LOCUS       40924_49502        4314 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YDp-L, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Stolz J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2002) Cell Biology and Plant Physiology, 
            Regensburg University, Universitaetsstr. 31, Regensburg D-93040, 
            Germany
REFERENCE   2  (bases 1 to 4314)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4314)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg 
            University, Universitaetsstr. 31, Regensburg D-93040, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..444
                     /label=LEU2 promoter
     CDS             445..1536
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(1698..1714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1722..1738)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1746..1776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1791..1812)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2100..2688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2862..3719)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3720..3824)
                     /label=AmpR promoter
     primer_bind     4298..4314
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagctaact gtgggaatac tcaggtatcg
       61 taagatgcaa gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa
      121 cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc
      181 agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg
      241 agagcgccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca
      301 tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt
      361 tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat atatatatat
      421 atatttcaag gatataccat tctaatgtct gcccctaaga agatcgtcgt tttgccaggt
      481 gaccacgttg gtcaagaaat cacagccgaa gccattaagg ttcttaaagc tatttctgat
      541 gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat
      601 gctacaggtg ttccacttcc agatgaggcg ctggaagcct ccaagaaggc tgatgccgtt
      661 ttgttaggtg ctgtgggtgg tcctaaatgg ggtaccggta gtgttagacc tgaacaaggt
      721 ttactaaaaa tccgtaaaga acttcaattg tacgccaact taagaccatg taactttgca
      781 tccgactctc ttttagactt atctccaatc aagccacaat ttgctaaagg tactgacttc
      841 gttgttgtca gagaattagt gggaggtatt tactttggta agagaaagga agacgatggt
      901 gatggtgtcg cttgggatag tgaacaatac accgttccag aagtgcaaag aatcacaaga
      961 atggccgctt tcatggccct acaacatgag ccaccattgc ctatttggtc cttggataaa
     1021 gctaatgttt tggcctcttc aagattatgg agaaaaactg tggaggaaac catcaagaac
     1081 gaattcccta cattgaaggt tcaacatcaa ttgattgatt ctgccgccat gatcctagtt
     1141 aagaacccaa cccacctaaa tggtattata atcaccagca acatgtttgg tgatatcatc
     1201 tccgatgaag cctccgttat cccaggttcc ttgggtttgt tgccatctgc gtccttggcc
     1261 tctttgccag acaagaacac cgcatttggt ttgtacgaac catgccacgg ttctgctcca
     1321 gatttgccaa agaataaggt caaccctatc gccactatct tgtctgctgc aatgatgttg
     1381 aaattgtcat tgaacttgcc tgaagaaggt aaggccattg aagatgcagt taaaaaggtt
     1441 ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac cgaagtcggt
     1501 gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct ttttttatga
     1561 tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa attacaaaat
     1621 ggaatatgtt catagggtag gctcaatcaa tcaccggatc cgtcgacctg cagccaagct
     1681 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1741 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1801 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1861 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1921 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1981 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2041 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2101 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2161 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2221 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2281 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2341 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2401 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2461 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2521 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2581 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2641 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2701 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2761 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2821 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2881 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2941 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3001 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3061 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3121 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3181 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3241 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3301 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3361 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3421 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3481 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3541 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3601 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3661 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3721 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3781 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3841 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3901 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     3961 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     4021 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     4081 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     4141 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4201 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     4261 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagt
//
