LOCUS       40924_49432       12830 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary expression vector YC3.6-C, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12830)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     FRET-based genetically encoded sensors allow high-resolution live 
            cell imaging of Ca(2) dynamics
  JOURNAL   Plant J. 69 (1), 181-192 (2012)
  PUBMED    21910770
REFERENCE   2  (bases 1 to 12830)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2011) Department of Developmental Biology, Centre 
            for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 12830)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "1"; pages: "181-192"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2011) Department of Developmental Biology, Centre for 
            Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 
            69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    303..324
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        339..369
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    377..393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     401..417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      445..1089
                     /label=AtUBQ10
                     /note="AtUBQ10"
                     /regulatory_class="promoter"
     misc_feature    1090..1155
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             1156..1839
                     /label=CFP
                     /note="cyan variant of GFP"
     CDS             2296..2373
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             2596..3114
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     terminator      3133..3774
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3787..3803)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4385..4637)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4836..5627)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5657..6037)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    complement(6409..6433)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6961..7749
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      7996..8584
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8770..8910)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9254..9448)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9517..10581)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11018..11644)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      241 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      301 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      361 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      421 gattacgaat tcgagctcgg tacccgacga gtcagtaata aacggcgtca aagtggttgc
      481 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
      541 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
      601 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
      661 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
      721 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
      781 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
      841 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
      901 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
      961 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt
     1021 tagtttctag tttgtgcgat cgaatttgta gattaatctg agtttttctg attaacagct
     1081 cgagtgcggg atcctctaga gggcccggga cgtccgcgga gatctacgcg tgtcgactcg
     1141 agtgcggccg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1201 ctggtcgagc tggacggcga cgtaaacggc cacaggttca gcgtgtccgg cgagggcgag
     1261 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1321 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac
     1381 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1441 gagcgtacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1501 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1561 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc
     1621 gacaagcaga agaacggcat caaggcccac ttcaagatcc gccacaacat cgaggacggc
     1681 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1741 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     1801 cgcgatcaca tggtcctgct ggagttcgtg accgccgccc gcatgcatga ccaactgaca
     1861 gaagagcaga ttgcagagtt caaagaagcc ttctcattat tcgacaagga tggggacggc
     1921 accatcacca caaaggaact tggcaccgtt atgaggtcgc ttggacaaaa cccaacggaa
     1981 gcagaattgc aggatatgat caatgaagtc gatgctgatg gcaatggaac gatttacttt
     2041 cctgaatttc ttactatgat ggctagaaaa atgaaggaca cagacagcga agaggaaatc
     2101 cgagaagcat tccgtgtttt tgacaaggat gggaacggct acatcagcgc tgctcagtta
     2161 cgtcacgtca tgacaaacct cggggagaag ttaacagatg aagaagttga tgaaatgata
     2221 agggaagcag atatcgatgg tgatggccaa gtaaactatg aagagtttgt acaaatgatg
     2281 acagcaaagg gggggaagag gcgctggaag aaaaacttca ttgccgtcag cgctgccaac
     2341 cggttcaaga agatctccag ctccggggca ctggagctca tggacggcgg cgtgcagctc
     2401 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2461 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2521 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2581 ggtggcagcg gtggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2641 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2701 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     2761 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     2821 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2881 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2941 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3001 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     3061 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgagtaaact
     3121 agttggctgc agagctttcg ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg
     3181 catcagtttc attgcgcaca caccagaatc ctactgagtt cgagtattat ggcattggga
     3241 aacatgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt
     3301 tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt
     3361 ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg
     3421 aaaatataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc
     3481 taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta tgcttattca
     3541 ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga atacaagtat
     3601 gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca gaatccttgt
     3661 cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag ttgagtatga
     3721 aaatattttt taatgcattt tatgacttgc caattgattg acaacatgca tcaatcgaag
     3781 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3841 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3901 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgccaat
     3961 cgaggccgac caaccgcaag cgttgtcagt gttgcaaagc gctctgtgtg ggcctacttt
     4021 aattgcttcc agtgttaaat tggcgaaagg caataatatc gcaaaatatt gtgttgtaaa
     4081 atgtaattat gttttaattt catggaaatg tttgagcata atttttatta atgtactaaa
     4141 ttactgtttt gttaaatgca attttgcttt ctcgggattt taatatcaaa atctatttag
     4201 aaatacacaa tattttgttg caggcttgct ggagaatcga tctgctatca taaaaattac
     4261 aaaaaaattt tatttgcctc aattatttta ggattggtat taaggacgct taaattattt
     4321 gtcgggtcac tacgcatcat tgtgattgag aagatcagcg atacgaaata ttcgtagtac
     4381 tatcgataat ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca
     4441 atacaaagac agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga
     4501 gtaagatcac ggaatttctg acaggagcat gtcttcaatt cagcccaaat ggcagttgaa
     4561 atactcaaac cgccccatat gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc
     4621 caacatggga gtccaaggtt tcagggaagc tggaattccg gggtgggcga agaactccag
     4681 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     4741 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     4801 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     4861 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4921 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     4981 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     5041 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
     5101 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     5161 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     5221 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     5281 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     5341 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     5401 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag ttcattcagg
     5461 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     5521 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     5581 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatccg
     5641 gggaattcgc tagagtcgat ttggtgtatc gagattggtt atgaaattca gatgctagtg
     5701 taatgtattg gtaatttggg aagatataat aggaagcaag gctatttatc catttctgaa
     5761 aaggcgaaat ggcgtcaccg cgagcgtcac gcgcattccg ttcttgctgt aaagcgttgt
     5821 ttggtacact tttgactagc gaggcttggc gtgtcagcgt atctattcaa aagtcgttaa
     5881 tggctgcgga tcaagaaaaa gttggaatag aaacagaata cccgcgaaat tcaggcccgg
     5941 ttgccatgtc ctacacgccg aaataaacga ccaaattagt agaaaaataa aaactgactc
     6001 ggatacttac gtcacgtctt gcgcactgat ttgaaaaatc tcaatataaa caaagacggc
     6061 cacaagaaaa aaccaaaaca ccgatattca ttaatcttat ctagtttctc aaaaaaattc
     6121 atatcttcca cacgtgaaaa tgccaatttc tcagacctac ctcggctctg cgaaggcccc
     6181 cgctggtatc aaaagttttt atttcatccg acatggcgcg accgacctca acgagaagga
     6241 aattgtcgtg aacggtgaga agctctgggg cgtgcaaggt tccggaacga acatcggtct
     6301 caatgcaaaa ggggaacgcc aggctctgtt ggcccctcga aattcggcgt taattcagta
     6361 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     6421 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     6481 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     6541 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     6601 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     6661 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctcgttcggt tcgtaagctg
     6721 taatgcaagt agcgtaactg ccgtcacgca actggtccag aaccttgacc gaacgcagcg
     6781 gtggtaacgg cgcagtggcg gttttcatgg cttcttgtta tgacatgttt ttttggggta
     6841 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     6901 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     6961 atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     7021 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     7081 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     7141 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     7201 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     7261 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     7321 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     7381 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     7441 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     7501 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     7561 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     7621 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     7681 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     7741 gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg ccggcgttaa
     7801 ctcaagcgat tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     7861 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     7921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8101 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     8161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8761 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     8821 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     8881 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     8941 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     9001 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     9061 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     9121 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     9181 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     9241 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     9301 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     9361 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     9421 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     9481 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     9541 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     9601 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     9661 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     9721 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     9781 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     9841 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     9901 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     9961 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
    10021 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
    10081 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
    10141 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
    10201 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
    10261 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
    10321 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
    10381 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
    10441 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
    10501 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
    10561 cgggccggat ggtttgcgac cgtcacgccg attcctcggg cttgggggtt ccagtgccat
    10621 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
    10681 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
    10741 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
    10801 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
    10861 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    10921 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    10981 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    11041 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    11101 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    11161 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    11221 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    11281 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    11341 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    11401 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    11461 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    11521 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    11581 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    11641 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    11701 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    11761 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    11821 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    11881 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    11941 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    12001 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    12061 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    12121 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    12181 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    12241 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    12301 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    12361 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    12421 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    12481 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    12541 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    12601 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    12661 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    12721 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    12781 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
//
