LOCUS       40924_49497        7877 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YDp-K, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Stolz J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2002) Cell Biology and Plant Physiology, 
            Regensburg University, Universitaetsstr. 31, Regensburg D-93040, 
            Germany
REFERENCE   2  (bases 1 to 7877)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7877)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2002) Cell Biology and Plant Physiology, Regensburg 
            University, Universitaetsstr. 31, Regensburg D-93040, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(783..4958)
                     /codon_start=1
                     /label=LYS2
                     /note="alpha-aminoadipate reductase, required for lysine 
                     biosynthesis"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(4959..5198)
                     /label=LYS2 promoter
     primer_bind     complement(5261..5277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5285..5301)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5309..5339)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5354..5375)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5663..6251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6425..7282)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7283..7387)
                     /label=AmpR promoter
     primer_bind     7861..7877
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccgg ggatccggtg attgattgag caagctcgat ttgtctcaac ctgctttgga
       61 agtggacctt gattccttca aaaagcaaag tattgttagc cgtctaaaca tgagtggcaa
      121 gtggcaatca tcaacgaaaa tcgaagcact tgtggaagaa ctatacaaac tgagaagcaa
      181 caagagaacg attaaatcca ttgtgttttc ccagtttacc agtatgctgg atctggtaga
      241 gtggagattg aaaagagctg gatttcaaac agtgaagctt cagggtagta tgtcaccgac
      301 gcaaagagat gaaaccatca agtatttcat gaacaacatt caatgcgagg ttttcttggt
      361 cagtttaaag gcgggcggtg ttgctttgaa tctttgtgaa gcttcgcaag tattcatttt
      421 agacccatgg tggaacccta gtgttgaatg gcaaagtggt gatagagttc atagaattgg
      481 tcagtatcga cctgtgaaga tcacaaggtt ttgcattgag gatagtatag aagcaagaat
      541 cattgaatta caggaaaaaa aggcaaatat gattcatgct acaataaacc aagatgaagc
      601 tgccattagc agactaacgc cagctgattt acagttctta ttcaataact aatattttat
      661 tctcttatta tatattattc tcggagtttt taagtgacat cacccgaaaa gaagctaagt
      721 ctttctccta attcatattt aattattgta catggacata tcatacgtaa tgctcaacct
      781 taagctgctg cggagcttcc acgagcacca gcacctgaag caactagact tatttgcgct
      841 tgagttagtt ctatacttgg cagtggaagt ttgtcattat gagttggtgg aggtaaaaat
      901 ccaaccttgt ttaaaaatgc aatatatata ccaacctctt ctggagtaac acctattcca
      961 ttagaccaat caacacctgt ccatgcggtg tctttcttta aagatgccac ggcgttccta
     1021 tcgtctagtt ccggagcttt ggtactttca ggtaagttgt ctaagaccat gtgtagcaaa
     1081 ggatacaacg cattttcttc attcctgtca ataacagacg cctccaatga tttcttccat
     1141 ttagaatagc tttcgatttc gacatcgtaa ccataatcgt gtaaagtata caagtagtct
     1201 ttgaataata ttcttgggtg acccgttact tgagcaacgg ccaattcatt ttctttggga
     1261 ggattcaaag acgtagcaac aacaacacga gcaacatgat ctactggaac catattcacg
     1321 gaattttcga tatctggaat cttacctaat tggactgaac ctttcaaaaa tctcaataag
     1381 aaatcatctg tgtttgaaga accattggca gaggcacctg ttacgtaacc tggtctgaca
     1441 atacacccac gtaggcccct ttcacctgca cgtctaatga tgtactcagc agcccatttg
     1501 gactgaccat atccaccagt gagcccgctt gcagagttca ttaaatcgtc tgattctaaa
     1561 atgcctggct tcccttcgct aacaagttta tctgacaaat taaagtagta ttcagtgtca
     1621 agagtagaag tggaggaaac aaagtcaaag aactttggct tgccgacggc ggctaagctc
     1681 ataacattga tagttgaaat aacatttgga tccctcaatt tggcatatgg ataaacccag
     1741 tgaactaacg caccattatg gataattata tcaactgtgt ttgccaaatc catccacttc
     1801 tcatctgaaa gaccaaattg gcttttagat aaatcgccta atacaacttt aatatttgag
     1861 gcaaattttt cgttccaagt accataggtg atacctgcct tttgtaatct tgcaaatgca
     1921 gcttcttcat ccttggccct gacgtgggca aacactttga aactgtagtt ctttggagaa
     1981 cgtcctaaca aatctgcaag gatgtaggag cccagaaatc ctgtgacacc ggtaacaaac
     2041 acattaattg ttgtttttcc ttcggcacta ttaggttcaa caaaatattc tcgagagggg
     2101 tacgaacttg gtagcgtctc aaccaatttc ttggcgtctt ccgcataatt tgcagtgaca
     2161 ttttcgacga cctcaccttg agatgatcca cccgatgatt taattctgtc aatttccgcg
     2221 gcaaaggcct ttatcgttgg atacttgaaa attgtgccca atggtaaatc aacttgcagc
     2281 tttttcttta aggtaaaaat cattttggta gccaagatag aatgaccacc taaatcgaaa
     2341 aacgaatcat ctggtgatac agatgctggc ttggtaggta atatacttaa ccataagtct
     2401 ctaacctcgc gctcaacatt ggtaaactga gagtcgtcag tttcagaaac tgtattttca
     2461 gctaccaaat ttaattgctt gggagttggg aattgaagtt taggcttatc aactttacca
     2521 tttggattca atggtagttt atccataacc acaatcaagg aaggcatagc atagctagcc
     2581 aatcttttct ttaagaaagt cctgatgtcc ttggataaaa gatggtaacc gattaagccc
     2641 ttaactatag ggtcagtttc aacctccttt ggaacatcac tttggaactt agacaagtca
     2701 tctggcttgt caaatcttgg gaccataaat gtgatcaatg ttggctcatt gtcggcattt
     2761 ttgcgaacta aagtaatgtt ttctcttacc aatggatgtt gggaaatgtg cgtatctatt
     2821 tctcctaatt cgattctgaa cccacgaatt ttaacttgat catcagccct accgcaacat
     2881 tcacagtcac cgtttggtag ataacgacct aaatcacccg ttctgtacaa tctatctctt
     2941 ggacctaacc agaattgtct ccaaggttca ccattatcct tatccaaata attccagtga
     3001 tctttttcaa caaaccagtt gttcacaaat ttttctttat tcaattctgg taatcctcta
     3061 taaccttcgg ccaaaccacc tgcacgaaca taaatctcac ctatttcgcc aataccacat
     3121 atttgagtac gatcgttcct gttaacaact agtagctgaa cgttcaacat acctttacca
     3181 gcaggcatga catctttcaa ttttttcaaa aagtttggat cgtcattttt tgatttaact
     3241 tcgaaataag aaactgcacg ctgtgtttca gtggtaccgt acatattaac aatacggcaa
     3301 ttttctgcca aggtttgtaa cctcagacaa tcacgttttg ttaaaatgtc acccacaaag
     3361 aacgcatgat gtaacttagg gaatggtgta gtagcttggg cagtaagtaa ttgacccatg
     3421 gcaggtgtta aatgggtaac tgtgcaacca tacttactca tccattccgc taaacggccc
     3481 ggtgtaccaa tatcatcttg agtagggaca tacaattggg cacctaaaaa taatggtgta
     3541 aacatatctc tttgaattgg atcatgtgca ataccgctca gcattgtgaa tttatcattt
     3601 tctgttaagt tgaacctttt ggacatccaa ttgaaataat aagccaagga aaaatgtcta
     3661 ccaagaacac ccttaggaat accttcggaa ccagatgtga aagatagggt tgggttggaa
     3721 tctggtccaa ctacaacacc tgttctggtg tctttgtagt gatcatatgg agccaaaaca
     3781 tcctcgccat tgtccaattt gccaccttca atggtaccat tttcttgaat agcgatggaa
     3841 ttgattcttg aaacaatctc caattcatca ttgatgtaat cttctactag ttgatccaat
     3901 tgtccagcag ctctaataac aatcaaccca cgtggtttag caacacctaa gtaaatggtt
     3961 tgtctggctg ggggatatgc agggtcgata actgaaaagg ttgcgccggc tttcaagaca
     4021 cccatcacac ataccatcaa atccacaccc ctagaagaat agatcatcac tacatcacct
     4081 cttttgatac ctgttttaat caaataatgg gcaactatgt tagaagtgcg gttgatgtcg
     4141 cgataagtga aagaacggga cttgtcggaa tttagtgttg gagtctccac aacacaggtt
     4201 ctctctggga aggcttcagc attgtcctgg aaaatgtcgt gaatacaccc cacgaaatcg
     4261 caccagccca agttcttagt tggatcaggt aaactatcct tggatgatgc ggtgatcaga
     4321 gagattttag ttatgcaatt ggatggatcg cttagcgcag cagtcaaata ttgagtaaat
     4381 tggtccgcaa caatggttac tctttcattc gaatacagta agctgttata aattaagttc
     4441 aaaacatgcg cattattact ggtatccaaa ttcaaagcaa agtccactaa atgatgcttg
     4501 aactcgtcta atttgaaatc ttggttttcc aaaaaggcca aacggaacaa ctgaggggtc
     4561 ctttccagat cttggcaact ttgaattttt tcagctagct cgtcaaagga aaaattggcc
     4621 tcaatagaat tgagcttgtt caactcattg ttaattgtag aatacagctc attaaatgac
     4681 cacgttggtt gaatattgaa tcttaagatt ttgttattcg caatataaag aacaatatca
     4741 tcgtcaccgg ttactctata tatcaatgca gcccatacac tcaaagcgac agcgtatttg
     4801 ttagaaaaac tatcatgagg cacatcgagc tgaggtagct gtaacgaata tgtagcttgt
     4861 ttcgtataag gttcttgttg tgggcgtaaa aagtcatgtg gtaacactga aagagttgga
     4921 ttatccaact tctctatcca gaccttttcg ttagtcatta gtgagtaact ctgtgatatc
     4981 tctctataat tagcagtttt tcactgaaat tccaggaaag gtaataaact cagatttttt
     5041 tttatactat tggctgcttg ttacttatat atcttgaact tctcccagcg ggtcttcaaa
     5101 tacatttggg cgatgttcat gttcattagg caggtatttc gacattgagt cacacgcgaa
     5161 aaaccgccgg aattttttat gtaattgcaa gtggaattag cttgctcaat caatcaccgg
     5221 atccgtcgac ctgcagccaa gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5281 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5341 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5401 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5461 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5521 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5581 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5641 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5701 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5761 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5821 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5881 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6001 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6061 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6121 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6181 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6241 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6301 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6361 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6421 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6481 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6541 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6601 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6661 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6721 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6781 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6841 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6901 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6961 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7021 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7081 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7141 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7201 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7261 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7321 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7381 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     7441 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     7501 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     7561 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     7621 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     7681 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     7741 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     7801 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     7861 gtaaaacgac ggccagt
//
