LOCUS       40924_49437       12837 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary expression vector YC3.6-N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12837)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     FRET-based genetically encoded sensors allow high-resolution live 
            cell imaging of Ca(2) dynamics
  JOURNAL   Plant J. 69 (1), 181-192 (2012)
  PUBMED    21910770
REFERENCE   2  (bases 1 to 12837)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2011) Department of Developmental Biology, Centre 
            for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 12837)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12837)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "1"; pages: "181-192"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2011) Department of Developmental Biology, Centre for 
            Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 
            69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    303..324
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        339..369
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    377..393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     401..417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      444..1089
                     /label=AtUBQ10
                     /note="AtUBQ10"
                     /regulatory_class="promoter"
     CDS             1108..1791
                     /label=CFP
                     /note="cyan variant of GFP"
     CDS             2248..2325
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             2548..3066
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_feature    3075..3138
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      3140..3781
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3794..3810)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4392..4644)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4843..5634)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5664..6044)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    complement(6416..6440)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6968..7756
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      8003..8591
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8777..8917)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9261..9455)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9524..10588)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11025..11651)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      241 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      301 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      361 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      421 gattacgaat tcgagctcgg tacccgacga gtcagtaata aacggcgtca aagtggttgc
      481 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
      541 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
      601 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
      661 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
      721 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
      781 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
      841 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
      901 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
      961 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt
     1021 tagtttctag tttgtgcgat cgaatttgta gattaatctg agtttttctg attaacagct
     1081 cgagtgcggg atcctgcggc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     1141 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc
     1201 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1261 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
     1321 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1381 ggctacgtcc aggagcgtac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1441 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1501 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc
     1561 tatatcaccg ccgacaagca gaagaacggc atcaaggccc acttcaagat ccgccacaac
     1621 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1681 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc ccgcatgcat
     1801 gaccaactga cagaagagca gattgcagag ttcaaagaag ccttctcatt attcgacaag
     1861 gatggggacg gcaccatcac cacaaaggaa cttggcaccg ttatgaggtc gcttggacaa
     1921 aacccaacgg aagcagaatt gcaggatatg atcaatgaag tcgatgctga tggcaatgga
     1981 acgatttact ttcctgaatt tcttactatg atggctagaa aaatgaagga cacagacagc
     2041 gaagaggaaa tccgagaagc attccgtgtt tttgacaagg atgggaacgg ctacatcagc
     2101 gctgctcagt tacgtcacgt catgacaaac ctcggggaga agttaacaga tgaagaagtt
     2161 gatgaaatga taagggaagc agatatcgat ggtgatggcc aagtaaacta tgaagagttt
     2221 gtacaaatga tgacagcaaa gggggggaag aggcgctgga agaaaaactt cattgccgtc
     2281 agcgctgcca accggttcaa gaagatctcc agctccgggg cactggagct catggacggc
     2341 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2401 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     2461 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2521 gagctgtaca agggtggcag cggtggcatg gtgagcaagg gcgaggagct gttcaccggg
     2581 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2641 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     2701 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
     2761 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2821 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2881 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2941 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3001 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3061 atcgagttgt ctagagggcc cgggacgtcc gcggagatct acgcgtgtcg actcgagata
     3121 tccaactagt tggctgcaga gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag
     3181 ttcaatgcat cagtttcatt gcgcacacac cagaatccta ctgagttcga gtattatggc
     3241 attgggaaac atgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat
     3301 tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat
     3361 ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt
     3421 gaatttgaaa atataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg
     3481 tggcctctaa tgaccgaagt taatatgagg agtaaaacac ttgtagttgt accattatgc
     3541 ttattcacta ggcaacaaat atattttcag acctagaaaa gctgcaaatg ttactgaata
     3601 caagtatgtc ctcttgtgtt ttagacattt atgaactttc ctttatgtaa ttttccagaa
     3661 tccttgtcag attctaatca ttgctttata attatagtta tactcatgga tttgtagttg
     3721 agtatgaaaa tattttttaa tgcattttat gacttgccaa ttgattgaca acatgcatca
     3781 atcgaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3841 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3901 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct
     3961 tgccaatcga ggccgaccaa ccgcaagcgt tgtcagtgtt gcaaagcgct ctgtgtgggc
     4021 ctactttaat tgcttccagt gttaaattgg cgaaaggcaa taatatcgca aaatattgtg
     4081 ttgtaaaatg taattatgtt ttaatttcat ggaaatgttt gagcataatt tttattaatg
     4141 tactaaatta ctgttttgtt aaatgcaatt ttgctttctc gggattttaa tatcaaaatc
     4201 tatttagaaa tacacaatat tttgttgcag gcttgctgga gaatcgatct gctatcataa
     4261 aaattacaaa aaaattttat ttgcctcaat tattttagga ttggtattaa ggacgcttaa
     4321 attatttgtc gggtcactac gcatcattgt gattgagaag atcagcgata cgaaatattc
     4381 gtagtactat cgataattta tttgaaaatt cataagaaaa gcaaacgtta catgaattga
     4441 tgaaacaata caaagacaga taaagccacg cacatttagg atattggccg agattactga
     4501 atattgagta agatcacgga atttctgaca ggagcatgtc ttcaattcag cccaaatggc
     4561 agttgaaata ctcaaaccgc cccatatgca ggagcggatc attcattgtt tgtttggttg
     4621 cctttgccaa catgggagtc caaggtttca gggaagctgg aattccgggg tgggcgaaga
     4681 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     4741 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     4801 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     4861 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     4921 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     4981 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5041 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     5101 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5161 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5221 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5281 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     5341 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     5401 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctggagttc
     5461 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     5521 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     5581 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     5641 cgatccgggg aattcgctag agtcgatttg gtgtatcgag attggttatg aaattcagat
     5701 gctagtgtaa tgtattggta atttgggaag atataatagg aagcaaggct atttatccat
     5761 ttctgaaaag gcgaaatggc gtcaccgcga gcgtcacgcg cattccgttc ttgctgtaaa
     5821 gcgttgtttg gtacactttt gactagcgag gcttggcgtg tcagcgtatc tattcaaaag
     5881 tcgttaatgg ctgcggatca agaaaaagtt ggaatagaaa cagaataccc gcgaaattca
     5941 ggcccggttg ccatgtccta cacgccgaaa taaacgacca aattagtaga aaaataaaaa
     6001 ctgactcgga tacttacgtc acgtcttgcg cactgatttg aaaaatctca atataaacaa
     6061 agacggccac aagaaaaaac caaaacaccg atattcatta atcttatcta gtttctcaaa
     6121 aaaattcata tcttccacac gtgaaaatgc caatttctca gacctacctc ggctctgcga
     6181 aggcccccgc tggtatcaaa agtttttatt tcatccgaca tggcgcgacc gacctcaacg
     6241 agaaggaaat tgtcgtgaac ggtgagaagc tctggggcgt gcaaggttcc ggaacgaaca
     6301 tcggtctcaa tgcaaaaggg gaacgccagg ctctgttggc ccctcgaaat tcggcgttaa
     6361 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     6421 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     6481 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     6541 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     6601 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     6661 cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctc gttcggttcg
     6721 taagctgtaa tgcaagtagc gtaactgccg tcacgcaact ggtccagaac cttgaccgaa
     6781 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt cttgttatga catgtttttt
     6841 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     6901 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     6961 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     7021 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     7081 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     7141 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     7201 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     7261 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     7321 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     7381 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     7441 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     7501 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     7561 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     7621 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     7681 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     7741 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgccg
     7801 gcgttaactc aagcgattag atgcactaag cacataattg ctcacagcca aactatcagg
     7861 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     7921 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7981 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8041 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8101 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     8161 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8221 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8281 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8341 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8401 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8461 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8521 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8581 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8641 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8701 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8761 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8821 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     8881 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     8941 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     9001 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     9061 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     9121 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     9181 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     9241 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     9301 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     9361 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     9421 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     9481 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     9541 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     9601 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     9661 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     9721 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     9781 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     9841 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     9901 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     9961 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
    10021 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
    10081 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
    10141 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
    10201 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    10261 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    10321 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    10381 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    10441 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    10501 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    10561 tttgtaccgg gccggatggt ttgcgaccgt cacgccgatt cctcgggctt gggggttcca
    10621 gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct
    10681 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
    10741 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
    10801 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
    10861 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
    10921 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
    10981 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
    11041 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
    11101 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
    11161 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
    11221 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
    11281 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
    11341 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
    11401 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
    11461 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
    11521 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
    11581 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
    11641 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
    11701 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
    11761 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
    11821 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
    11881 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
    11941 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
    12001 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
    12061 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
    12121 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    12181 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    12241 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    12301 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    12361 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    12421 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    12481 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    12541 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    12601 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    12661 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    12721 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    12781 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtact
//
