LOCUS       40924_49507        3818 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YDp-U, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Stolz J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2002) Cell Biology and Plant Physiology, 
            Regensburg University, Universitaetsstr. 31, Regensburg D-93040, 
            Germany
REFERENCE   2  (bases 1 to 3818)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3818)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg 
            University, Universitaetsstr. 31, Regensburg D-93040, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(118..918)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(919..1135)
                     /label=URA3 promoter
     primer_bind     complement(1202..1218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1226..1242)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1250..1280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1295..1316)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1604..2192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2366..3223)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3224..3328)
                     /label=AmpR promoter
     primer_bind     3802..3818
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagctgggt aataactgat ataattaaat
       61 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
      121 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
      181 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
      241 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
      301 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
      361 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
      421 cttagtatat tctccagtag ctagggagcc cttgcatgac aattctgcta acatcaaaag
      481 gcctctaggt tcctttgtta cttcttccgc cgcctgcttc aaaccgctaa caatacctgg
      541 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
      601 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
      661 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
      721 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
      781 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
      841 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
      901 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
      961 ggttaagaat actgggcaat ttcatgtttc ttcaacacca catatgcgta tatataccaa
     1021 tctaagtctg tgctccttcc ttcgttcttc cttctgctcg gagattaccg aatcaaaaaa
     1081 atttcaaaga aaccggaatc aaaaaaaaga acaaaaaaaa aaaagatgaa ttgaaaagca
     1141 gcttgctcaa tcaatcaccg gatccgtcga cctgcagcca agctagcttg gcgtaatcat
     1201 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1261 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1321 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2041 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     3361 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     3421 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     3481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3541 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     3601 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     3661 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3721 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     3781 ggttttccca gtcacgacgt tgtaaaacga cggccagt
//
