LOCUS       40924_48952        6837 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector Tmp-pSTB205, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Martinez R.
  TITLE     Cloning challenging DNA sequences: a different approach with linear 
            vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Martinez R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2017) Plant Developmental Biology, Max Planck 
            Institute For Plant Breeding Research, Carl-von-Linne Weg 10, 
            Cologne, North Rhine-Westphalia 50829, Germany
REFERENCE   3  (bases 1 to 6837)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6837)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUL-2017) Plant Developmental Biology, Max Planck Institute For 
            Plant Breeding Research, Carl-von-Linne Weg 10, Cologne, North 
            Rhine-Westphalia 50829, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(192..278)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    331..430
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(831..1133)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1478..2134)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2135..2237)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2383..2482)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2485..3201
                     /codon_start=1
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5235..6041
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      6187..6775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct tcgtcgaccg acagccttcc aaatgttctt cgggtgatgc tgccaactta
      481 gtcgaccgac agccttccaa atgttcttct caaacggaat cgtcgtatcc agcctactcg
      541 ctattgtcct caatgccgta ttaaatcata aaaagaaata agaaaaagag gtgcgagcct
      601 cttttttgtg tgacaaaata aaaacatcta cctattcata tacgctagtg tcatagtcct
      661 gaaaatcatc tgcatcaaga acaatttcac aactcttata cttttctctt acaagtcgtt
      721 cggcttcatc tggattttca gcctctatac ttactaaacg tgataaagtt tctgtaattt
      781 ctactgtatc gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag
      841 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct
      901 tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
      961 tccccgatat gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca
     1021 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc
     1081 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca
     1141 gtccctgttc tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat
     1201 cccttcctga ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat
     1261 ggttgtgctt accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg
     1321 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcac acctcttttt gacatacttc
     1381 gggtatacat atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact
     1441 gttatctggc ttttagtaag ccggatccac gcgattacgc cccgccctgc cactcatcgc
     1501 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat
     1561 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1621 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1681 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1741 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1801 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1861 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt
     1921 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1981 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2041 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2101 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2161 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2221 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat
     2281 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt
     2341 cggcgcaaag tgcgtcgggt gatgctgcca acttagtcga cgacccagct ttcttgtaca
     2401 aagtgggcat taaaagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2461 gtcaaaataa aatcattatt tgctatggtg agcaagggcg aggagctgtt caccggggtg
     2521 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2581 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
     2641 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
     2701 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2761 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2821 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2881 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2941 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     3001 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3061 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     3121 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3181 ggcatggacg agctgtacaa gggcgctgcg gctgctgccg ctgcggcagc ggagttgtta
     3241 atgtgttcgg gtcaggccga gtcaggtggt tcttcttcca ccgagtcttc ttcactcagt
     3301 ggtggactca ggtttggtca gaagatctac ttcgaggatg gatccggatc cagaagcaag
     3361 aaccgggtca ataccgttcg taagtcgtct accacggcga ggtgccaagt ggaaggttgt
     3421 agaatggatc taagcaatgt taaagcttat tactcgagac acaaagtttg ttgcattcac
     3481 tctaaatcat ctaaagtcat tgtctctggt cttcatcaaa ggttttgtca acaatgtagc
     3541 aggtttcccc cttttttctt cttcctttta tttactcatt ttgccatttc cggtgatcgg
     3601 aatagttcta agggaaccat agctttacgc ttttttcagg ttttatgttc cttttgagct
     3661 ttttgagttt tggctcttaa cgtcattgaa gagtcttatc aaaacttaaa ctttacagct
     3721 agttattatg tggtcttgtc tcttgtctta tgcattattt aggttgcttt tctttgaagc
     3781 tttcttttct ttatatcaaa tcaggtttca ccagctttct gagtttgact tggagaaaag
     3841 aagttgtcgc agaagactcg cttgtcataa cgaacgacga agaaaaccac aacccacaac
     3901 ggctcttttc acttctcatt actctcgaat cgctccatct ctttacggtt agatcccaat
     3961 atacacagcc cttttcagct caatgagtct caaaaacaag ttattgaatt tcctgtgtgt
     4021 caggaaaccc caatgctgca atgattaaaa gcgttttggg agatcctact gcgtggtcaa
     4081 ccgcaagatc agtgatgcag cggcctggac cgtggcagat taatccagtt agggaaaccc
     4141 atccacacat gaatgtttta tcacatggaa gctcaagctt tactacatgt ccagagatga
     4201 taaacaacaa tagcacagat tcaagctgtg ctctctctct tctgtcaaac tcatacccaa
     4261 ttcatcagca gcaacttcag acaccaacaa atacatggcg accatcttct ggtttcgact
     4321 cgatgatctc attctccgat aaggttacaa tggctcagcc accgcccatt tcaacccatc
     4381 agccgcccat ctcaacacat cagcagtacc tcagccaaac ttgggaagtc atcgcgggcg
     4441 aaaagagcaa ttcacattat atgtctcctg tgagtcaaat ctcggagcca gcagatttcc
     4501 agataagcaa tggcaccaca atgggtggat ttgagctgta tcttcatcag caggttctga
     4561 agcaatacat ggaacccgag aacacaagag cttatgactc ctctcctcaa catttcaatt
     4621 ggtctctttg agtctaatct ctttcacctt ttaagatctt catcagtttg ttactaaaat
     4681 cttatcaact atctcttgtg tgctacttta aaaccaggca atgatgcccg ataatgcctt
     4741 tccgtttgga tgtttttttc atggcttttt atctttttaa gaactttggt tttattgatg
     4801 atctctacga tagtttacga tttgcatcca acagaaactc aggttacata cttggagatc
     4861 aaattgaaac atcaacatta tcatcaatat attgtcttat caaatcagag ttccacatgc
     4921 ttttatcatc ctactatcta tttaaaagca tgagaattag caagtcctta agattctaag
     4981 tgtgtacctc aactgcaaac acagtcttcc ttctgtatct tgcttcctct tcagccacat
     5041 caggaaattt tcaacctgaa gcttctgcag acatggattg tgtttgtgtg cttccatact
     5101 agtgtggctt atccatccag ctgcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     5161 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     5221 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     5281 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     5341 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     5401 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     5461 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     5521 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     5581 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     5641 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     5701 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     5761 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     5821 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     5881 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     5941 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     6001 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     6061 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     6121 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6181 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6241 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6301 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6361 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6421 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6481 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6541 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6601 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6661 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6721 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6781 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
