LOCUS       TAGZyme_pQE-1.        3466 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for bacterial expression of 6xHis-tagged proteins that can be
            processed by TAGZyme.
ACCESSION   .
VERSION     .
KEYWORDS    TAGZyme pQE-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     A blunt coding sequence can be inserted at the PvuII site, allowing 
            TAGZyme to remove the 6xHis tag and subsequent glutamine.
FEATURES             Location/Qualifiers
     source          1..3466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             121..138
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    139..197
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    197..207
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      213..307
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             351..1007
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1075..1161
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1316..1456
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1642..2230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2404..3261)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3262..3366)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaaa
      121 catcaccatc accatcacca gctgcatgcg ggagctcatg cggccgcggg tacccacgtg
      181 tcgacctgca gaagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac
      241 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
      301 tgagaatcca agctagcttg gcgagatttt caggagctaa ggaagctaaa atggagaaaa
      361 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      421 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctcgat attacggcct
      481 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      541 cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga
      601 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
      661 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
      721 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
      781 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
      841 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
      901 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
      961 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     1021 gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag gcatcaaata
     1081 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1141 gctctcctga gtaggacaaa tccgccctct agagctgcct cgcgcgtttc ggtgatgacg
     1201 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     1261 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     1321 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     1381 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     1441 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1501 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1561 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1621 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1681 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1741 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1801 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1861 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1921 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1981 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2041 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     2101 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2161 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2221 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2281 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2341 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2401 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2461 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2521 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2581 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2641 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2701 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2761 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2821 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2881 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2941 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3001 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3061 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3121 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3181 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3241 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3301 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3361 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3421 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac
//
