LOCUS       V012737                13457 bp    DNA     circular SYN 11-SEP-2021
DEFINITION  Exported.
ACCESSION   V012737
VERSION     V012737
KEYWORDS    TetO-FUW-OSKM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 13457)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..656
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             671..851
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    898..1023
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1516..1749
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1934..1978
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2127..2168
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2276..2393
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    2446..2716
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        2749..2786
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2889..3944
                     /label="mPou5f1"
                     /note="Mus musculus Oct-4 gene. Encodes a transcription
                     factor containing a POU homeodomain that plays a key role
                     in embryonic development and stem cell pluripotency."
     CDS             3960..4016
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4023..4979
                     /label="mSox2"
                     /note="Mus musculus transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of
                     transcription factors, which is involved in the regulation
                     of embryonic development and in the determination of cell
                     fate"
     CDS             4995..5048
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="2A peptide from Thosea asigna virus capsid protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5055..6503
                     /label="mKlf4"
                     /note="Mouse Kruppel-like factor (Klf4) gene. Belongs to
                     the relatively large family of SP1-like transcription
                     factors and is involved in the regulation of proliferation,
                     differentiation, apoptosis and somatic cell reprogramming."
     CDS             6519..6578
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus
                     polyprotein"
                     /label="2A peptide from equine rhinitis A virus polypro"
                     /note="E2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6588..7949
                     /label="mMyc"
                     /note="Mus musculus myc proto-oncogene. Belongs to
                     myelocytomatosis (Myc) family of transcription factors.
                     Plays a role in cell cycle procession, apotosis and
                     cellular transformation"
     misc_feature    7985..8573
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(8576..8592)
                     /label="KS primer"
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             9098..9278
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9310..9534
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9580..10000
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10022..10351
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        10399..10446
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             10465..10836
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    10969..11102
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11139..11155)
                     /label="M13 rev"
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    11163..11179
                     /label="lac repressor encoded by lacI binding site"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11187..11217)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11232..11253)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11541..12129)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12303..13160)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13161..13265)
                     /label="AmpR promoter"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      661 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      721 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      781 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      841 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      901 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      961 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1021 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1081 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1141 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1201 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1261 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1321 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1381 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1441 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1501 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1561 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1621 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1681 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1741 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1801 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1861 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1921 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1981 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2041 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2101 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2161 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2221 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     2281 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2341 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2401 attacaggga cagcagagat ccagtttggt tatcgagttt accactccct atcagtgata
     2461 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2521 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2581 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2641 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2701 ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagt aggcgtgtac
     2761 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     2821 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg
     2881 aattcgccat ggctggacac ctggcttcag acttcgcctt ctcaccccca ccaggtgggg
     2941 gtgatgggtc agcagggctg gagccgggct gggtggatcc tcgaacctgg ctaagcttcc
     3001 aagggcctcc aggtgggcct ggaatcggac caggctcaga ggtattgggg atctccccat
     3061 gtccgcccgc atacgagttc tgcggaggga tggcatactg tggacctcag gttggactgg
     3121 gcctagtccc ccaagttggc gtggagactt tgcagcctga gggccaggca ggagcacgag
     3181 tggaaagcaa ctcagaggga acctcctctg agccctgtgc cgaccgcccc aatgccgtga
     3241 agttggagaa ggtggaacca actcccgagg agtcccagga catgaaagcc ctgcagaagg
     3301 agctagaaca gtttgccaag ctgctgaagc agaagaggat caccttgggg tacacccagg
     3361 ccgacgtggg gctcaccctg ggcgttctct ttggaaaggt gttcagccag accaccatct
     3421 gtcgcttcga ggccttgcag ctcagcctta agaacatgtg taagctgcgg cccctgctgg
     3481 agaagtgggt ggaggaagcc gacaacaatg agaaccttca ggagatatgc aaatcggaga
     3541 ccctggtgca ggcccggaag agaaagcgaa ctagcattga gaaccgtgtg aggtggagtc
     3601 tggagaccat gtttctgaag tgcccgaagc cctccctaca gcagatcact cacatcgcca
     3661 atcagcttgg gctagagaag gatgtggttc gagtatggtt ctgtaaccgg cgccagaagg
     3721 gcaaaagatc aagtattgag tattcccaac gagaagagta tgaggctaca gggacacctt
     3781 tcccaggggg ggctgtatcc tttcctctgc ccccaggtcc ccactttggc accccaggct
     3841 atggaagccc ccacttcacc acactctact cagtcccttt tcctgagggc gaggcctttc
     3901 cctctgttcc cgtcactgct ctgggctctc ccatgcattc aaacgctagc ggcagcggcg
     3961 ccacaaactt ctctctgcta aagcaagcag gtgatgttga agaaaacccc gggcctgcat
     4021 gcatgtataa catgatggag acggagctga agccgccggg cccgcagcaa gcttcggggg
     4081 gcggcggcgg aggaggcaac gccacggcgg cggcgaccgg cggcaaccag aagaacagcc
     4141 cggaccgcgt caagaggccc atgaacgcct tcatggtatg gtcccggggg cagcggcgta
     4201 agatggccca ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgcgg
     4261 agtggaaact tttgtccgag accgagaagc ggccgttcat cgacgaggcc aagcggctgc
     4321 gcgctctgca catgaaggag cacccggatt ataaataccg gccgcggcgg aaaaccaaga
     4381 cgctcatgaa gaaggataag tacacgcttc ccggaggctt gctggccccc ggcgggaaca
     4441 gcatggcgag cggggttggg gtgggcgccg gcctgggtgc gggcgtgaac cagcgcatgg
     4501 acagctacgc gcacatgaac ggctggagca acggcagcta cagcatgatg caggagcagc
     4561 tgggctaccc gcagcacccg ggcctcaacg ctcacggcgc ggcacagatg caaccgatgc
     4621 accgctacga cgtcagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga
     4681 acggctcgcc cacctacagc atgtcctact cgcagcaggg cacccccggt atggcgctgg
     4741 gctccatggg ctctgtggtc aagtccgagg ccagctccag cccccccgtg gttacctctt
     4801 cctcccactc cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtacc
     4861 tccccggcgc cgaggtgccg gagcccgctg cgcccagtag actgcacatg gcccagcact
     4921 accagagcgg cccggtgccc ggcacggcca ttaacggcac actgcccctg tcgcacatgg
     4981 catgcggctc cggcgagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc
     5041 ccggcccact cgagatgagg cagccacctg gcgagtctga catggctgtc agcgacgctc
     5101 tgctcccgtc cttctccacg ttcgcgtccg gcccggcggg aagggagaag acactgcgtc
     5161 cagcaggtgc cccgactaac cgttggcgtg aggaactctc tcacatgaag cgacttcccc
     5221 cacttcccgg ccgcccctac gacctggcgg cgacggtggc cacagacctg gagagtggcg
     5281 gagctggtgc agcttgcagc agtaacaacc cggccctcct agcccggagg gagaccgagg
     5341 agttcaacga cctcctggac ctagacttta tcctttccaa ctcgctaacc caccaggaat
     5401 cggtggccgc caccgtgacc acctcggcgt cagcttcatc ctcgtcttcc ccagcgagca
     5461 gcggccctgc cagcgcgccc tccacctgca gcttcagcta tccgatccgg gccgggggtg
     5521 acccgggcgt ggctgccagc aacacaggtg gagggctcct ctacagccga gaatctgcgc
     5581 cacctcccac ggcccccttc aacctggcgg acatcaatga cgtgagcccc tcgggcggct
     5641 tcgtggctga gctcctgcgg ccggagttgg acccagtata cattccgcca cagcagcctc
     5701 agccgccagg tggcgggctg atgggcaagt ttgtgctgaa ggcgtctctg accacccctg
     5761 gcagcgagta cagcagccct tcggtcatca gtgttagcaa aggaagccca gacggcagcc
     5821 accccgtggt agtggcgccc tacagcggtg gcccgccgcg catgtgcccc aagattaagc
     5881 aagaggcggt cccgtcctgc acggtcagcc ggtccctaga ggcccatttg agcgctggac
     5941 cccagctcag caacggccac cggcccaaca cacacgactt ccccctgggg cggcagctcc
     6001 ccaccaggac tacccctaca ctgagtcccg aggaactgct gaacagcagg gactgtcacc
     6061 ctggcctgcc tcttccccca ggattccatc cccatccggg gcccaactac cctcctttcc
     6121 tgccagacca gatgcagtca caagtcccct ctctccatta tcaagagctc atgccaccgg
     6181 gttcctgcct gccagaggag cccaagccaa agaggggaag aaggtcgtgg ccccggaaaa
     6241 gaacagccac ccacacttgt gactatgcag gctgtggcaa aacctatacc aagagttctc
     6301 atctcaaggc acacctgcga actcacacag gcgagaaacc ttaccactgt gactgggacg
     6361 gctgtgggtg gaaattcgcc cgctccgatg aactgaccag gcactaccgc aaacacacag
     6421 ggcaccggcc ctttcagtgc cagaagtgcg acagggcctt ttccaggtcg gaccaccttg
     6481 ccttacacat gaagaggcac tttctcgagg gctcgggcca gtgtactaat tatgctctct
     6541 tgaaattggc tggagatgtt gagagcaacc caggtcccgg cgcgccactg gatttccttt
     6601 gggcgttgga aaccccgcag acagccacga cgatgcccct caacgtgaac ttcaccaaca
     6661 ggaactatga cctcgactac gactccgtac agccctattt catctgcgac gaggaagaga
     6721 atttctatca ccagcaacag cagagcgagc tgcagccgcc cgcgcccagt gaggatatct
     6781 ggaagaaatt cgagctgctt cccaccccgc ccctgtcccc gagccgccgc tccgggctct
     6841 gctctccatc ctatgttgcg gtcgctacgt ccttctcccc aagggaagac gatgacggcg
     6901 gcggtggcaa cttctccacc gccgatcagc tggagatgat gaccgagtta cttggaggag
     6961 acatggtgaa ccagagcttc atctgcgatc ctgacgacga gaccttcatc aagaacatca
     7021 tcatccagga ctgtatgtgg agcggtttct cagccgctgc caagctggtc tcggagaagc
     7081 tggcctccta ccaggctgcg cgcaaagaca gcaccagcct gagccccgcc cgcgggcaca
     7141 gcgtctgctc cacctccagc ctgtacctgc aggacctcac cgccgccgcg tccgagtgca
     7201 ttgacccctc agtggtcttt ccctacccgc tcaacgacag cagctcgccc aaatcctgta
     7261 cctcgtccga ttccacggcc ttctctcctt cctcggactc gctgctgtcc tccgagtcct
     7321 ccccacgggc cagccctgag cccctagtgc tgcatgagga gacaccgccc accaccagca
     7381 gcgactctga agaagagcaa gaagatgagg aagaaattga tgtggtgtct gtggagaaga
     7441 ggcaaacccc tgccaagagg tcggagtcgg gctcatctcc atcccgaggc cacagcaaac
     7501 ctccgcacag cccactggtc ctcaagaggt gccacgtctc cactcaccag cacaactacg
     7561 ccgcaccccc ctccacaagg aaggactatc cagctgccaa gagggccaag ttggacagtg
     7621 gcagggtcct gaagcagatc agcaacaacc gcaagtgctc cagccccagg tcctcagaca
     7681 cggaggaaaa cgacaagagg cggacacaca acgtcttgga acgtcagagg aggaacgagc
     7741 tgaagcgcag cttttttgcc ctgcgtgacc agatccctga attggaaaac aacgaaaagg
     7801 cccccaaggt agtgatcctc aaaaaagcca ccgcctacat cctgtccatt caagcagacg
     7861 agcacaagct cacctctgaa aaggacttat tgaggaaacg acgagaacag ttgaaacaca
     7921 aactcgaaca gcttcgaaac tctggtgcat aaggcgcgcc gaattcgata tcaagcttat
     7981 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     8041 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     8101 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     8161 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     8221 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     8281 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     8341 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     8401 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     8461 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8521 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     8581 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     8641 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     8701 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8761 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     8821 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     8881 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     8941 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     9001 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     9061 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     9121 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9181 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9241 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     9301 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     9361 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     9421 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     9481 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     9541 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     9601 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9661 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9721 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9781 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9841 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9901 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9961 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
    10021 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10081 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    10141 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    10201 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    10261 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
    10321 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
    10381 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
    10441 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
    10501 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
    10561 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
    10621 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
    10681 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
    10741 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
    10801 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
    10861 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    10921 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    10981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    11101 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    11161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    11221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    11281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    11341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    12001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    12061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    12121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    12181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    12301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    12361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    12421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    12481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    13021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    13081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    13141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    13201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13261 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
    13321 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
    13381 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
    13441 ggcaaggctt gaccgac
//
