LOCUS       40924_48962       12953 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary expression vector TP-D3cpv, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     FRET-based genetically encoded sensors allow high-resolution live 
            cell imaging of Ca(2) dynamics
  JOURNAL   Plant J. 69 (1), 181-192 (2012)
  PUBMED    21910770
REFERENCE   2  (bases 1 to 12953)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2011) Department of Developmental Biology, Centre 
            for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 12953)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12953)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "1"; pages: "181-192"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2011) Department of Developmental Biology, Centre for 
            Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 
            69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    303..324
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        339..369
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    377..393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     401..417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      445..1049
                     /label=AtUBQ10
                     /note="AtUBQ10"
                     /regulatory_class="promoter"
     misc_feature    1101..1167
                     /label=TP anchor, N-terminal peptide of AtCBL2
                     /note="TP anchor, N-terminal peptide of AtCBL2"
     CDS             1179..1862
                     /label=CFP
                     /note="cyan variant of GFP"
     CDS             2619..3137
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     terminator      3256..3897
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3910..3926)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4508..4760)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4959..5750)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5780..6160)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    complement(6532..6556)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7084..7872
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      8119..8707
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8893..9033)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9377..9571)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9640..10704)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11141..11767)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      241 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      301 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      361 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      421 gattacgaat tcgagctcgg tacccgacga gtcagtaata aacggcgtca aagtggttgc
      481 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
      541 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
      601 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
      661 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
      721 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
      781 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
      841 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
      901 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
      961 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt
     1021 tagtttctag tttgtgcgat cgaatttgta gattaatctg agtttttctg attaacagct
     1081 cgagtgcggg atccactagt atgtcgcagt gcgttgacgg tatcaagcat ttatgcacct
     1141 ctgttttagg gtgttttgat cttgataagc ttgccaccat ggtgagcaag ggcgaggagc
     1201 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaggt
     1261 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1321 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     1381 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1441 ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac ggcaactaca
     1501 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1561 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
     1621 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc cacttcaaga
     1681 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1741 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1801 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1861 cccgcatgca tgaccaactg acagaagagc agattgcaga gttcaaagaa gccttctcat
     1921 tattggacaa ggatggggac ggcaccatca ccacaaagga acttggcacc gctctgaggt
     1981 cgcttggaca aaacccaacg gaagcagaat tgcaggatat gatcaatgaa gtcgatgctg
     2041 atggcaatgg aacgatttac tttcctgaat ttcttactat gatggctaga aaaatgaagg
     2101 acacagacag cgaagaggaa atccgagaag cattccgtgt ttttgacaag gatgggaacg
     2161 gctacatcag cgctgctgaa ttacgtcacg tcatgacaaa cctcggggag aagttaacag
     2221 atgaagaagt tgatgaaatg ataagggaag cagatatcga tggtgatggc caagtaaact
     2281 atgaagagtt tgtacaaatg atgacagcaa agggggggaa gaggcgctgg cagaaaacag
     2341 gccatgctgt ccgagcattc ggaagactga agaagatctc cagctccggg gcactggagc
     2401 tcatggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2461 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     2521 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2581 tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag ggcgaggagc
     2641 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     2701 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     2761 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     2821 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2881 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     2941 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3001 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3061 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     3121 tccgccacaa catcgagtaa gaattctgca gatatccatc acactggcgg ccgctcgagc
     3181 atgcatctag agggcccggg acgtccgcgg agatctacgc gtgtcgactc gagatatcca
     3241 actagttggc tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca
     3301 atgcatcagt ttcattgcgc acacaccaga atcctactga gttcgagtat tatggcattg
     3361 ggaaacatgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
     3421 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
     3481 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
     3541 ttgaaaatat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
     3601 ctctaatgac cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat
     3661 tcactaggca acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag
     3721 tatgtcctct tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct
     3781 tgtcagattc taatcattgc tttataatta tagttatact catggatttg tagttgagta
     3841 tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaatcg
     3901 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3961 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4021 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgcc
     4081 aatcgaggcc gaccaaccgc aagcgttgtc agtgttgcaa agcgctctgt gtgggcctac
     4141 tttaattgct tccagtgtta aattggcgaa aggcaataat atcgcaaaat attgtgttgt
     4201 aaaatgtaat tatgttttaa tttcatggaa atgtttgagc ataattttta ttaatgtact
     4261 aaattactgt tttgttaaat gcaattttgc tttctcggga ttttaatatc aaaatctatt
     4321 tagaaataca caatattttg ttgcaggctt gctggagaat cgatctgcta tcataaaaat
     4381 tacaaaaaaa ttttatttgc ctcaattatt ttaggattgg tattaaggac gcttaaatta
     4441 tttgtcgggt cactacgcat cattgtgatt gagaagatca gcgatacgaa atattcgtag
     4501 tactatcgat aatttatttg aaaattcata agaaaagcaa acgttacatg aattgatgaa
     4561 acaatacaaa gacagataaa gccacgcaca tttaggatat tggccgagat tactgaatat
     4621 tgagtaagat cacggaattt ctgacaggag catgtcttca attcagccca aatggcagtt
     4681 gaaatactca aaccgcccca tatgcaggag cggatcattc attgtttgtt tggttgcctt
     4741 tgccaacatg ggagtccaag gtttcaggga agctggaatt ccggggtggg cgaagaactc
     4801 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     4861 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     4921 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     4981 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     5041 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     5101 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     5161 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
     5221 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     5281 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     5341 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     5401 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     5461 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     5521 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc
     5581 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     5641 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     5701 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     5761 ccggggaatt cgctagagtc gatttggtgt atcgagattg gttatgaaat tcagatgcta
     5821 gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt atccatttct
     5881 gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc tgtaaagcgt
     5941 tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt caaaagtcgt
     6001 taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga aattcaggcc
     6061 cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa taaaaactga
     6121 ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcaatat aaacaaagac
     6181 ggccacaaga aaaaaccaaa acaccgatat tcattaatct tatctagttt ctcaaaaaaa
     6241 ttcatatctt ccacacgtga aaatgccaat ttctcagacc tacctcggct ctgcgaaggc
     6301 ccccgctggt atcaaaagtt tttatttcat ccgacatggc gcgaccgacc tcaacgagaa
     6361 ggaaattgtc gtgaacggtg agaagctctg gggcgtgcaa ggttccggaa cgaacatcgg
     6421 tctcaatgca aaaggggaac gccaggctct gttggcccct cgaaattcgg cgttaattca
     6481 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     6541 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     6601 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     6661 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     6721 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     6781 gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctcgttc ggttcgtaag
     6841 ctgtaatgca agtagcgtaa ctgccgtcac gcaactggtc cagaaccttg accgaacgca
     6901 gcggtggtaa cggcgcagtg gcggttttca tggcttcttg ttatgacatg tttttttggg
     6961 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     7021 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     7081 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     7141 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     7201 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     7261 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     7321 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     7381 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     7441 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     7501 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     7561 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     7621 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     7681 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     7741 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     7801 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     7861 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgccggcgt
     7921 taactcaagc gattagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     7981 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     8041 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8101 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8161 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8221 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     8281 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8341 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8401 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8461 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8521 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8581 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8641 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8701 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8761 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8821 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8881 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8941 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     9001 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     9061 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     9121 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     9181 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     9241 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     9301 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     9361 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     9421 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     9481 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     9541 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     9601 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     9661 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     9721 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     9781 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     9841 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     9901 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     9961 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
    10021 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
    10081 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
    10141 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
    10201 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
    10261 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
    10321 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
    10381 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
    10441 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
    10501 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
    10561 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
    10621 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
    10681 taccgggccg gatggtttgc gaccgtcacg ccgattcctc gggcttgggg gttccagtgc
    10741 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
    10801 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
    10861 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
    10921 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
    10981 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
    11041 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
    11101 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
    11161 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
    11221 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
    11281 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
    11341 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
    11401 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
    11461 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
    11521 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
    11581 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
    11641 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
    11701 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
    11761 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
    11821 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
    11881 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
    11941 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
    12001 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
    12061 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
    12121 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
    12181 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
    12241 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
    12301 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
    12361 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
    12421 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
    12481 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
    12541 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
    12601 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
    12661 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
    12721 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
    12781 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
    12841 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
    12901 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt act
//
