LOCUS       V013552                11003 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013552
VERSION     V013552
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     intron          516..1088
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     promoter        1143..1161
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1172..1792
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     misc_feature    1827..2400
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2420..3442
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     LTR             3517..3750
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3822..3956
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3983..4118
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(4139..4157)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4325..4780
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4806..4910
                     /label="AmpR promoter"
     CDS             4911..5768
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5942..6530
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6818..6839
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6854..6884
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        6953..6971
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6999..7225
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7226..7406
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7453..7578
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8071..8304
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8489..8533
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8682..8723
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        8822..8916
                     /label="H1-2O2 promoter"
                     /note="doxycycline-inducible variant of the human H1 RNA
                     promoter (Henriksen et al., 2007)"
     promoter        9999..10507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     misc_feature    10834..10951
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt
      541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
      841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
      901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc
      961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt
     1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat
     1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
     1801 cggctcccgg gagttctagg gatctgcccc tctccctccc ccccccctaa cgttactggc
     1861 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     1921 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     1981 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     2041 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     2101 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     2161 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     2221 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     2281 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     2341 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     2401 ggatccaccg gagcttacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     2461 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     2521 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     2581 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     2641 tccggaagtg cttgacattg gggagttcag cgagagcctg acctattgca tctcccgccg
     2701 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     2761 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     2821 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     2881 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     2941 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     3001 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     3061 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     3121 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     3181 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     3241 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     3301 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     3361 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     3421 cactcgtccg agggcaaagg aatagggtac ctttaagacc aatgacttac aaggcagctg
     3481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3721 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     3781 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3961 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4021 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4081 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4141 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4201 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4261 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4321 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4381 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4621 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4681 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4741 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4801 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4861 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4921 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4981 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5041 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5101 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5161 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5221 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5281 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5341 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5401 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5461 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5521 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5581 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5641 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5701 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5761 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5821 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5881 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5941 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6001 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6061 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6121 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6181 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6241 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6301 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6361 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6421 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6481 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6541 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6601 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6661 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6721 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6781 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6841 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6901 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6961 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     7021 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7081 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7141 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7201 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7381 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7441 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7501 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7561 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7621 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7681 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7741 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7801 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7861 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7921 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7981 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8041 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8101 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8161 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8221 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8281 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8341 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8401 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8461 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8521 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8581 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8641 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8701 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     8761 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gagcggccgc
     8821 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat ccctatcagt
     8881 gatagagact tataagttcc ctatcagtga tagagacacc ggtgtgttgt aaatgagcac
     8941 acaaaataca catgctaaaa tattatattc tatgaccttt ataaaatcaa ccaaaatctt
     9001 ctttttaata actttagtat caataattag aatttttatg ttcctttttg caaactttta
     9061 ataaaaatga gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct
     9121 ttaataatag ctactccacc acttgttcct aagcggtcag ctcctgcttc aatcattttt
     9181 tgagcatctt caaatgttct aactccacca gctgctttaa ctaaagcatt gtctttaaca
     9241 actgacttca ttagtttaac atcttcaaat gttgcacctg attttgaaaa tcctgttgat
     9301 gttttaacaa attctaatcc agcttcaaca gctatttcac aagctttcat gatttcttct
     9361 tttgttaata aacaattttc cataatacat ttaacaacat gtgatccagc tgcttttttt
     9421 acagctttca tgtcttctaa aactaattca taatttttgt cttttaatgc accaatattt
     9481 aataccatat caatttctgt tgcaccatct ttaattgctt cagaaacttc gaatgctttt
     9541 gtagctgttg tgcatgcacc tagaggaaaa cctacaacat ttgttattcc tacatttgtg
     9601 ccttttaata attctttaca atagcttgtt caatatgaat taacacaaac tgttgcaaaa
     9661 tcaaattcaa ttgcttcatc acataattgt ttaatttcag ctttcgtagc atcttgtttt
     9721 aataatgtgt gatctatata tttgtttagt ttcatttttt ctcctatata ttcattttta
     9781 attttaattc tttaataatt tcgtctactt taactttagc gttttgaaca gattcaccaa
     9841 cacctataaa ataaattttt agtttaggtt cagttccact tgggcgaaca gcaaatcatg
     9901 acttatcttc taaataaaat tttagtaagt cttgtcctgg catattatac attccatcga
     9961 tgtagtcttc aacattaaca actttaagtc cagcaatttg agttaagggt gttgctctca
    10021 atgatttcat taatggttca atttttaatt tcttttcttc tggtttaaaa ttcaagttta
    10081 aagtgaaagt gtaatatgca cccatttctt taaataaatc ttctaaatag tctactaatg
    10141 ttttattttg ttttttataa aatcaagcag cctctgctat taatatagaa gcttgtattc
    10201 catctttatc tctagctgag tcatcaatta catatccata actttcttca taagcaaaaa
    10261 caaaatttaa tccgttatct tcttctttag caatttctct acccattcat ttaaatccag
    10321 ttaaagtttt tacaatatta actccatatt tttcatgagc gattctatca cccaaatcac
    10381 ttgttacaaa acttgaatat agagccggat tttttggaat gctatttaag cgttttagat
    10441 ttgataattt tcaatcaatt aaaattggtc ctgtttgatt tccatctaat cttacaaaat
    10501 gaccatcatg ttttattgcc attccaaatc tgtcagcatc tgggtcattc ataataataa
    10561 tatctgcatc atgtttaata ccatattcaa gcggtatttt tcatgcagga tcaaattctg
    10621 gatttggatt tacaacattt ttaaatgttt catcttcaaa tgcatgctct tcaacctcaa
    10681 taacgttata tcctgattca cgtaatattt ttggggtaaa tttagttcct gttccattaa
    10741 ctgcgctaaa aataattttt aaatcttttt tagcttcttg ctcttttttg tagaattctc
    10801 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
    10861 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    10921 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    10981 actttggccg cggctcgagg ggg
//
