LOCUS 40924_49417 8842 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector XN22_GW, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8842) AUTHORS Lalonde S, Sero A, Pratelli R, Pilot G, Chen J, Sardi MI, Parsa SA, Kim DY, Acharya BR, Stein EV, Hu HC, Villiers F, Takeda K, Yang Y, Han YS, Schwacke R, Chiang W, Kato N, Loque D, Assmann SM, Kwak JM, Schroeder JI, Rhee SY, Frommer WB. TITLE A Membrane Protein/Signaling Protein Interaction Network for Arabidopsis Version AMPv2 JOURNAL Front Physiol 1, 24 (2010) PUBMED 21423366 REFERENCE 2 (bases 1 to 8842) AUTHORS Lalonde S, Frommer WB. TITLE Direct Submission JOURNAL Submitted (23-NOV-2010) Plant Biology, Carnegie Institution, 260 Panama St, Stanford, CA 94305, USA REFERENCE 3 (bases 1 to 8842) TITLE Direct Submission REFERENCE 4 (bases 1 to 8842) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Front Physiol 1, 24 (2010)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (23-NOV-2010) Plant Biology, Carnegie Institution, 260 Panama St, Stanford, CA 94305, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8842 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(2..1344) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication" promoter 1603..1883 /label=TRP1 promoter CDS 1884..2555 /label=TRP1 /note="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" rep_origin complement(2661..3116) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 3261..3277 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3302..4006 /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" protein_bind 4016..4140 /label=attR1 /note="recombination site for the Gateway(R) LR reaction" CDS complement(4572..4874) /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" CDS complement(5222..5878) /label=CmR /note="chloramphenicol acetyltransferase" promoter complement(5879..5981) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" protein_bind complement(6159..6283) /label=attR2 /note="recombination site for the Gateway(R) LR reaction" misc_feature 6285..6314 /label=linker /note="linker" misc_feature 6315..6425 /note="NubG; N-terminal moiety of ubiquitin (ubi4)" misc_difference 6351..6353 /label=mutation Ile to Gly /note="mutation Ile to Gly" CDS 6429..6455 /label=HA /note="HA (human influenza hemagglutinin) epitope tag" misc_feature 6456..6458 /label=stop codon /note="stop codon" terminator 6480..6667 /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(6691..6707) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(6715..6731) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6739..6769) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(6784..6805) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7093..7681) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7855..8712) /label=AmpR /note="beta-lactamase" promoter complement(8713..8817) /label=AmpR promoter ORIGIN 1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3301 tatccttttg ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc 3361 atacatcggg attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg 3421 agatatacaa tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat 3481 tacactgcct cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag 3541 ccatcatcat atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat 3601 aggcgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 3661 atatccgcaa tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac 3721 agcaccaaca gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact 3781 ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt 3841 ccagttactt gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga 3901 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 3961 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactcata cgtgaacaag 4021 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta 4081 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg 4141 aatcaactac ttagatggta ttagtgacct gtagtcgacc gacagccttc caaatgttct 4201 tcgggtgatg ctgccaactt agtcgaccga cagccttcca aatgttcttc tcaaacggaa 4261 tcgtcgtatc cagcctactc gctattgtcc tcaatgccgt attaaatcat aaaaagaaat 4321 aagaaaaaga ggtgcgagcc tcttttttgt gtgacaaaat aaaaacatct acctattcat 4381 atacgctagt gtcatagtcc tgaaaatcat ctgcatcaag aacaatttca caactcttat 4441 acttttctct tacaagtcgt tcggcttcat ctggattttc agcctctata cttactaaac 4501 gtgataaagt ttctgtaatt tctactgtat cgacctgcag actggctgtg tataagggag 4561 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg 4621 tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg 4681 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact 4741 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca 4801 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa 4861 accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat 4921 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag 4981 acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga catcatatat 5041 gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca 5101 tacctctttt tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat 5161 cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca cgcggcgttt 5221 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat 5281 ggaagccatc acaracggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 5341 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 5401 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 5461 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat 5521 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt 5581 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac 5641 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa 5701 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat 5761 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt 5821 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt 5881 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta 5941 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa 6001 agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt 6061 gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttag 6121 tcgactacag gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg 6181 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta 6241 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttggtggt ggcggatccg 6301 gtggaggtgg atcaatgcag attttcgtca agactttgac cggtaaaacc ggaacattgg 6361 aagttgaatc ttccgatacc atcgacaacg ttaagtcgaa aattcaagac aaggaaggaa 6421 tccctggtta cccatacgat gttcctgact atgcttagct gcagccaagc taattccggg 6481 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat 6541 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt 6601 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct 6661 ctaccggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 6721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6781 ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6841 aaacctgtcg tgccagctgg attaatgaat cggccaacgc gcggggagag gcggtttgcg 6901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7501 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 7921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 7981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8161 cattgctrca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8461 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 8641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 8701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8821 atttccccga aaagtgccac ct //