LOCUS       40924_49447        7950 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast centromere-containing shuttle vector YCp50.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Rose MD, Novick P, Thomas JH, Botstein D, Fink GR.
  TITLE     A Saccharomyces cerevisiae genomic plasmid bank based on a 
            centromere-containing shuttle vector.
  JOURNAL   Gene 1987;60:237-43.
  PUBMED    3327750
REFERENCE   2  (bases 1 to 7950)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7950)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1987"; volume: "60"; pages: "237-43"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..41
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             89..1276
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        1440..1660
                     /label=URA3 promoter
     CDS             1661..2461
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    complement(3809..5658)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     misc_feature    5800..5940
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6126..6714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6888..7745)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7746..7850)
                     /label=AmpR promoter
ORIGIN
        1 gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
       61 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
      121 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
      181 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
      241 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
      301 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
      361 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      421 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      481 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      541 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      601 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
      661 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
      721 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
      781 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
      841 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
      901 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
      961 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     1021 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     1081 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     1141 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     1201 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     1261 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     1321 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     1381 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatcgg ggggggggtt
     1441 tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt
     1501 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
     1561 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
     1621 actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa
     1681 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
     1741 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
     1801 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
     1861 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
     1921 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
     1981 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
     2041 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
     2101 tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
     2161 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
     2221 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
     2281 tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg
     2341 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
     2401 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
     2461 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
     2521 ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata atgacgaaaa
     2581 aaaaaaaatt ggaaagaaaa cccccccccc gcagcgttgg gtcctggcca cgggtgcgca
     2641 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     2701 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     2761 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     2821 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     2881 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     2941 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     3001 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     3061 ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
     3121 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     3181 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     3241 ttaccgcagg tgggccattc tcatgaagaa tatcttgaat ttattgtcat attactagtt
     3301 ggtgtggaag tccatatatc ggtgatcaat atagtggttg acatgctggc tagtcaacat
     3361 tgagcctttt gatcatgcaa atatattacg gtattttaca atcaaatatc aaacttaact
     3421 attgacttta taacttattt aggtggtaac attcttataa aaaagaaaaa aattactgca
     3481 aaacagtact agcttttaac ttgtatccta ggttatctat gctgtctcac catagagaat
     3541 attacctatt tcagaatgta tgtccatgat tcgccgggta aatacatata atacacaaat
     3601 ctggcttaat aaagtctata atatatctca taaagaagtg ctaaattggc tagtgctata
     3661 tatttttaag aaaatttctt ttgactaagt ccatatcgac tttgtaaaag ttcactttag
     3721 catacatata ttacacgagc cagaaattgt aacttttgcc taaaatcaca aattgcaaaa
     3781 tttaattgct tgcaaaaggt cacatgctta taatcaactt ttttaaaaat ttaaaatact
     3841 tttttatttt ttatttttaa acataaatga aataatttat ttattgttta tgattaccga
     3901 aacataaaac ctgctcaaga aaaagaaact gttttgtcct tggaaaaaaa gcactaccta
     3961 ggagcggcca aaatgccgag gctttcatag cttaaactct ttacagaaaa taggcattat
     4021 agatcagttc gagttttctt attcttcctt ccggttttat cgtcacagtt ttacagtaaa
     4081 taagtatcac ctcttagagt taactatgag ataagcaagt atcatctcat ttcatttacc
     4141 tgaagtcgag taaacagaaa atccaattgt tgatgaacct caatgactta gaactatcta
     4201 tcggcagatc atataaagag gatttaggta cctagaggac tgtacctgga gtatatatat
     4261 atatatatat atattatctc aactatagtc catagaggtt tctttcttga ggccttaaac
     4321 tgctaaagaa tgatattggt ggaatgcaag caccaatctc tcttctttcg taactgttca
     4381 tatacttcaa accaagaatg taacgggcat tgacccatcc aaaaccttca gtagctgccc
     4441 ctttaaagtc agcaccttga ttaccgtatt ctgcttcaac acgatgagga tctgttcctc
     4501 ttgtgacatc atatttttca accacaatac cattataatc gacaaaagcc tttgtcatca
     4561 tgaaaagcca tctataagct agcctattcg ttacagttaa ataaccataa gaacggaggc
     4621 cttcccaagc aagaatttga tggggtgccc aaccaaatgg atagtcccat tgtctaattg
     4681 gtctcgaaat agaaattggg cctcgagaac gctccgtaca tgcagctaaa cctccaagca
     4741 tctctaactt gggtagtgct ttctccacca ttttctgtgc ttgctccttc gtggcaagtc
     4801 cagcccataa tgcccagaat gtagttgcgg attcgtatga cgttctgtgc ttgatttttg
     4861 tgttgtagtc aaagaaaaac cccgactcgt catcccacat atatttggta attgatgagg
     4921 caacgctaat tatcaacata tagattgtta tctatctgca tgaacacgaa atctttactt
     4981 gacgacttga ggctgatggt gtttatgcaa agaaaccact gtgtttaata tgtgtcactg
     5041 tttgatatta ctgtcagcgt agaagataat agtaaaagcg gttaataagt gtatttgaga
     5101 taagtgtgat aaagttttta cagcgaaaag acgataaata caagaaaatg attacgagga
     5161 tacggagaga ggtatgtaca tgtgtattta tatactaagc tgccggcggt tgtttgcaag
     5221 accgagaaaa ggctagcaag aatcgggtca ttgtagcgta tgcgcctgtg aacattctct
     5281 tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc aaccccctgc gatgtatatt
     5341 ttcctgtaca atcaatcaaa aagccaaatg atttagcatt atctttacat cttgttattt
     5401 tacagatttt atgtttagat cttttatgct tgcttttcaa aaggcctgca ggcaagtgca
     5461 caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat ttttgacgaa
     5521 atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc ttacatcaac
     5581 accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca gtccaccagc
     5641 taacataaaa tgtaagctct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     5701 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     5761 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     5821 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     5881 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     5941 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6001 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6541 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7201 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7261 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7321 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7381 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7441 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7501 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7561 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7621 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7681 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7741 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7801 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7861 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7921 aggcgtatca cgaggccctt tcgtcttcaa
//
