa
     8401 tgaggattac cataaagtcc cgtataaaac ttcaacatcg tatccaaaac cctagggtct
     8461 gttggtgtag tggcttgcat gtcaagatat atgggacgag taccaaaacc tgtgttttct
     8521 tgataagcat ggctcattgc agtgctacca gaagctacta cagcatctgg ggtggtaccg
     8581 gatgcactcg cacgggcact agcctgtgcc tttgcagcag cctgaatatc ggtatgcgtt
     8641 tccagagaga agttgtcgtc taacttcacg cctgctgcag tctcaatgat attcgaatac
     8701 gctttgagga gatacagcct aatatccgac aaactgtttt acagatttac gatcgtactt
     8761 gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa acagatagta
     8821 tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat gtatgtattt
     8881 cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc gcatccccgg
     8941 ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg
     9001 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     9061 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
     9121 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     9181 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
     9241 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
     9301 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
     9361 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
     9421 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
     9481 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
     9541 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
     9601 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
     9661 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
     9721 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
     9781 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
     9841 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
     9901 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
     9961 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
    10021 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
    10081 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
    10141 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
    10201 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
    10261 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
    10321 atattggatc atatgcatag taccgagaaa ctagtgcgaa gtagtgatca ggtattgctg
    10381 ttatctgatg agtatacgtt gtcctggcca cggcagaagc acgcttatcg ctccaatttc
    10441 ccacaacatt agtcaactcc gttaggccct tcattgaaag aaatgaggtc atcaaatgtc
    10501 ttccaatgtg agattttggg ccatttttta tagcaaagat tgaataaggc gcatttttct
    10561 tcaaagcttt attgtacgat ctgactaagt tatcttttaa taattggtat tcctgtttat
    10621 tgcttgaaga attgccggtc ctatttactc gttttaggac tggttca
//
