LOCUS       40924_48942        7827 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector TKVBL-w+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Kondo S, Booker M, Perrimon N.
  TITLE     Cross-species RNAi rescue platform in Drosophila melanogaster
  JOURNAL   Genetics 183 (3), 1165-1173 (2009)
  PUBMED    19720858
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Kondo S, Booker M, Perrimon N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2009) Department of Genetics, Harvard Medical 
            School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 7827)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2009"; volume: "183"; issue: "3"; pages: "1165-1173"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2009) Department of Genetics, Harvard Medical School, 77 
            Avenue Louis Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1107..1140
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(1898..1969,2364..2637,2712..3366,3428..3743,3964..4095,
                     4166..4780)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     CDS             complement(5738..6550)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      6674..7309
                     /label=oriV
                     /note="incP origin of replication"
     protein_bind    7344..7413
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     mobile_element  complement(7639..7804)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ggtaccctgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
       61 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
      121 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      181 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      241 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      301 cagcgccctg caccattatg ttccggatct atgtcgggtg cggagaaaga ggtaatgaaa
      361 tggcagatcc ctggcttgtt gtccacaacc gttaaacctt aaaagcttta aaagccttat
      421 atattctttt ttttcttata aaacttaaaa ccttagaggc tatttaagtt gctgatttat
      481 attaatttta ttgttcaaac atgagagctt agtacgtgaa acatgagagc ttagtacgtt
      541 agccatgaga gcttagtacg ttagccatga gggtttagtt cgttaaacat gagagcttag
      601 tacgttaaac atgagagctt agtacgtgaa acatgagagc ttagtacgta ctatcaacag
      661 gttgaactgc tgatcttcgg atctatgtcg ggtgcggaga aagaggtaat gaaatggcag
      721 atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc
      781 ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat
      841 tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat
      901 gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt
      961 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa
     1021 ctgctgatct tcggatctat gtcgggtgcg gagaaagagg taatgaaatg gcatccggat
     1081 ctgcatcgca ggatgctgct ggctacataa cttcgtataa tgtatgctat acgaagttat
     1141 ctgtcatgcc atccgtatgt gggcggacaa taaagtctta aactgaacaa aatagatcta
     1201 aactatgaca ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt
     1261 atgctgtgaa aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga
     1321 agtgcaaatt gcccgtcgta ttaaagaggg gcgtggggtc gacgcggccg ctaactataa
     1381 cggtcctaag gtagcgagtt taaacgatcc ttagtatgta tgtaagttaa taaaaccctt
     1441 ttttggagaa tgtagattta aaaaaacata tttttttttt attttttact gcactggaca
     1501 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgaagtacca
     1561 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     1621 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     1681 gccaccgttt gtagcgttac ctagcgtcaa tgtccgcctt cagttgcact ttgtcagcgg
     1741 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
     1801 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
     1861 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     1921 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
     1981 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
     2041 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caatcgcaca aacaaatagt
     2101 gacacgaaac agattattct ggtagctgtg ctcgctatat aagacaattt ttaagatcat
     2161 atcatgatca agacatctaa aggcattcat tttcgactac attctttttt acaaaaaata
     2221 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
     2281 tacttcgtag gatacttcgt tttgttcggg gttagatgag cataacgctt gtagttgata
     2341 tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc tgcattaacc
     2401 agggcttcgg gcaggccaaa aactacggca cgctcctgcc acccagtccg ccggaggact
     2461 ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca
     2521 tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac cggacacgcg
     2581 gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg
     2641 agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat
     2701 ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc
     2761 cggtgccgga aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc agggcatcca
     2821 agtatcgcca tccgggatgc gactgctcaa tggccaacct gtggacgcca aggagatgca
     2881 ggccaggtgc gcctatgtcc agcaggatga cctctttatc ggctccctaa cggccaggga
     2941 acacctgatt ttccaggcca tggtgcggat gccacgacat ctgacctatc ggcagcgagt
     3001 ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc aaatgtcagc acacgatcat
     3061 cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc
     3121 ctccgaggca ctaaccgatc cgccgcttct gatctgcgat gagcccacct ccggactgga
     3181 ctcatttacc gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac
     3241 cgtcatcctg accattcatc agccgtcttc cgagctgttt gagctctttg acaagatcct
     3301 tctgatggcc gagggcaggg tagctttctt gggcactccc agcgaagccg tcgacttctt
     3361 ttcctagtga gttcgatgtg tttattaagg gtatctagca ttacattaca tctcaactcc
     3421 tatccagcgt gggtgcccag tgtcctacca actacaatcc ggcggacttt tacgtacagg
     3481 tgttggccgt tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc aagatatgcg
     3541 acaattttgc tattagcaaa gtagcccggg atatggagca gttgttggcc accaaaaatt
     3601 tggagaagcc actggagcag ccggagaatg ggtacaccta caaggccacc tggttcatgc
     3661 agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa
     3721 aagtgcgact tattcagaca acggtgagtg gttccagtgg aaacaaatga tataacgctt
     3781 acaattcttg gaaacaaatt cgctagattt tagttagaat tgcctgattc cacacccttc
     3841 ttagtttttt tcaatgagat gtatagttta tagttttgca gaaaataaat aaatttcatt
     3901 taactcgcga acatgttgaa gatatgaata ttaatgagat gcgagtaaca ttttaatttg
     3961 cagatggttg ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggc
     4021 gtgatgaata tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc
     4081 tttgccacga taaatgtaag tcttgtttag aatacatttg catattaata atttactaac
     4141 tttctaatga atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga
     4201 ggcccgaagt cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt
     4261 accgcttttt ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact
     4321 gcgggccgga gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt
     4381 gtcaacgtcc ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc
     4441 tgtgggtccg ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg
     4501 ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga
     4561 gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc
     4621 gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc
     4681 cgatctgccg ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc
     4741 atatctggct ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa
     4801 ctgcttgttt ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa
     4861 aagctaatgt aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta
     4921 tttccccttc gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta
     4981 cacacctgca taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat
     5041 tttttatata catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca
     5101 cgtttcgtag ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg
     5161 ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc
     5221 cgagtggttc atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt
     5281 tgctccaata tatttgtata tatttccctc acagctatat ttattctaat ttaatattat
     5341 gactttttaa ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg
     5401 aaagtgacat ccagtgtttg ttccttgtgt agaatcggat ccggccgccg ctgaggtctg
     5461 cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc atccagccag
     5521 aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg
     5581 aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc
     5641 aactcagcaa gagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc
     5701 tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat
     5761 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     5821 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     5881 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     5941 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct
     6001 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     6061 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     6121 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     6181 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     6241 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     6301 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     6361 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     6421 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     6481 ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa
     6541 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     6601 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtgctcga
     6661 aattcccctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
     6721 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccggc
     6781 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
     6841 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
     6901 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
     6961 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
     7021 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
     7081 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
     7141 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
     7201 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
     7261 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct
     7321 cggtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg
     7381 ggctccccgg gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg
     7441 tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg
     7501 ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg
     7561 cggggcagcg tcagcgggtt ctcgacggtc acggcgggca tgtcgatcac gatgagaatg
     7621 gccagactag tgtcgaccaa ccagataagt gaaatctagt tccaaactat tttgtcattt
     7681 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
     7741 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
     7801 cacaccgtaa gatgcttttc tgtgact
//
