LOCUS       V013444                 9185 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013444
VERSION     V013444
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1023
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     LTR             1098..1331
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1403..1537
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1564..1699
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(1720..1738)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1748..1764)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1906..2361
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2387..2491
                     /label="AmpR promoter"
     CDS             2492..3349
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3523..4111
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4399..4420
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4435..4465
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4473..4489
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4497..4513
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4534..4552
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4580..4806
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4807..4987
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5034..5159
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    5652..5885
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6070..6114
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6263..6304
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        6403..6497
                     /label="H1-2O2 promoter"
                     /note="doxycycline-inducible variant of the human H1 RNA
                     promoter (Henriksen et al., 2007)"
     misc_feature    6597..6714
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        6763..7273
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     intron          7282..7854
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     promoter        7909..7927
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7938..8558
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     misc_feature    8593..9166
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
       61 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
      121 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
      181 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
      241 ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
      301 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
      361 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
      421 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
      481 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
      541 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
      601 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
      661 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
      721 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
      781 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
      841 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
      901 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
      961 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     1021 gaatagggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     1081 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     1141 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     1201 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     1261 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1321 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     1381 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     1441 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     1501 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     1561 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     1621 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     1681 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     1741 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1801 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1861 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     1921 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1981 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2041 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2101 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2161 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2221 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2281 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2341 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2461 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2521 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2581 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2641 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2701 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2761 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2821 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2881 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2941 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3001 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3061 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3121 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3181 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3241 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3301 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4201 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4261 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4321 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4381 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4441 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4501 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     4561 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     4621 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     4681 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     4741 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     4801 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4861 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4921 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4981 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     5041 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     5101 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     5161 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     5221 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     5281 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     5341 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     5401 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     5461 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     5521 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     5581 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     5641 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     5701 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     5761 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     5821 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     5881 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     5941 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     6001 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     6061 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     6121 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     6181 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     6241 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     6301 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     6361 tctcgacggt atcgatcacg agactagcct cgagcggccg caatatttgc atgtcgctat
     6421 gtgttctggg aaatcaccat aaacgtgaaa tccctatcag tgatagagac ttataagttc
     6481 cctatcagtg atagagacac cggcctaagg ttaagtcgcc ctcgctcgag cgagggcgac
     6541 ttaaccttag gtttttaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
     6601 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     6661 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     6721 tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct
     6781 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     6841 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     6901 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     6961 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     7021 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     7081 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     7141 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     7201 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     7261 cctctctccc cagggggatc tgtgagtttg gggacccttg attgttcttt ctttttcgct
     7321 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
     7381 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
     7441 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
     7501 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
     7561 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
     7621 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
     7681 tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt catcatcctg
     7741 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
     7801 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     7861 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgta atacgactca
     7921 ctatagggcg aattgatatg tctagattag ataaaagtaa agtgattaac agcgcattag
     7981 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
     8041 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
     8101 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
     8161 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
     8221 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
     8281 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
     8341 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
     8401 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
     8461 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
     8521 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagcggc tcccgggagt
     8581 tctagggatc tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     8641 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     8701 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     8761 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     8821 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     8881 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     8941 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     9001 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     9061 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     9121 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataaggat ccaccggagc
     9181 ttacc
//
