LOCUS 40924_49447 7950 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast centromere-containing shuttle vector YCp50. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7950) AUTHORS Rose MD, Novick P, Thomas JH, Botstein D, Fink GR. TITLE A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. JOURNAL Gene 1987;60:237-43. PUBMED 3327750 REFERENCE 2 (bases 1 to 7950) TITLE Direct Submission REFERENCE 3 (bases 1 to 7950) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; date: "1987"; volume: "60"; pages: "237-43" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..7950 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 13..41 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS 89..1276 /codon_start=1 /label=TcR /note="tetracycline efflux protein" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" promoter 1440..1660 /label=URA3 promoter CDS 1661..2461 /codon_start=1 /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" misc_feature complement(3809..5658) /label=CEN/ARS /note="S. cerevisiae CEN4 centromere fused to the autonomously replicating sequence ARS1/ARS416" misc_feature 5800..5940 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(6126..6714) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6888..7745) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7746..7850) /label=AmpR promoter ORIGIN 1 gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt 61 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct 121 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct 181 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct 241 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg 301 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc 361 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc 421 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg 481 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg 541 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg 601 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc 661 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 721 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 781 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc 841 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac 901 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta 961 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc 1021 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga 1081 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg 1141 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg 1201 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag 1261 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca 1321 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag 1381 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatcgg ggggggggtt 1441 tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt 1501 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt 1561 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca 1621 actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa 1681 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga 1741 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt 1801 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga 1861 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt 1921 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc 1981 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc 2041 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct 2101 tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg 2161 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga 2221 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga 2281 tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg 2341 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga 2401 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa 2461 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat 2521 ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata atgacgaaaa 2581 aaaaaaaatt ggaaagaaaa cccccccccc gcagcgttgg gtcctggcca cgggtgcgca 2641 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc 2701 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga 2761 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga 2821 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct 2881 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct 2941 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat 3001 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc 3061 ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc 3121 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga 3181 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct 3241 ttaccgcagg tgggccattc tcatgaagaa tatcttgaat ttattgtcat attactagtt 3301 ggtgtggaag tccatatatc ggtgatcaat atagtggttg acatgctggc tagtcaacat 3361 tgagcctttt gatcatgcaa atatattacg gtattttaca atcaaatatc aaacttaact 3421 attgacttta taacttattt aggtggtaac attcttataa aaaagaaaaa aattactgca 3481 aaacagtact agcttttaac ttgtatccta ggttatctat gctgtctcac catagagaat 3541 attacctatt tcagaatgta tgtccatgat tcgccgggta aatacatata atacacaaat 3601 ctggcttaat aaagtctata atatatctca taaagaagtg ctaaattggc tagtgctata 3661 tatttttaag aaaatttctt ttgactaagt ccatatcgac tttgtaaaag ttcactttag 3721 catacatata ttacacgagc cagaaattgt aacttttgcc taaaatcaca aattgcaaaa 3781 tttaattgct tgcaaaaggt cacatgctta taatcaactt ttttaaaaat ttaaaatact 3841 tttttatttt ttatttttaa acataaatga aataatttat ttattgttta tgattaccga 3901 aacataaaac ctgctcaaga aaaagaaact gttttgtcct tggaaaaaaa gcactaccta 3961 ggagcggcca aaatgccgag gctttcatag cttaaactct ttacagaaaa taggcattat 4021 agatcagttc gagttttctt attcttcctt ccggttttat cgtcacagtt ttacagtaaa 4081 taagtatcac ctcttagagt taactatgag ataagcaagt atcatctcat ttcatttacc 4141 tgaagtcgag taaacagaaa atccaattgt tgatgaacct caatgactta gaactatcta 4201 tcggcagatc atataaagag gatttaggta cctagaggac tgtacctgga gtatatatat 4261 atatatatat atattatctc aactatagtc catagaggtt tctttcttga ggccttaaac 4321 tgctaaagaa tgatattggt ggaatgcaag caccaatctc tcttctttcg taactgttca 4381 tatacttcaa accaagaatg taacgggcat tgacccatcc aaaaccttca gtagctgccc 4441 ctttaaagtc agcaccttga ttaccgtatt ctgcttcaac acgatgagga tctgttcctc 4501 ttgtgacatc atatttttca accacaatac cattataatc gacaaaagcc tttgtcatca 4561 tgaaaagcca tctataagct agcctattcg ttacagttaa ataaccataa gaacggaggc 4621 cttcccaagc aagaatttga tggggtgccc aaccaaatgg atagtcccat tgtctaattg 4681 gtctcgaaat agaaattggg cctcgagaac gctccgtaca tgcagctaaa cctccaagca 4741 tctctaactt gggtagtgct ttctccacca ttttctgtgc ttgctccttc gtggcaagtc 4801 cagcccataa tgcccagaat gtagttgcgg attcgtatga cgttctgtgc ttgatttttg 4861 tgttgtagtc aaagaaaaac cccgactcgt catcccacat atatttggta attgatgagg 4921 caacgctaat tatcaacata tagattgtta tctatctgca tgaacacgaa atctttactt 4981 gacgacttga ggctgatggt gtttatgcaa agaaaccact gtgtttaata tgtgtcactg 5041 tttgatatta ctgtcagcgt agaagataat agtaaaagcg gttaataagt gtatttgaga 5101 taagtgtgat aaagttttta cagcgaaaag acgataaata caagaaaatg attacgagga 5161 tacggagaga ggtatgtaca tgtgtattta tatactaagc tgccggcggt tgtttgcaag 5221 accgagaaaa ggctagcaag aatcgggtca ttgtagcgta tgcgcctgtg aacattctct 5281 tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc aaccccctgc gatgtatatt 5341 ttcctgtaca atcaatcaaa aagccaaatg atttagcatt atctttacat cttgttattt 5401 tacagatttt atgtttagat cttttatgct tgcttttcaa aaggcctgca ggcaagtgca 5461 caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat ttttgacgaa 5521 atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc ttacatcaac 5581 accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca gtccaccagc 5641 taacataaaa tgtaagctct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 5701 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 5761 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 5821 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 5881 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 5941 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 6001 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 6061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 6121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 6181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 6241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 6301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 6361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 6421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 6481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 6541 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 6601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 6721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 6781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 6841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 6901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 6961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 7021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 7081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 7141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 7201 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 7261 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 7321 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 7381 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 7441 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 7501 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 7561 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7621 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7681 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 7741 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7801 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7861 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 7921 aggcgtatca cgaggccctt tcgtcttcaa //