LOCUS       62056_23425        5539 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        600..662
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             691..2340
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          2502..2567
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2697..2717
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    3142..3276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3318..3334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3342..3358)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3366..3396)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3411..3432)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3720..4308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4482..5339)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5340..5444)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gttaagatca aagggggtaa
      421 gagcaaaggg ggtaaaatca aagggggccc cctttgatct tacccccttt gatcttaccc
      481 cctttgatct tactgcatgc ctgcaggtcg actctagagg atccggcccc gcccagcgtc
      541 ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact
      601 tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt
      661 aacagcgtca acagcgtgcc gcagatctcc atggaagacg ccaaaaacat aaagaaaggc
      721 ccggcgccat tctatcctct agaggatgga accgctggag agcaactgca taaggctatg
      781 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac
      841 atcacgtacg cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
      901 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
      961 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     1021 gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa
     1081 aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt
     1141 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     1201 catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa
     1261 acaattgcac tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt
     1321 ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa
     1381 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     1441 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     1501 gagctgtttt tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc
     1561 ctattttcat tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     1621 gaaattgctt ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc
     1681 ttccatcttc cagggatacg acaaggatat gggctcactg agactacatc agctattctg
     1741 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     1801 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta
     1861 tgtgtcagag gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     1921 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     1981 cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc
     2041 cccgctgaat tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca
     2101 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     2161 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     2221 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     2281 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg
     2341 taaaatgtaa ctgtattcag cgatgacgaa attcttagct attgtaatga attcgagctc
     2401 caccgcggtg gcggccgctc tagaggatct ttgtgaagga accttacttc tgtggtgtga
     2461 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     2521 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     2581 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     2641 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     2701 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     2761 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     2821 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     2881 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     2941 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     3001 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     3061 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3121 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3241 tccaaactca tcaatgtatc ttatcatgtc tggatctcga gggggggccc ggtaccgagc
     3301 tcgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3361 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3421 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3481 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3541 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3661 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3721 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3781 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3901 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3961 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4021 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4081 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4141 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4201 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5461 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5521 atcacgaggc cctttcgtc
//
